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- Diagnostic Value of
MDM2 andDDIT3 FluorescenceIn Situ Hybridization in Liposarcoma Classification: A Single-Institution Experience - Junhun Cho, Seung Eun Lee, Yoon-La Choi
- Korean J Pathol. 2012;46(2):115-122. Published online April 25, 2012
- DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.2.115
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Abstract
PDF Background The amplification of murine double minutes (
MDM2 ) is the primary feature of well-differentiated liposarcomas (WDLPS) and dedifferentiated liposarcomas (DDLPS), whileDDIT3 rearrangement is the main one of myxoid liposarcomas (MLPS). Our aim was to evaluate the added value ofMDM2 amplification andDDIT3 rearrangement in making a diagnosis and classifying lipogenic tumors.Methods Eighty-two cases of liposarcoma and 60 lipomas diagnosed between 1995 and 2010 were analysed for
MDM2 amplification andDDIT3 rearrangement using a fluorescencein situ hybridization (FISH). The subtypes of liposarcoma were reclassified according to the molecular results, whose results were reviewed with an analysis of the relevant histologic and immunohistochemical findings.Results One case of lipoma (1.67%) was reclassified as a WDLPS. Of the liposarcomas, 13.4% (16/82) were reclassified after the molecular testing. Five cases of MLPS were reclassified as four cases of DDLPS and one case of myxoid lipoma. Two cases of WDLPS were reclassified as one case of spindle cell lipoma and another case of myxofibrosarcoma. Four cases of DDLPS were reclassified as two cases of leiomyosarcoma, one case of angiomyolipoma and another case of fibroinflammatory lesion. Of the six cases of pleomorphic liposarcoma, five were reclassified as DDLPS.
Conclusions In our series, a critical revision of diagnosis was found at a rate of 3.5% (5/142) after a review of the lipomatous lesions. The uses of molecular testing by
MDM2 andDDIT3 FISH were valuable to make an accurate subtyping of liposarcomas as well as to differentiate WDLPS from benign lipomatous tumor.-
Citations
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Virchows Archiv.2024; 484(1): 71. CrossRef - FISH Diagnostic Assessment of MDM2 Amplification in Liposarcoma: Potential Pitfalls and Troubleshooting Recommendations
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