Skip Navigation
Skip to contents

J Pathol Transl Med : Journal of Pathology and Translational Medicine

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
1 "Fixation procedures"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Article
Effects of Fixation and Storage of Human Tissue Samples on Nucleic Acid Preservation
Soo Kyung Nam, Joon Im, Yoonjin Kwak, Nayoung Han, Kyung Han Nam, An Na Seo, Hye Seung Lee
Korean J Pathol. 2014;48(1):36-42.   Published online February 25, 2014
DOI: https://doi.org/10.4132/KoreanJPathol.2014.48.1.36
  • 10,227 View
  • 167 Download
  • 37 Crossref
AbstractAbstract PDF
Background

Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.

Methods

To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed.

Results

All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks.

Conclusions

Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis.

Citations

Citations to this article as recorded by  
  • InDEL instability in two different tumoral tissues and its forensic significance
    İpek Gürel, Faruk Aşıcıoğlu, Gökhan Ersoy, Özlem Bülbül, Tülin Öztürk, Gönül Filoğlu
    Forensic Science, Medicine and Pathology.2024;[Epub]     CrossRef
  • Utility of bronchoscopically obtained frozen cytology pellets for next-generation sequencing
    Chihiro Mimura, Rei Takamiya, Shodai Fujimoto, Takafumi Fukui, Atsuhiko Yatani, Jun Yamada, Mizuki Takayasu, Naoya Takata, Hiroki Sato, Kiyoko Fukuda, Koichi Furukawa, Daisuke Hazama, Naoko Katsurada, Masatsugu Yamamoto, Shingo Matsumoto, Koichi Goto, Mot
    BMC Cancer.2024;[Epub]     CrossRef
  • Predictive molecular pathology after prolonged fixation: A study on tissue from anatomical body donors
    Anja Böckers, Leon Schurr, Michael Schön, Tatjana Scholl, Tobias M. Böckers, Konrad Steinestel, Annette Arndt
    Experimental and Molecular Pathology.2024; 137: 104899.     CrossRef
  • Tiempos y condiciones de almacenamiento de las muestras en anatomía patológica. Recomendaciones de la Sociedad Española de Anatomía Patológica parte 1: muestras destinadas al diagnóstico
    Francesc Tresserra Casas, Esther Rosello Sastre, María Jesús Fernández Aceñero, Lara Zaragoza Macián, Javier Azúa Romeo, Clara Alfaro-Cervelló, Samuel Navarro Fos, Eugenia García Fernández, Jordi Temprana-Salvador, Mar Iglesias Coma, Francesc Olivares Veg
    Revista Española de Patología.2024; 57(4): 235.     CrossRef
  • RNA-Seq Analysis in Non-Small Cell Lung Cancer: What Is the Best Sample from Clinical Practice?
    Lorenzo Nibid, Giovanna Sabarese, Luca Andreotti, Benedetta Canalis, Daniela Righi, Filippo Longo, Margherita Grazi, Pierfilippo Crucitti, Giuseppe Perrone
    Journal of Personalized Medicine.2024; 14(8): 851.     CrossRef
  • A Study on the Stainability and DNA Conservation of Tissue Slides according to Fixation Time and Temperature
    Da-som JEONG
    Korean Journal of Clinical Laboratory Science.2024; 56(3): 217.     CrossRef
  • Selection and Evaluation of mRNA and miRNA Reference Genes for Expression Studies (qPCR) in Archived Formalin-Fixed and Paraffin-Embedded (FFPE) Colon Samples of DSS-Induced Colitis Mouse Model
    Ana Unkovič, Emanuela Boštjančič, Aleš Belič, Martina Perše
    Biology.2023; 12(2): 190.     CrossRef
  • The quality of DNA isolated from autopsy formalin-fixed and formalin-fixed paraffin-embedded tissues: study of 1662 samples
    Katarina Vitošević, Danijela Todorović, Živana Slović, Tatjana Varljen, Ivana Radaković, Dušan Radojević, Vanja Čanović, Miloš Todorović
    Molecular Biology Reports.2023; 50(8): 6323.     CrossRef
  • Morphometric and Molecular Analysis of Five-Spine Epidinium Morphotypes Taken from the Rumen of European Bison, Bison bonasus
    Silvia Ivorová, Anna Kopčaková, Peter Pristaš, Svetlana Kišidayová
    Life.2023; 13(12): 2350.     CrossRef
  • Retrieving high-quality genomic DNA from formalin-fixed paraffin-embedded tissues for multiple molecular analyses
    Ha Thi Nguyen, Vinay Bharadwaj Tatipamula, Duy Ngoc Do, Thien Chi Huynh, Mai Kim Dang
    Preparative Biochemistry & Biotechnology.2022; 52(1): 48.     CrossRef
  • Evaluation of DNA Isolation and Amplification from Various Organs Preserved through Frozen, Formalin-Fixed and Paraffin-Embedded Tissue Sample method
    Mifta Rizqina Amalia, Anna Roosdiana, Yudit Oktanella, Andreas Bandang Hardian, Dini Agusti Paramanandi, Kharisma Kurnia Utami, Andi Tri Rakhmat Akbar, Made Venika Nareswari, Fajar Shodiq Permata
    Journal of Experimental Biology and Agricultural Sciences.2022; 10(3): 643.     CrossRef
  • DNA isolated from formalin-fixed paraffin-embedded healthy tissue after 30 years of storage can be used for forensic studies
    Katarina Vitošević, Miloš Todorović, Živana Slović, Tatjana Varljen, Stevan Matić, Danijela Todorović
    Forensic Science, Medicine and Pathology.2021; 17(1): 47.     CrossRef
  • Extraction of DNA and RNA from Formalin-fixed Paraffin-embedded Tissue Specimens
    NIdhi Shukla, Narmadhaa Siva, Madhu Sivakumar, Rabia Parveen, Ashwani Mishra, Avadh Shah, Krishna Medicherla, Prashanth Suravajhala
    BIO-PROTOCOL.2021;[Epub]     CrossRef
  • Cyclin dependent kinase inhibitor 2A and miR-671-5p expression profile in Iranian glioblastoma multiforme
    Tayyebali Salmani, Sayyed Mohammad Hossein Ghaderian, Mohammadreza Hajiesmaeili, Omidvar Rezaei Mirghaed, Azadeh Rakhshan, Mohammad Javad Nasiri, Mahan Mohammadi
    Gene Reports.2020; 19: 100620.     CrossRef
  • Comparison between Fluorescence in-situ Hybridization (FISH), Reverse Transcriptase PCR (RT-PCR) and fragment analysis, for detection of t (X; 18) (p11; q11) translocation in synovial sarcomas
    Omshree Shetty, Trupti Pai, Mamta Gurav, Bharat Rekhi
    Indian Journal of Pathology and Microbiology.2020; 63(1): 64.     CrossRef
  • Comparison of HPV detection rate in formalin‐fixed paraffin‐embedded tissues of head and neck carcinoma using two DNA extraction kits and three amplification methods
    Ljiljana Božić, Tanja Jovanović, Aleksandra Šmitran, Marko Janković, Aleksandra Knežević
    European Journal of Oral Sciences.2020; 128(6): 501.     CrossRef
  • Formalin Fixation of Human Healthy Autopsied Tissues: The Influence of Type of Tissue, Temperature and Incubation Time on the Quality of Isolated DNA
    Danijela Todorovic, Katarina Vitosevic, Milos Todorovic, Zivana Slovic
    Serbian Journal of Experimental and Clinical Research .2020; 21(4): 307.     CrossRef
  • Detection of Disease-specific Fusion Genes of Soft Tissue Tumors Using Formalin-fixed Paraffin-embedded Tissues; Its Diagnostic Usefulness and Factors Affecting the Detection Rates
    Takahiro Matsushige, Satoshi Kuwamoto, Michiko Matsushita, Lusi Oka Wardhani, Yasushi Horie, Kazuhiko Hayashi, Yukisato Kitamura
    Yonago Acta Medica.2019; 62(1): 115.     CrossRef
  • The Biospecimen Preanalytical Variables Program: A Multiassay Comparison of Effects of Delay to Fixation and Fixation Duration on Nucleic Acid Quality
    Latarsha J. Carithers, Rachana Agarwal, Ping Guan, Hana Odeh, Michael C. Sachs, Kelly B. Engel, Sarah R. Greytak, Mary Barcus, Conrado Soria, Chih-Jian (Jason) Lih, P. Mickey Williams, Philip A. Branton, Leslie Sobin, Benjamin Fombonne, Therese Bocklage,
    Archives of Pathology & Laboratory Medicine.2019; 143(9): 1106.     CrossRef
  • Pathobiology and innate immune responses of gallinaceous poultry to clade 2.3.4.4A H5Nx highly pathogenic avian influenza virus infection
    Kateri Bertran, Mary J. Pantin-Jackwood, Miria F. Criado, Dong-Hun Lee, Charles L. Balzli, Erica Spackman, David L. Suarez, David E. Swayne
    Veterinary Research.2019;[Epub]     CrossRef
  • Optimized Storage Methods of RNA Extraction from Formalin Fixed Paraffin Embedded Tissue
    Mehdi Barati, Mahdieh Shokrollahi Barough, Fatemeh Pak, Vahid Semnani, Mehrnoosh Pashaei, Parviz Kokhaei
    Middle East Journal of Rehabilitation and Health.2018;[Epub]     CrossRef
  • Effect of formalin fixation on pcr amplification of DNA isolated from healthy autopsy tissues
    Katarina Vitošević, Miloš Todorović, Tatjana Varljen, Živana Slović, Stevan Matić, Danijela Todorović
    Acta Histochemica.2018; 120(8): 780.     CrossRef
  • Evaluation of the optimal provision of formalin-fixed, paraffin-embedded material for reverse transcription-PCR in soft-tissue tumour diagnosis
    Khin Thway, Dorte Wren, Jasmin Lee, Lisa Thompson, Cyril Fisher, David Gonzalez
    Journal of Clinical Pathology.2017; 70(1): 20.     CrossRef
  • Molecular Testing for Gastrointestinal Cancer
    Hye Seung Lee, Woo Ho Kim, Yoonjin Kwak, Jiwon Koh, Jeong Mo Bae, Kyoung-Mee Kim, Mee Soo Chang, Hye Seung Han, Joon Mee Kim, Hwal Woong Kim, Hee Kyung Chang, Young Hee Choi, Ji Y. Park, Mi Jin Gu, Min Jin Lhee, Jung Yeon Kim, Hee Sung Kim, Mee-Yon Cho
    Journal of Pathology and Translational Medicine.2017; 51(2): 103.     CrossRef
  • Reference genes for studies in infectious parasitic diseases in five types of human tissues
    Cristina Silva Meira-Strejevitch, Vera Lucia Pereira-Chioccola, Marta Marques Maia, Daise Damaris Carnietto de Hippolito, Hui-Tzu Lin Wang, Gabriela Motoie, Aparecida Helena de Souza Gomes, Cristina Takami Kanamura, Roosecelis Brasil Martines, Cinara Cáss
    Gene Reports.2017; 7: 98.     CrossRef
  • Establishment of a primary culture of polymorphous low grade adenocarcinoma cells
    Lucas Novaes Teixeira, Victor Angelo Martins Montalli, Silvia Borges Pimentel de Oliveira, Thais Fernanda Santos Toledo, Elizabeth Ferreira Martinez, Vera Cavalcanti de Araújo
    Archives of Oral Biology.2017; 82: 188.     CrossRef
  • DNA degrades during storage in formalin-fixed and paraffin-embedded tissue blocks
    Alice Guyard, Alice Boyez, Anaïs Pujals, Cyrielle Robe, Jeanne Tran Van Nhieu, Yves Allory, Julien Moroch, Odette Georges, Jean-Christophe Fournet, Elie-Serge Zafrani, Karen Leroy
    Virchows Archiv.2017; 471(4): 491.     CrossRef
  • Prevalence of exon 11 internal tandem duplications in the C‐KIT proto‐oncogene in Australian canine mast cell tumours
    VS Tamlin, AE Kessell, RJ Mccoy, EC Dobson, TS Smith, M Hebart, L Brown, D Mitrovic, AE Peaston
    Australian Veterinary Journal.2017; 95(10): 386.     CrossRef
  • Robust transcriptional tumor signatures applicable to both formalin-fixed paraffin-embedded and fresh-frozen samples
    Rou Chen, Qingzhou Guan, Jun Cheng, Jun He, Huaping Liu, Hao Cai, Guini Hong, Jiahui Zhang, Na Li, Lu Ao, Zheng Guo
    Oncotarget.2017; 8(4): 6652.     CrossRef
  • Clinical impact of targeted amplicon sequencing for meningioma as a practical clinical-sequencing system
    Sayaka Yuzawa, Hiroshi Nishihara, Shigeru Yamaguchi, Hiromi Mohri, Lei Wang, Taichi Kimura, Masumi Tsuda, Mishie Tanino, Hiroyuki Kobayashi, Shunsuke Terasaka, Kiyohiro Houkin, Norihiro Sato, Shinya Tanaka
    Modern Pathology.2016; 29(7): 708.     CrossRef
  • Comparison of the Diagnostic Value Between Real-Time Reverse Transcription-Polymerase Chain Reaction Assay and Histopathologic Examination in Sentinel Lymph Nodes for Patients With Gastric Carcinoma
    Yoonjin Kwak, Soo Kyung Nam, Eun Shin, Sang-Hoon Ahn, Hee Eun Lee, Do Joong Park, Woo Ho Kim, Hyung-Ho Kim, Hye Seung Lee
    American Journal of Clinical Pathology.2016; 145(5): 651.     CrossRef
  • WITHDRAWN: Selection of reference genes in five types of human tissues for normalization of gene expression studies in infectious diseases
    Cristina Silva Meira-Strejevitch, Vera Lucia Pereira-Chioccola, Marta Marques Maia, Daise Damaris Carnietto de Hipólito, Hui-Tzu Lin Wang, Gabriela Motoie, Aparecida Helena de Souza Gomes, Cristina Takami Kanamura, Roosecelis Brasil Martines, Cinara Cássi
    Gene.2016;[Epub]     CrossRef
  • A Comparison of Fresh Frozen vs. Formalin-Fixed, Paraffin-Embedded Specimens of Canine Mammary Tumors via Branched-DNA Assay
    Florenza Lüder Ripoli, Annika Mohr, Susanne Conradine Hammer, Saskia Willenbrock, Marion Hewicker-Trautwein, Silvia Hennecke, Hugo Murua Escobar, Ingo Nolte
    International Journal of Molecular Sciences.2016; 17(5): 724.     CrossRef
  • Expression of caspase-3 predicts prognosis in advanced noncardia gastric cancer
    Sousana Amptoulach, Andreas C. Lazaris, Ioanna Giannopoulou, Nikolaos Kavantzas, Efstratios Patsouris, Nikolaos Tsavaris
    Medical Oncology.2015;[Epub]     CrossRef
  • The Importance of Reference Gene Analysis of Formalin-Fixed, Paraffin-Embedded Samples from Sarcoma Patients — An Often Underestimated Problem
    Ninna Aggerholm-Pedersen, Akmal Safwat, Steen Bærentzen, Marianne Nordsmark, Ole Steen Nielsen, Jan Alsner, Brita S. Sørensen
    Translational Oncology.2014; 7(6): 687.     CrossRef
  • Comparison of histomorphology and DNA preservation produced by fixatives in the veterinary diagnostic laboratory setting
    William F. Craft, Julia A. Conway, Michael J. Dark
    PeerJ.2014; 2: e377.     CrossRef
  • Histotechnical solutions for quality improvement of nucleic acid specimens extracted from paraffin blocks
    A. N Vaganova
    Genes & Cells.2014; 9(2): 96.     CrossRef

J Pathol Transl Med : Journal of Pathology and Translational Medicine
TOP