Abstract

A comparision of analytical methods for the determination of serum triglyceride was made between enzymatic and acetylacetone methods. The authors evaluated intra- and inter-assay variation as well as correlation between two methods. The results are as follows. 1. The mean values of triglyceride of one commercial control serum (Monitrol I) were 98. 02mg/dl by enzymatic method and 68.54mg/dl by acetylacetone method: enzymatic method shown higher value compared to acetylacetone method by 30mg/dl. 2. Intra and inter-assay variation (n=12) represented by coefficient of variation was 3.1% and 5.05% by enzymatic method, and 7.7% and 15.86 enzymatic method shorts lower inter-and inter assay variation. 3. A correlation between two methods (n=30) was made: correlation coefficient r=0.982, and linear regression y= 1.22x+9.32 4. Enzymatic method has the advantage of simplicity, rapidity and specificity.