Hepatocellular Carcinoma Arising in a Huge Hepatocellular Adenoma with Bone Marrow Metaplasia

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J Pathol Transl Med. 2018;52(4):226-231

Publication date (electronic) : 2017 December 27

doi : https://doi.org/10.4132/jptm.2017.11.12

Hyo Jeong Kang^1,2, Hui Jeong Jeong¹, So-Woon Kim¹, Eunsil Yu^1,3, Young-Joo Lee⁴, So Yeon Kim⁵, Jihun Kim^,1

¹Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

²Department of Physiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

³Department of Asan Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

⁴Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

⁵Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea

Corresponding Author Jihun Kim, MD, PhD Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43 gil, Songpa-gu, Seoul 05505, Korea Tel: +82-2-3010-4556 Fax: +82-2-472-7898 E-mail: Jihunkim@amc.seoul.kr

Received 2017 September 8; Revised 2017 October 30; Accepted 2017 November 12.

Abstract

CASE REPORT

A 24-year-old woman was admitted to an outside hospital presenting with abdominal discomfort. She was referred to Asan Medical Center and was found to have a huge liver mass that was detected by abdominal ultrasonography. The diagnosis of pre-operative liver biopsy performed by an outside hospital was HCA. Her medical and family histories were unremarkable. She had never taken OCPs and had a body mass index of 21.48 kg/m². Hepatitis B surface (HBs) antigen, anti-HBs antibody, and anti–hepatitis C virus antibody were all negative. A complete blood count and serum chemistry tests, including coagulation and liver function tests, were normal except for elevated levels of gamma glutamyl transferase (111 IU/L; normal range, 8 to 61 IU/L). Serum tumor marker of prothrombin induced by vitamin K absence-II (PIVKA-II) levels were markedly elevated (2,964 mAU/mL; normal range, ≤ 40 mAU/mL). α-Fetoprotein levels were slightly elevated (11.1 ng/mL; normal range, ≤ 7.0 ng/mL), while those of serum carcinoembryonic antigen (0.9 ng/mL; normal range, ≤ 5 ng/mL) and carbohydrate antigen 19-9 (3.8 U/mL; normal range, ≤ 37 U/mL) were within normal limits. A dynamic liver and pelvis computerized tomography (CT) scan revealed a 20 cm hypervascular mass occupying segments 4, 5, and 8 of the liver (Fig. 1A). An enhanced CT image exhibited high attenuation during the arterial phase and decreasing attenuation during the portal and delayed phases. The lesion showed heterogeneous foci of hemorrhage, necrosis, fat, and calcification. An experienced radiologist (S.Y.K.) interpreted the lesion as a huge HCA and suspected a partly malignant transformation. The patient underwent segmentectomy and partial hepatectomy to remove the lesion. Macroscopically, the resected specimen was filled with a well circumscribed 19.9 × 18.4 × 7.9-cm solid mass, and its cut surface showed a heterogeneous yellow-brown color, multinodular growth, partly cystic degeneration, hemorrhage, and necrosis (Fig. 1B). Microscopically, the lesion exhibited typical features of a HCA and was characterized by well differentiated hepatocytes arranged in thin cords or sheets with bland cytology and abundant eosinophilic cytoplasm (Fig. 2A). The parenchyma showed dilated sinusoids, occasionally with large aberrant arteries, and multifocal inflammation composed of lymphocytes, neutrophils, and eosinophils (Fig. 2A, B). Immunohistochemical stains of HCA cells showed diffuse positivity of liver fatty acid binding protein, serum amyloid A (Fig. 2C), C-reactive protein (Fig. 2D), and glutamine synthetase (Fig. 2E) antibodies and partial nuclear positivity for β-catenin antibody (Fig. 2F), suggesting an inflammatory HCA with β-catenin activation. Although this case showed histologically typical features of inflammatory HCA without histological features of other subtypes, it coexisted with β-catenin nuclear positivity, suggesting a β-catenin–activated inflammatory HCA. It was difficult to grossly distinguish the area of malignant transformation. However, multiple malignant foci were found microscopically that occupied less than 10% of the total tumor area. These malignant foci were all well differentiated HCC that partially resembled the surrounding HCA but also showed nuclear hyperchromasia and pleomorphism and thickened trabeculae (Fig. 2G). Glutamine synthetase showed stronger expression in the HCC area than in the HCA area, and heat shock protein-70 (Fig. 2H) and glypican 3 (Fig. 2I) were positive in the HCC area. As is typical of HCC, reticulin loss (Fig. 2J) and diffuse CD34 expression on sinusoidal cells (Fig. 2K) were observed. The Ki-67 labeling index of the HCC area (15%) was significantly increased compared with that of the HCA area (< 2%) (Fig. 2L). Corresponding to visible calcification regions in the CT scan were areas of bone marrow metaplasia (Fig. 3A) that were characterized by mature lamellar bone forming trabeculae intermingled with fat tissue that contained myeloid and erythroid cells (Fig. 3B, C). The patient was discharged 10 days after surgery and showed no evidence of recurrence at the last follow up visit 23 months after surgery.

Fig. 1.

Imaging and gross pathological findings. (A) Dynamic liver and pelvis computed tomography shows an enlarged liver with a huge, well-defined, hypervascular mass in segments 4, 5, and 8 of the liver during arterial phase. (B) The surface of a slice from a surgically resected specimen reveals a heterogeneously yellow-brown color and multinodular growth with partly cystic degeneration that contained hemorrahge and necrosis.

Fig. 2.

Microscopic and immunohistochemical findings of hepatocellular adenoma (HCA), inflammatory type with β-catenin activation (A–F) and hepatocellular carcinoma (HCC) (G–L). (A) Dilated sinusoids and intratumoral inflammation in the portal tract-like areas are observed in the parenchyma. (B) Large aberrant arteries with infiltration of variable inflammatory cells including lymphocytes, neutrophils, and eosinophils are occasionally observed. Tumor cells show diffuse cytoplasmic expression of serum amyloid A (C), C-reactive protein (D), and glutamine synthetase (E). (F) Nuclear β-catenin expression (arrows) is observed in several tumor cells. (G) Nuclear atypia and thick trabeculae are observed in the HCC component. (H) Heat shock protein-70 is strongly and diffusely positive. (I) Glypican 3 is partially positive. (J) Loss of reticulin is observed in the HCC component, whereas reticulin staining is preserved in the HCA (inset). (K) CD34 expression on sinusoidal cells is strong and diffuse in HCC but is patchy in HCA (inset). (L) The Ki-67 labeling index of the HCC area is significantly increased compared with that of HCA (inset).

Fig. 3.

Histological findings of bone marrow metaplasia. (A) Bone marrow metaplasia is observed in the inner hepatocellular adenoma. (B, C) Bone marrow metaplasia is characterized by mature lamellar bone that formed trabeculae intermingled with fat tissue containing erythroblasts, myeloblasts, and megakaryocytes. (D) Tumor cells near the bone marrow metaplasia shows positivity for epithelial cell adhesion molecule immunohistochemical staining.

This study was approved by the Institutional Review Board of Asan Medical Center with a waiver of informed consent (IRB No.2017-0679).

DISCUSSION

HCA is an uncommon benign liver neoplasm composed of hepatocytes and typically develop in a non-cirrhotic liver. It primarily occurs in young to middle-aged women with a history of OCP use. Estrogen or exogenous androgen use for medical purposes is also a recognized risk factor for HCA. Additionally, rarely HCA is found in children and is association with other conditions such as glycogen storage disease, galactosemia, tyrosinemia, familial polyposis coli, Fanconi anemia, and hepatic iron overload with β-thalassemia [11,12]. It has recently been reported that the incidence of HCA is increasing in males and non-OCP individuals. Additionally, there is a trend towards increasing cases of multiple HCAs. This may primarily be a consequence of the higher prevalence of obesity and metabolic syndrome, rather than an effect of OCPs, which have been considered to be the main cause for the development of HCA [1,13,14]. Interestingly, the woman with HCA in this study had a huge mass at a young age, and she showed no history of OCP use or genetically determined metabolic disease.

Malignant transformation of HCA is the main reason for surgical treatment. It is important to identify factors that increase the risk of malignancy. However, HCA is difficult to distinguish from HCC because of its similar imaging characteristics and histopathological features [6]. Several studies have indicated that high risk groups of patients having an increased risk of malignant alteration from HCA display larger tumors (> 5 cm), have a history of androgen or anabolic steroid intake, are male, and are patients with glycogen storage disease [12,15-19]. HCA has recently been roughly classified into four subgroups according to genotype-phenotype based analysis. Moreover, β-catenin–activated HCA represents a well-known factor for an increased risk of malignant transformation compared with the other subtypes [17]. In our present case, although the patient was a young woman who had only abdominal discomfort without any symptoms related to malignancy the large tumor size and high level of PIVKA-II could predict the possibility of malignant transformation from HCA. Therefore, a rapid assessment of mass removal was performed to prevent intraperitoneal hemorrhage, rupture, malignant progression, or metastasis. Within the surgically resected specimen, partially malignant transformation was confirmed by histological and immunohistochemical examination. Nuclear accumulation of β-catenin antibody was detected in areas corresponding to malignant foci by immunohistochemistry, which was consistent with an association with malignant transformation in HCA.

A notable feature of this case was the presence of bone marrow metaplasia in the inner HCA. Bone or osteoid formation has been found in special types of primary liver tumors, especially hepatoblastoma. However, bone marrow metaplasia with hematopoietic cells is extremely rare in primary liver tumors in adults, especially in cases of HCA or HCC. Only four cases have been reported in the English literature in which areas of bone marrow metaplasia were found in HCC or HCA (Table 1). According to a review of the literature, this represents the first case report of HCC arising from HCA with complete bone marrow metaplasia consisting of both fat components and hematopoietic cells in an adult younger than 30 years old. It has been previously demonstrated that bone marrow-derived stem cells can differentiate into hepatic progenitor cells that may play a role in liver tumor development [10,20]. In this present case, hepatic progenitor cells with epithelial cell adhesion molecule expression (Fig. 3D) were observed around bone marrow metaplasia but were less frequent or not detected in areas far from bone marrow metaplasia.

Table 1.

Overview of cases of HCA and HCC with bone marrow metaplasia

In conclusion, we report a case of HCC arising from inflammatory HCA with β-catenin activation. Two important points of this case are that malignant transformation in huge HCA can occur in a young woman without risk factors such as use of OCP or genetic metabolic diseases and that complete bone marrow metaplasia can occur in HCA. This case report emphasizes that a large HCA can show malignant transformation, even in a young woman, and that these tumors should be promptly, surgically resected.

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