Fig. 1Immunohistochemical (IHC) stains for histone acetyltransferase 1 (A) and histone deacetylase (HDAC) 1 (B) show strong positive cells in the reactive germinal centers and are weak positive on the endothelial cells. IHC stains for HDAC2 (C) and HDAC3 (D) show more strong positive reaction for the histiocytes than for the endothelial cells.
Fig. 2Immunohistochemical (IHC) stain for histone deacetylase 1 (HDAC1) is negative (A). Immunohistochemistry for HDAC1 shows the same intensity (1+) on the lymphoma cells and the endothelial cells (B). IHC stains for HDAC1 are 2+ (C) and 3+ (D) on the lymphoma cells that are stronger than on the nearby endothelial cells.
Fig. 3The comparative histograms for malignant lymphomas including diffuse large B-cell lymphomas (DLBCL) (A), peripheral T-cell lymphomas, not otherwise specified (PTCL-NOS) (B), and NK/T-cell lymphomas, nasal type (NKTCL) (C) and reactive lymphoid hyperplasia (RLH) (D). HAT1, histone acetyltransferase 1; HDAC, histone deacetylase. blank; p>0.05.
Fig. 4A higher degree of expression of histone acetyltransferase 1 (HAT1) in cases of malignant lymphoma than those of reactive lymphoid hyperplasia (RLH). Following a comparison with the degree of the expression in cases of RLH, the degree of the expression of HAT1 is significantly higher in cases of diffuse large B-cell lymphomas (DLBLC) (3/4) or peripheral T-cell lymphomas, not otherwise specified (PTCL-NOS) (2/3). Western blot analysis of HAT1 (A). Anti-actin antibodies are used as a loading control. The normal degree of the expression of HAT1 (B). Asterisks indicate p<0.05. Statistical significance is analyzed using a t-test.
Fig. 5The degree of the expression of histone acetyltransferase 1 (HAT1) and histone deacetylase 1 (HDAC1) is correlated with a poor survival in cases of diffuse large B-cell lymphomas (DLBCL) (A, B) and those of peripheral T-cell lymphomas, not otherwise specified (PTCL-NOS) (C, D), respectively. The degree of the expression of HDAC3 is correlated with a good survival in cases of extranodal NK/T-cell lymphomas, nasal type (NKTCL) (E).
Table 1Antibodies used for this study
Table 2Summary of data of clinical cases of malignant lymphoma
Table 3Spearman correlation between the expressions of proteins