Journal of Pathology and Translational Medicine

Original Articles

Immunohistochemical expression of programmed death-ligand 1 and CD8 in glioblastomas: Dina Mohamed El Samman, Manal Mohamed El Mahdy, Hala Sobhy Cousha, Zeinab Abd El Rahman Kamar, Khaled Abdel Karim Mohamed, Hoda Hassan Abou Gabal; J Pathol Transl Med. 2021;55(6):388-397. Published online October 14, 2021; DOI: https://doi.org/10.4132/jptm.2021.08.04

3,066 View
149 Download
3 Web of Science
3 Crossref

Background
Glioblastoma is the most aggressive primary malignant brain tumor in adults and is characterized by poor prognosis. Immune evasion occurs via programmed death-ligand 1 (PD-L1)/programmed death receptor 1 (PD-1) interaction. Some malignant tumors have responded to PD-L1/PD-1 blockade treatment strategies, and PD-L1 has been described as a potential predictive biomarker. This study discussed the expression of PD-L1 and CD8 in glioblastomas.
Methods
Thirty cases of glioblastoma were stained immunohistochemically for PD-L1 and CD8, where PD-L1 expression in glioblastoma tumor tissue above 1% is considered positive and CD-8 is expressed in tumor infiltrating lymphocytes. The expression of each marker was correlated with clinicopathologic parameters. Survival analysis was conducted to correlate progression-free survival (PFS) and overall survival (OS) with PD-L1 and CD8 expression.
Results
Diffuse/fibrillary PD-L1 was expressed in all cases (mean expression, 57.6%), whereas membranous PD-L1 was expressed in six of 30 cases. CD8-positive tumor-infiltrating lymphocytes (CD8⁺ TILs) had a median expression of 10%. PD-L1 and CD8 were positively correlated (p = .001). High PD-L1 expression was associated with worse PFS and OS (p = .026 and p = .001, respectively). Correlation of CD8⁺ TILs percentage with age, sex, tumor site, laterality, and outcomes were statistically insignificant. Multivariate analysis revealed that PD-L1 was the only independent factor that affected prognosis.
Conclusions
PD-L1 expression in patients with glioblastoma is robust; higher PD-L1 expression is associated with lower CD8+ TIL expression and worse prognosis.

Citations

Citations to this article as recorded by

Tumor-associated microenvironment, PD-L1 expression and their relationship with immunotherapy in glioblastoma, IDH-wild type: A comprehensive review with emphasis on the implications for neuropathologists
Giuseppe Broggi, Giuseppe Angelico, Jessica Farina, Giordana Tinnirello, Valeria Barresi, Magda Zanelli, Andrea Palicelli, Francesco Certo, Giuseppe Barbagallo, Gaetano Magro, Rosario Caltabiano
Pathology - Research and Practice.2024; 254: 155144. CrossRef
Analysis of PD-L1 and CD3 Expression in Glioblastoma Patients and Correlation with Outcome: A Single Center Report
Navid Sobhani, Victoria Bouchè, Giovanni Aldegheri, Andrea Rocca, Alberto D’Angelo, Fabiola Giudici, Cristina Bottin, Carmine Antonio Donofrio, Maurizio Pinamonti, Benvenuto Ferrari, Stefano Panni, Marika Cominetti, Jahard Aliaga, Marco Ungari, Antonio Fi
Biomedicines.2023; 11(2): 311. CrossRef
Immuno-PET Imaging of Tumour PD-L1 Expression in Glioblastoma
Gitanjali Sharma, Marta C. Braga, Chiara Da Pieve, Wojciech Szopa, Tatjana Starzetz, Karl H. Plate, Wojciech Kaspera, Gabriela Kramer-Marek
Cancers.2023; 15(12): 3131. CrossRef

The Major Role of NF-κB in the Depth of Invasion on Acral Melanoma by Decreasing CD8⁺ T Cells: Hermin Aminah Usman, Bethy S. Hernowo, Maringan Diapari Lumban Tobing, Reti Hindritiani; J Pathol Transl Med. 2018;52(3):164-170. Published online April 20, 2018; DOI: https://doi.org/10.4132/jptm.2018.04.04

5,544 View
113 Download
6 Web of Science
6 Crossref

Abstract PDF

Background
The tumor microenvironment including immune surveillance affects malignant melanoma (MM) behavior. Nuclear factor κB (NF-κB) stimulates the transcription of various genes in the nucleus and plays a role in the inflammatory process and in tumorigenesis. CD8+ T cells have cytotoxic properties important in the elimination of tumors. However, inhibitory receptors on the cell surface will bind to programmed death-ligand 1 (PD-L1), causing CD8+ T cells to lose their ability to initiate an immune response. This study analyzed the association of NF-κB and PD-L1 expression levels and CD8+ T-cell counts with depth of invasion of acral MM, which may be a predictor of aggressiveness related to an increased risk of metastasis.
Methods
A retrospective cross-sectional study was conducted in the Department of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin Hospital using 96 cases of acral melanoma. Immunohistochemical staining was performed on paraffin blocks using anti–NF-κB, –PD-L1, and -CD8 antibodies and invasion depth was measured using dotSlide-imaging software.
Results
The study showed significant associations between the individual expression of NF-κB and PD-L1 and CD8+ T-cell number, with MM invasion depth. NF-κB was found to be a confounding variable of CD8+ T-cell number (p < .05), but not for PD-L1 expression (p = .154). Through multivariate analysis it was found that NF-κB had the greatest association with the depth of invasion (p < .001), whereas PD-L1 was unrelated to the depth of invasion because it depends on the number of CD8+ T cells (p = .870).
Conclusions
NF-κB plays a major role in acral MM invasion, by decreasing the number of CD8+ T cells in acral MM.

Citations

Citations to this article as recorded by

Possible Mechanisms of Oxidative Stress-Induced Skin Cellular Senescence, Inflammation, and Cancer and the Therapeutic Potential of Plant Polyphenols
Hui-Min Liu, Ming-Yan Cheng, Meng-Han Xun, Zhi-Wei Zhao, Yun Zhang, Wei Tang, Jun Cheng, Jia Ni, Wei Wang
International Journal of Molecular Sciences.2023; 24(4): 3755. CrossRef
Clinical features, molecular pathology, and immune microenvironmental characteristics of acral melanoma
Jianping Gui, Zhen Guo, Di Wu
Journal of Translational Medicine.2022;[Epub] CrossRef
High Expression of COX-2 Associated with the Depth of Invasion on Acral Melanoma by Increasing TGF-β1
Nastassa Gipsyianti, Afiati Aziz, Bethy S Hernowo, Hermin A Usman
Clinical, Cosmetic and Investigational Dermatology.2021; Volume 14: 209. CrossRef
More than just acral melanoma: the controversies of defining the disease
Sara S Bernardes, Ingrid Ferreira, David E Elder, Aretha B Nobre, Héctor Martínez‐Said, David J Adams, Carla Daniela Robles‐Espinoza, Patricia A Possik
The Journal of Pathology: Clinical Research.2021; 7(6): 531. CrossRef
CD103+ T Lymphocyte Count Linked to the Thickness of Invasion on Acral Melanoma without E-Cadherin Involvement
Fauzan Ali Zainal Abidin, Hermin Aminah Usman, Sri Suryanti, Bethy S Hernowo
Clinical, Cosmetic and Investigational Dermatology.2021; Volume 14: 1783. CrossRef
Translational pathology, genomics and the development of systemic therapies for acral melanoma
Yian Ann Chen, Jamie K. Teer, Zeynep Eroglu, Jheng-Yu Wu, John M. Koomen, Florian A. Karreth, Jane L. Messina, Keiran S.M. Smalley
Seminars in Cancer Biology.2020; 61: 149. CrossRef

Progressive Increase of Regulatory T Cells and Decrease of CD8+ T Cells and CD8+ T Cells/Regulatory T Cells Ratio during Colorectal Cancer Development: Tae Jung Jang; Korean J Pathol. 2013;47(5):443-451. Published online October 25, 2013; DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.5.443

7,832 View
52 Download
24 Crossref

Abstract PDF

Background

We examined the distribution of CD8+ T cells and regulatory T cells (Tregs), measured the CD8+ T cell/Tregs ratio, investigated the relationship between Tregs and cyclooxygenase-2 (COX-2) expression during colorectal cancer (CRC) development.

Methods

We performed immunohistochemical staining for CD8, forkhead box P3, E-cadherin, and COX-2 in 32 cases of invasive CRC, 10 cases of intramucosal CRC, 27 cases of high-grade tubular adenoma, 22 cases of low-grade tubular adenoma, and 32 cases of non-neoplastic conditions.

Results

We observed a progressive increase in Tregs, and a decrease in CD8+ T cells and the CD8+ T cells/Tregs ratio during CRC development. The alterations were most severe in high-grade tubular adenoma and CRC. COX-2 expression was positively associated with Tregs infiltration. The degree of T cell infiltration differed among tumor compartment and the ratio in the tumor center was the lowest of all areas. The ratio and number of CD8+ T cells in the tumor center and the invasive front of invasive CRC were associated with gender, differentiation, node metastasis and tumor budding.

Conclusions

Alteration in the distribution of both CD8+T cells and Tregs may contribute to the generation of an immune environment suitable for the development and progression of CRC.

Citations

Citations to this article as recorded by

Tumor Microenvironment and Immune Response in Lip Cancer
Anastasia G. Gkegka, Michael I. Koukourakis, Maria Lambropoulou, Alexandra Giatromanolaki
Cancers.2023; 15(5): 1478. CrossRef
Regenerating gene 4 promotes chemoresistance of colorectal cancer by affecting lipid droplet synthesis and assembly
Cong-Yu Zhang, Rui Zhang, Li Zhang, Zi-Mo Wang, Hong-Zhi Sun, Zheng-Guo Cui, Hua-Chuan Zheng
World Journal of Gastroenterology.2023; 29(35): 5104. CrossRef
Molecular mechanisms of tumour budding and its association with microenvironment in colorectal cancer
Phimmada Hatthakarnkul, Jean A. Quinn, Aula Ammar, Gerard Lynch, Hester Van Wyk, Donald C. McMillan, Chanitra Thuwajit, Joanne Edwards
Clinical Science.2022; 136(8): 521. CrossRef
Attackers and defenders: tumor buds and lymphocytes as morphological biomarkers in colorectal cancer
Sonay Kus Öztürk, Tariq S. Haddad, Inti Zlobec, Alessandro Lugli, Iris D. Nagtegaal
Diagnostic Histopathology.2022; 28(11): 480. CrossRef
The prognostic impact of the immune microenvironment in small-cell neuroendocrine carcinoma of the uterine cervix: PD-L1 and immune cell subtypes
Xiaoying Sun, Lili Liu, Ting Wan, Qidan Huang, Jieping Chen, Rongzhen Luo, Jihong Liu
Cancer Cell International.2022;[Epub] CrossRef
Preinvasive Colorectal Lesions of African Americans Display an Immunosuppressive Signature Compared to Caucasian Americans
Kristin Wallace, Georges J. Nahhas, Christine Bookhout, David N. Lewin, Chrystal M. Paulos, Nana Nikolaishvili-Feinberg, Stephanie M. Cohen, Silvia Guglietta, Ali Bakhtiari, E. Ramsay Camp, Elizabeth G. Hill, John A. Baron, Jennifer D. Wu, Alexander V. Al
Frontiers in Oncology.2021;[Epub] CrossRef
Cancer Immunoprevention: Current Status and Future Directions
Mahsa Keshavarz-Fathi, Nima Rezaei
Archivum Immunologiae et Therapiae Experimentalis.2021;[Epub] CrossRef
Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer
Qin Hong, Bing Li, Xiumei Cai, Zhengtao Lv, Shilun Cai, Yunshi Zhong, Bo Wen
Frontiers in Oncology.2021;[Epub] CrossRef
Immune Responses Vary in Preinvasive Colorectal Lesions by Tumor Location and Histology
Kristin Wallace, Georges J. El Nahas, Christine Bookhout, Jessica E. Thaxton, David N. Lewin, Nana Nikolaishvili-Feinberg, Stephanie M. Cohen, J. Grant Brazeal, Elizabeth G. Hill, Jennifer D. Wu, John A. Baron, Alexander V. Alekseyenko
Cancer Prevention Research.2021; 14(9): 885. CrossRef
CD103+ T Lymphocyte Count Linked to the Thickness of Invasion on Acral Melanoma without E-Cadherin Involvement
Fauzan Ali Zainal Abidin, Hermin Aminah Usman, Sri Suryanti, Bethy S Hernowo
Clinical, Cosmetic and Investigational Dermatology.2021; Volume 14: 1783. CrossRef
FOXP3 and CD25 double staining antibody cocktails identify regulatory T cells in different types of tumor tissues using tissue microarrays
Xinmin Liu, Xiaohong Wang, Jianmin Ding, Yan Gao, Yiming Zhao, Ruihua Zhao, Qigang Sun, Songlin Zhang
Annals of Diagnostic Pathology.2019; 38: 67. CrossRef
Epithelial CD80 promotes immune surveillance of colonic preneoplastic lesions and its expression is increased by oxidative stress through STAT3 in colon cancer cells
Chiara Marchiori, Melania Scarpa, Andromachi Kotsafti, Susan Morgan, Matteo Fassan, Vincenza Guzzardo, Andrea Porzionato, Imerio Angriman, Cesare Ruffolo, Stefania Sut, Stefano Dall’Acqua, Romeo Bardini, Raffaele De Caro, Carlo Castoro, Marco Scarpa, Igna
Journal of Experimental & Clinical Cancer Research.2019;[Epub] CrossRef
Automated Analysis of Lymphocytic Infiltration, Tumor Budding, and Their Spatial Relationship Improves Prognostic Accuracy in Colorectal Cancer
Ines P. Nearchou, Kate Lillard, Christos G. Gavriel, Hideki Ueno, David J. Harrison, Peter D. Caie
Cancer Immunology Research.2019; 7(4): 609. CrossRef
Colorectal cancer prevention: Immune modulation taking the stage
Rochelle Fletcher, Yi-Jun Wang, Robert E. Schoen, Olivera J. Finn, Jian Yu, Lin Zhang
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer.2018; 1869(2): 138. CrossRef
The Major Role of NF-κB in the Depth of Invasion on Acral Melanoma by Decreasing CD8+ T Cells
Hermin Aminah Usman, Bethy S. Hernowo, Maringan Diapari Lumban Tobing, Reti Hindritiani
Journal of Pathology and Translational Medicine.2018; 52(3): 164. CrossRef
Increased number of arginase 1-positive cells in the stroma of carcinomas compared to precursor lesions and nonneoplastic tissues
Tae Jung Jang, Sun A. Kim, Min Kyung Kim
Pathology - Research and Practice.2018; 214(8): 1179. CrossRef
Hematopoietic stem cell specific V-ATPase controls breast cancer progression and metastasis via cytotoxic T cells
Manoranjan Sahoo, Gajendra K. Katara, Mahmood Y. Bilal, Safaa A. Ibrahim, Arpita Kulshrestha, Sara Fleetwood, Kimiko Suzue, Kenneth D. Beaman
Oncotarget.2018; 9(69): 33215. CrossRef
Morphoproteomic-Guided Host-Directed Therapy for Tuberculosis
Robert E. Brown, Robert L. Hunter, Shen-An Hwang
Frontiers in Immunology.2017;[Epub] CrossRef
O-GlcNAcylation is associated with the development and progression of gastric carcinoma
Tae Jung Jang, Ui Jung Kim
Pathology - Research and Practice.2016; 212(7): 622. CrossRef
The Immune Landscapes of Polypoid and Nonpolypoid Precancerous Colorectal Lesions
Antonella Maglietta, Rosalia Maglietta, Teresa Staiano, Ramona Bertoni, Nicola Ancona, Giancarlo Marra, Leonardo Resta, Hiroshi Shiku
PLOS ONE.2016; 11(7): e0159373. CrossRef
Analysis of CD8+ Treg cells in patients with ovarian cancer: a possible mechanism for immune impairment
Shuping Zhang, Xing Ke, Suyun Zeng, Meng Wu, Jianfang Lou, Lei Wu, Peijun Huang, Lei Huang, Fang Wang, Shiyang Pan
Cellular & Molecular Immunology.2015; 12(5): 580. CrossRef
The Distribution of CD8- and Foxp3-positive T Cells in Skin Squamous Cell Tumors and Basal Cell Carcinomas
Tae Jung Jang
Journal of Life Science.2015; 25(6): 686. CrossRef
Correlation between infiltration of FOXP3+ regulatory T cells and expression of B7-H1 in the tumor tissues of gastric cancer
Jingying Hou, Zhong Yu, Rengyun Xiang, Chuqiang Li, Lin Wang, Shufen Chen, Qingyun Li, Mei Chen, Linyun Wang
Experimental and Molecular Pathology.2014; 96(3): 284. CrossRef
Methionine enkephalin (MENK) improves lymphocyte subpopulations in human peripheral blood of 50 cancer patients by inhibiting regulatory T cells (Tregs)
Qiushi Wang, Xinghua Gao, Zhe Yuan, Zhe Wang, Yiming Meng, Yan Cao, Nicolas P Plotnikoff, Noreen Griffin, Fengping Shan
Human Vaccines & Immunotherapeutics.2014; 10(7): 1836. CrossRef

First
Prev
Page of 1
Next
Last

Search