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Min-Sik Kim 2 Articles
Prevalence of high-risk human papillomavirus and its genotype distribution in head and neck squamous cell carcinomas
Yuil Kim, Young-Hoon Joo, Min-Sik Kim, Youn Soo Lee
J Pathol Transl Med. 2020;54(5):411-418.   Published online July 21, 2020
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  • 133 Download
  • 10 Citations
AbstractAbstract PDF
High-risk (HR) human papillomavirus (HPV) is found in a subset of head and neck (HN) squamous cell carcinomas (SCCs). For oropharyngeal SCCs, HR HPV positivity is known to be associated with good prognosis, and a separate staging system for HPV-associated carcinomas using p16 immunohistochemistry (IHC) as a surrogate test has been adopted in the 8th American Joint Committee on Cancer staging system. We examined the HR HPV status and the genotype distribution in five HN subsites.
Formalin-fixed paraffin-embedded tissue sections were used for p16 IHC and DNA extraction. HPV DNA detection and genotyping were done employing either a DNA chip-based or real-time polymerase chain reaction–based method.
During 2011–2019, a total of 466 SCCs were tested for HPV DNA with 34.1% positivity for HR HPV. Among HN subsites, the oropharynx showed the highest HR HPV prevalence (149/205, 75.1%), followed by the sinonasal tract (3/14, 21.4%), larynx (5/43, 11.6%), hypopharynx (1/38, 2.6%), and oral cavity (1/166, 0.6%). The most common HPV genotype was HPV16 (84.3%) followed by HPV35 (6.9%) and HPV33 (4.4%). Compared with HR HPV status, the sensitivity and specificity of p16 IHC were 98.6% and 94.3% for the oropharynx, and 99.2% and 93.8% for the tonsil, respectively.
Using a Korean dataset, we confirmed that HR HPV is most frequently detected in oropharyngeal SCCs. p16 positivity showed a good concordance with HR HPV DNA for oropharyngeal and especially tonsillar carcinomas. The use of p16 IHC may further be extended to predict HR HPV positivity in sinonasal tract SCCs.


Citations to this article as recorded by  
  • Practical Application of Circulating Tumor-Related DNA of Human Papillomavirus in Liquid Biopsy to Evaluate the Molecular Response in Patients with Oropharyngeal Cancer
    Agnieszka M. Mazurek, Tomasz W. Rutkowski
    Cancers.2023; 15(4): 1047.     CrossRef
  • The Prevalence of HPV in Oral Cavity Squamous Cell Carcinoma
    Seyed Keybud Katirachi, Mathias Peter Grønlund, Kathrine Kronberg Jakobsen, Christian Grønhøj, Christian von Buchwald
    Viruses.2023; 15(2): 451.     CrossRef
  • Positive Rate of Human Papillomavirus and Its Trend in Head and Neck Cancer in South Korea
    Hyun Woong Jun, Yong Bae Ji, Chang Myeon Song, Jae Kyung Myung, Hae Jin Park, Kyung Tae
    Frontiers in Surgery.2022;[Epub]     CrossRef
  • Transcriptionally active HPV in OPMD and OSCC: A systematic review following the CAP/ASCO guidelines
    Laura Borges Kirschnick, Lauren Frenzel Schuch, Maria Eduarda Pérez‐de‐Oliveira, Ana Gabriela Costa Normando, Bruno Augusto Linhares Almeida Mariz, Eliete Neves Silva Guerra, Felipe Martins Silveira, Ana Carolina Uchoa Vasconcelos, Luciana Estevam Simonat
    Oral Diseases.2022; 28(8): 2309.     CrossRef
  • High-risk HPV Does not Appear to be an Important Risk Factor for Sinonasal Carcinomas in Turkish Population: A Tertiary Center Experience
    Evsen Apaydin Arikan, Levent Aydemir, Murat Ulusan, Dilek Yilmazbayhan, Yasemin Ozluk
    International Journal of Surgical Pathology.2022; : 106689692210915.     CrossRef
  • Effect of National Oral Health Screening Program on the Risk of Head and Neck Cancer: A Korean National Population-Based
    Chan Woo Wee, Hyo-Jung Lee, Jae-Ryun Lee, Hyejin Lee, Min-Jeong Kwoen, Woo-Jin Jeong, Keun-Yong Eom
    Cancer Research and Treatment.2022; 54(3): 709.     CrossRef
  • Expression of p16, p53, and TLR9 in HPV-Associated Head and Neck Squamous Cell Carcinoma: Clinicopathological Correlations and Potential Prognostic Significance
    Shu Wang, Xibing Zhuang, Caixia Gao, Tiankui Qiao
    OncoTargets and Therapy.2021; Volume 14: 867.     CrossRef
  • The Role of Human Papilloma Virus in Dictating Outcomes in Head and Neck Squamous Cell Carcinoma
    Shane Brennan, Anne-Marie Baird, Esther O’Regan, Orla Sheils
    Frontiers in Molecular Biosciences.2021;[Epub]     CrossRef
  • A Contemporary Systematic Review on Repartition of HPV-Positivity in Oropharyngeal Cancer Worldwide
    Amanda F. Carlander, Kathrine K. Jakobsen, Simone K. Bendtsen, Martin Garset-Zamani, Charlotte D. Lynggaard, Jakob Schmidt Jensen, Christian Grønhøj, Christian von Buchwald
    Viruses.2021; 13(7): 1326.     CrossRef
  • The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland
    Joanna Katarzyna Strzelczyk, Krzysztof Biernacki, Jadwiga Gaździcka, Elżbieta Chełmecka, Katarzyna Miśkiewicz-Orczyk, Natalia Zięba, Janusz Strzelczyk, Maciej Misiołek
    Diagnostics.2021; 11(12): 2180.     CrossRef
Expression of Human Papillomavirus-Related Proteins and Its Clinical Implication in Tonsillar Squamous Cell Carcinoma
Joon Seon Song, Min-Sik Kim, Joon Wook Park, Youn Soo Lee, Chang Suk Kang
Korean J Pathol. 2012;46(2):177-186.   Published online April 25, 2012
  • 7,988 View
  • 36 Download
  • 9 Citations
AbstractAbstract PDF

Human papillomavirus (HPV) is known to cause of oropharyngeal squamous cell carcinoma (SqCC). HPV positive SqCCs overexpress p16 and are associated with better survival. Several markers of cell cycles and apoptosis have been reported as a prognostic value. We examined the prognostic value of HPV status, p16, cyclin D1, and Bcl-2 in patients with tonsillar SqCC.


Tissue microarrays were constructed in 56 cases of tonsillar SqCC for which we performed an immunohistochemistry and an in situ hybridization (ISH) of the HPV.


Of the 56 cases, 31 (55.3%) were positive for p16 and 20 (35.7%) were positive for HPV ISH. The expressions of p16, cyclin D1, and Bcl-2 were not correlated with the clinicopathologic variables including smoking status, differentiation and pT- and pN-stages. The HPV ISH positive group showed a better overall survival than the HPV negative group (p=0.04), and the p16 positive group showed a better disease free survival (DFS) than the negative group (p=0.016). Cox regression analysis showed that only p16 positivity was an independent prognostic factor for DFS (p=0.03; hazard ratio, 10.1).


Our results indicate that both p16 expression and HPV status are useful indicators for risk stratification in patients with tonsillar SqCC.


Citations to this article as recorded by  
  • Positive Rate of Human Papillomavirus and Its Trend in Head and Neck Cancer in South Korea
    Hyun Woong Jun, Yong Bae Ji, Chang Myeon Song, Jae Kyung Myung, Hae Jin Park, Kyung Tae
    Frontiers in Surgery.2022;[Epub]     CrossRef
  • Negative Prognostic Implication of TERT Promoter Mutations in Human Papillomavirus–Negative Tonsillar Squamous Cell Carcinoma Under the New 8th AJCC Staging System
    Hyunchul Kim, Mi Jung Kwon, Bumjung Park, Hyo Geun Choi, Eun Sook Nam, Seong Jin Cho, Kyueng-Whan Min, Eun Soo Kim, Hee Sung Hwang, Mineui Hong, Taeryool Koo, Hyo Jung Kim
    Indian Journal of Surgical Oncology.2021; 12(S1): 134.     CrossRef
  • In situ hybridization for high risk HPV E6/E7 mRNA in oropharyngeal squamous cell carcinoma
    Krish Suresh, Parth V. Shah, Sydney Coates, Borislav A. Alexiev, Sandeep Samant
    American Journal of Otolaryngology.2021; 42(1): 102782.     CrossRef
  • Prevalence of high-risk human papillomavirus and its genotype distribution in head and neck squamous cell carcinomas
    Yuil Kim, Young-Hoon Joo, Min-Sik Kim, Youn Soo Lee
    Journal of Pathology and Translational Medicine.2020; 54(5): 411.     CrossRef
  • Human Papillomavirus Testing in Head and Neck Carcinomas: Guideline From the College of American Pathologists
    James S. Lewis, Beth Beadle, Justin A. Bishop, Rebecca D. Chernock, Carol Colasacco, Christina Lacchetti, Joel Todd Moncur, James W. Rocco, Mary R. Schwartz, Raja R. Seethala, Nicole E. Thomas, William H. Westra, William C. Faquin
    Archives of Pathology & Laboratory Medicine.2018; 142(5): 559.     CrossRef
  • Detection of HPV infection in head and neck cancers: Promise and pitfalls in the last ten years: A meta-analysis
    Carolin G�tz, Clara Bischof, Klaus-Dietrich Wolff, Andreas Kolk
    Molecular and Clinical Oncology.2018;[Epub]     CrossRef
  • Frequent hepatocyte growth factor overexpression and low frequency of c-Met gene amplification in human papillomavirus–negative tonsillar squamous cell carcinoma and their prognostic significances
    Mi Jung Kwon, Dong Hoon Kim, Hye-Rim Park, Hyung Sik Shin, Ji Hyun Kwon, Dong Jin Lee, Jin Hwan Kim, Seong Jin Cho, Eun Sook Nam
    Human Pathology.2014; 45(7): 1327.     CrossRef
  • Human papillomavirus-stratified analysis of the prognostic role of miR-21 in oral cavity and oropharyngeal squamous cell carcinoma
    Yoon Ho Ko, Hye Sung Won, Der Sheng Sun, Ho Jung An, Eun Kyoung Jeon, Min Sik Kim, Han Hong Lee, Jin Hyoung Kang, Chan Kwon Jung
    Pathology International.2014; 64(10): 499.     CrossRef
  • Human Papillomavirus Prevalence and Cell Cycle Related Protein Expression in Tonsillar Squamous Cell Carcinomas of Korean Patients with Clinicopathologic Analysis
    Miji Lee, Sung Bae Kim, Sang-wook Lee, Jong-Lyel Roh, Seung-Ho Choi, Soon Yuhl Nam, Sang Yoon Kim, Kyung-Ja Cho
    Korean Journal of Pathology.2013; 47(2): 148.     CrossRef

JPTM : Journal of Pathology and Translational Medicine