Analyses of clinical laboratory data of serum calcium, zinc, magnesium and copper were performed for the estimation of normal ranges by the Bhattacharya's method. The normal ranges of serum calcium, zinc, magnesium and copper are 8.5∼10.9 mg/100 ml, 27.7∼160.5㎍/100ml, 0.9∼2.5 mEq/l and 64.1∼174.5㎍/100ml respectively by atomic, absorption spectrophotometry. The normal ranges estimated are slightly wider than those from text but mean values are included in the reference ranges with good agreements.