Human Herpesvirus 8-Negative and Epstein-Barr Virus-Positive Effusion-Based Lymphoma in a Patient with Human Immunodeficiency Virus

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Abstract

CASE REPORT

A 39-year-old man visited our hospital because of two enlarging dusky-colored patches on his chin and forehead, both of which appeared 1 month prior to his visit. Both skin lesions were diagnosed as Kaposi sarcoma, and the patient was diagnosed to be HIV positive. Two months later, he experienced sudden onset of chest pain and difficulty breathing. Laboratory tests showed high levels of white blood cells (10,600/µL) with 73.5% granulocytes and serum lactate dehydrogenase of 679 IU/L. The patient’s CD4 T-cell count had decreased to 19 cells/µL. Hepatitis A, B, or C viruses were not detected. Computed tomographic scans of his chest revealed a 5.8-cm emphysematous bulla in the right middle lobe of his lung, while the left lobe showed diffuse ground glass opacities, suggestive of interstitial pneumonia (Fig. 1A). The patient underwent wedge resection of the lung, which grossly revealed a multilocular bullous cavity surrounded by whitish pneumonic consolidation (Fig. 1B). Microscopically, aggregates of atypical lymphoid cells were floating in the bullous cavity without forming solid masses. These cells showed medium to large pleomorphic nuclei with prominent nucleoli and basophilic cytoplasm, revealing plasmablastic features (Fig. 2). Through immunohistochemistry, tumor cells were found to exhibit a non-germinal center B-cell nature showing positivity for CD20, CD79a, and MUM1 but negativity for CD3, CD10, BCL6, CD30, and CD138. The Ki-67 labeling index reached up to 80%–90%. Tumor cells were positive for EBV-encoded RNA in situ hybridization, EBV latent membrane protein-1 (LMP1), EBV nuclear antigen-2 (EBNA-2), but negative for HHV8. The background bullous cavity and adjacent alveolar cavities were filled with frothy exudates containing Pneumocystis jirovecii clearly visible after application of Gomori methenamine silver stain. Therefore, the patient was diagnosed with EBL, not PEL, arising in the PJP-associated cavitary lung lesion. He was treated with highly active antiretroviral therapy along with anti-microbes for 6 months, followed by chemotherapy.

Fig. 1.

A computed tomographic scan (A) and serial lung sections (B) displaying a 5.8-cm multilocular emphysematous bulla surrounded by pneumonic consolidation in the right middle lobe of the lung. The left lung shows diffuse ground glass opacities, which is consistent with interstitial pneumonia.

Fig. 2.

Lymphoma cells have large eccentric pleomorphic nuclei and basophilic cytoplasm, showing plasmablastic features (A). Proliferating and necrotic tumor cells (arrows) are located in the bullous cavity, representing effusion-based nature (A inset). Tumor cells are positive for CD20 immunohistochemistry (IHC) (B), Epstein-Barr virus (EBV) in situ hybridization (C) and also EBV nuclear antigen-2 IHC (D).

DISCUSSION

This is a unique case of EBL occurring in the PJP-associated bullous cavity of the lung. The differential diagnosis of this case includes HHV8-negative EBL, PEL, and diffuse large B-cell lymphoma (DLBCL) associated with chronic inflammation (Table 1). While PEL is the only subtype of EBL listed in the 2008 WHO classification, HHV8-negative EBL has been steadily considered a separate entity, because the demographics, immunophenotype, and clinical outcome of HHV8-negative EBL are inconsistent with PEL [3,4]. In contrast to this case, PEL is invariably positive for HHV8, and usually negative for pan-B-cell markers [1]. Compared to HHV8-negative EBL, this case did not show any clinical condition of fluid overload such as cirrhosis, protein-losing enteropathy, or cardiac disease. In addition, the tumor in this case developed in the setting of both HIV and EBV positivity, while negative for the hepatitis C virus, which is the reverse of HHV8-negative EBL. With regard to the tumor’s origin, the present case is unique in that all previously reported cases of EBL involved pleural, peritoneal, or pericardial cavities, except for one, which was detected from scrotal effusion fluids [6]. Although our case most closely resembles DLBCL associated with chronic inflammation, in that it is a lymphoid neoplasm often involving the body cavity and occurring in a condition of chronic inflammation and EBV infection, DLBCL forms a detectable tumor mass and typically develops after a long-standing inflammation, usually over 10 years [7]. It is unclear whether this particular tumor should be considered a previously undescribed subtype of DLBCL, or whether it corresponds with a rare variant of HHV8-negative EBL or DLBCL associated with chronic inflammation.

Table 1.

Main differential diagnoses of this case

Interestingly, this tumor was accidentally discovered in the pneumothorax as a complication of PJP. The incidence of pneumothorax in HIV-positive patients is approximately 2%, but can reach up to 9% in those with PJP [8,9]. It has been reported that chronic inflammation and localized immunosuppression may enhance the clonal evolution of EBV-infected lymphocytes [10]. DLBCL cells in HIV-positive patients have been known to express the interleukin-6 (IL-6) receptor, which can respond to the IL-6 produced by adjacent non-neoplastic cells in a paracrine manner [11]. In addition, similar to DLBCL associated with chronic inflammation, the tumor cells in this case revealed EBV latency type III (LMP1-positive, EBNA-2–positive) infection, which represents the most complete form of latent gene expression [12,13]. This finding is in contrast to PEL, in which the tumor cells are infected with the more restricted EBV latency type II (LMP1-positive, EBNA-2–negative) infection [14].

In conclusion, the present case is a rare instance of HHV8-negative and EBV-positive EBL in an HIV-positive patient. This type of clinical presentation has never been described previously, and it should be kept in mind in order to diagnose occult lymphomas.

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