Primary Peripheral Gamma Delta T-Cell Lymphoma of the Central Nervous System: Report of a Case Involving the Intramedullary Spinal Cord and Presenting with Myelopathy

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J Pathol Transl Med. 2019;53(1):57-61

Publication date (electronic) : 2018 October 1

doi : https://doi.org/10.4132/jptm.2018.08.21

Jeemin Yim, Seung Geun Song, Sehui Kim, Jae Won Choi¹, Kyu-Chong Lee², Jeong Mo Bae, Yoon Kyung Jeon

Department of Pathology, Seoul National University Hospital, Seoul, Korea

¹Department of Radiology, Seoul National University Hospital, Seoul, Korea

²Department of Radiology, Korea University Guro Hospital, Seoul, Korea

Corresponding Author Yoon Kyung Jeon, MD, PhD Department of Pathology, Seoul National University College of Medicine, 103 Daehak-ro, Jongno-gu, Seoul 03080, Korea Tel: +82-2-740-8323 Fax: +82-2-743-5530 E-mail: ykjeon@snu.ac.kr

Received 2018 July 23; Revised 2018 August 20; Accepted 2018 August 21.

Abstract

CASE REPORT

A 75-year-old Korean woman presented with back pain and lower extremity weakness for 3.5 months. Lower extremity weakness causing difficulty in ambulation was temporarily relieved after steroid therapy. She had underlying hypertension, hyperlipidemia, and type 2 diabetes and had no history of immunodeficiency. Spine magnetic resonance imaging (MRI) revealed multiple enhancing intramedullary nodular lesions in the spinal cord at T9/10, T11, and L5 levels (Fig. 1A). Brain MRI revealed a small enhancing nodular lesion in the periventricular white matter of the left lateral ventricle (Fig. 1C–E). Clinicoradiological diagnoses included tumorous conditions such as lymphoma, glioma, and metastasis or nontumorous myelitis. Spinal and brain lesions had increased in size on MRI taken 1.5 months after initial presentation (Fig. 1B, F–J). Multiple newly developed enhancing nodules were observed in the lateral subependymal lining, left frontal lobe, and right temporal lobe (Fig. 1K). Positron emission tomography scan showed mild hypermetabolism in spinal cord lesions. No other abnormal findings were identified in the systemic organs and skin. The patient underwent T11 laminectomy and tumor removal. Microscopic examination of tumor revealed diffuse infiltration of monotonous, medium-to-large atypical lymphocytes with round nuclei, condensed chromatin, pale-to-eosinophilic cytoplasm, and small inconspicuous nucleoli (Fig. 2). Vasculature with high endothelial cells was noted throughout the tumor, and perivascular infiltration of tumor cells was occasionally observed along with diffuse infiltration of tumor cells in glial tissue (Fig. 2). Immunohistochemically, the atypical cells were CD3(+), CD20(–), TCRβF1(–), TCRγ(+), CD30(–), CD4(–), CD8(–), CD10(–), BCL6(–), MUM1(–), CD56(+), TIA-1(+), granzyme B(focal +), and CD103(+) (Fig. 3A–H). The Ki-67 index was about 80%, and Epstein-Barr virus in situ hybridization showed no positive cells. T-cell monoclonality was detected by TCRG gene rearrangement study using IdentiClone TCRG Gene Clonality Assay (Invivoscribe Technologies Inc., San Diego, CA, USA) (Fig. 3I). This case represents a unique PCNSL of γδ T-cell origin involving the spinal cord that presented with paraplegia.

Fig. 1.

Spine and brain magnetic resonance imaging (MRI) features at presentation (A, C–E) and 6 weeks later (B, F–K). (A) Spine MRI sagittal view revealed enhancing intramedullary nodular lesions at T9/10 and T11 levels (arrows). (B) Six weeks later, enhancing intramedullary nodular lesions (arrows) at T7/8, T9/10, and T11 levels were enlarged, and an enhancing nodule appeared at L5 level with leptomeningeal enhancement. (C–E) A nodular lesion (arrows) was observed in the periventricular white matter of the left lateral ventricle, which showed enhancement in T1 enhanced image (D) compared to T1 weighted image (C) and heterogeneous high signal intensity in T2 weighted images (E). (F–H) After 6 weeks, the lesion (arrows) increased in size with surrounding edema in T1 weighted (F), T1 enhanced (G), and T2 weighted images (H). (I, J ) Diffusion weighted images revealed diffusion restriction within the tumor with high signal intensity (arrow) (I) and corresponding low signal intensity (arrow) on the apparent diffuse coefficient map (J). (K) There were also multiple newly developed enhancing nodules in the lateral subependymal lining, left frontal lobe, and right temporal lobe (arrow).

Fig. 2.

Histologic features of γδ T-cell lymphoma involving the spinal cord. (A) Monomorphic medium-to-large atypical lymphoid cells diffusely infiltrating the spinal cord parenchyma with occasional perivascular arrangement. (B) Atypical lymphoid cells showed clear to eosinophilic cytoplasm with distinct cell borders and hyperchromatic nuclei with small indistinct nucleoli.

Fig. 3.

Immunohistochemical and genetic features of γδ T-cell lymphoma involving the spinal cord. Tumor cells were CD3(+) (A), TCRβF1(–) (B), TCRγ(+) (C), CD4(–) (D), CD8(–) (E), CD56(+) (F), TIA1(+) (G), and CD103(+) (H). (I) Monoclonal peak was observed in TCRγ gene rearrangement study.

The Institutional Review Board (IRB) of Seoul National University Hospital (SNUH) approved this study (No. H-1807-070-958) and waived the need for informed consent from patients.

DISCUSSION

The detailed pathological features of T-PCNSL remain unclear. In the largest series of T-PCNSL (n = 45) published 2005 by Shenkier et al. [2], tumor cells were “small or small-to-medium sized” in 12 cases and “pleomorphic or medium-to-large” in 13 cases. Of the nine Korean patients with T-PCNSL reported by Lim et al. [3], seven were diagnosed with PTCL, while two were diagnosed with T-lineage lymphoma with no further specification. Menon et al.’s series [4] (n = 18) of T-PCNSL comprised 15 cases of PTCL not otherwise specified (NOS) with small (n = 2), small-medium (n = 6), medium (n = 3), and medium-large or large (n = 4) tumor cells; one case was anaplastic lymphoma kinase (ALK) (+) ALCL and two cases were ALK(–) ALCLs. Of note, two of the 15 PTCL NOS cases expressed TCRγ, suggestive of γδ T-cell derivation. One of the patients with γδ T-PCNSL was a 31-year-old male with bilateral temporal lobe involvement. The other was a 56-year-old female with a solitary frontal mass. The tumors of both patients were composed of small-medium cells with CD4(–)CD8(+) phenotype [4]. Recently, Mooney et al. [10] reported another case of γδ T-PCNSL involving the cerebellum in a 26-year-old Korean female. To the best of our knowledge, our patient is the fourth case of γδ T-PCNSL and the first case of γδ T-PCNSL involving the spinal cord presenting with myelopathy.

The clinical features and outcomes of patients with T-PCNSL remain unclear. Based on previous reports, T-PCNSL predominantly involves older patients, but with a wide age range from 3 to 84 years, and the male to female ratio is 1.8:1. In prior reports, involvement of deep brain structures and presentation with multifocal lesions were observed in about 34% and 43% patients, respectively [2-4]. The disease-specific survival of patients with T-PCNSL was 25 months (95% confidence interval, 11 to 38 months) [2]. Although the prognosis of T-PCNSL is controversial, Shenkier et al. [2] and Lim et al. [3] demonstrated that the clinical outcome of patients with T-PCNSL was comparable to that of patients with B-PCNSL, and performance status and high-dose methotrexate-based therapy were associated with patient prognosis. Of the four reported cases with γδ T-PCNSL including our case, detailed treatment modality and outcome are available in only one patient [10]. A 26-year-old female with cerebellar γδ T-PCNSL underwent subtotal mass resection followed by high-dose methotrexate and cytarabine therapy, and she remained alive at 3 months [10]. Although our patient was lost to follow-up after surgery, lesions involving the spinal cord and brain had rapidly progressed before surgery.

The current revised 2016 World Health Organization (WHO) classification recognizes three entities of γδ T-cell lymphoma (γδ TCL) including hepatosplenic γδ TCL, primary cutaneous γδ TCL, and monomorphic epitheliotropic intestinal TCL [1]. However, γδ TCLs involving other extranodal sites were reported including the lung, orbit, and tongue [11-13]. Morphologic features of γδ TCL cells vary, but these cells often share the following immunophenotype: CD2(+), CD3(+), CD4(-), CD5(-), CD7(+/-), CD8(-/+), CD56(+/-), TIA1(+), granzyme B(+/-), perforin(+/-), TCRβF1(-), and TCRγ(+) [9]. In general, γδ TCL aggravates rapidly and responds poorly to standard chemotherapy [9]. Recently, recurrent genetic alterations involving the JAK/STAT pathway and epigenetic pathway were demonstrated in γδ TCLs [14,15]. Thus, it is necessary to gather clinical data of γδ TCLs and discover new therapeutics.

In summary, we report a unique case of γδ T-PCNSL involving the intramedullary spinal cord that presented with myelopathy. This case will intrigue and stimulate clinicians and pathologists to engage in the study of γδ TCL and T-PCNSL to discover effective therapeutic strategies and new targets for therapy.

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