Vaginitis in children is common, but there is a dearth of literature dealing with this problems. It is important to realize that vaginal mucosa of children is thin and neutral in pH, closely proximate to anus and urethra and poor in hygienic status of surrounding area. Most cases of vaginitis in children are considered nonspecific since no obvious etiologic agent can be proven. This report presents two cases of vaginitis due to Shigella flexneri. Both cases showed no diarrhea and urinary Problems. The organisms showed alkaline slant, acid butt on KIA, mannitol positive, indole negative, no H2S and no mobility. Slide agglutination test revealed complete agglutination with polyvalent B antiserum.