Abstract

The effects of fractionated CCl4 pretreatments on the hepatotoxicity of CCl4 were studied. Rats were pretreated twice with subcutaneous injections of CCl4 (0.05m1/100g, 0.1m1/100g) and saline (0.1-0.2m1/100g). At 72 hours after pretreaments, 0.2m1/100g of CCl4 was given intraperitoneally, and the animals were sacrificed 24 hours later. Functional and morphological alterations of the liver were as follows. 1) Pretreatnlents of rats with CCl4 (especially in 0.1m1/100g) results in a significant increase in SGOT, SGPT and Alk-Pase level, and a decrease in serum cholesterol level. 2) Rats pretreated with CCl4 showed more extensive fatty degeneration and necrosis of the hepatocytes and PAS-positive granules in the tepatocytes were decreased more intensively than in saline-pretreated rats. 3) In the CCl4-pretreated rats(especially in 0.1m1/100g), Acid-PaBe activity was markedly increased, but Alk-Pase activity was decreased in the hepatic tissue. There was any difference in ATPase activity of the hepatocytes were not observed in each group of rats.