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Studies on Blastogenesis and T-Rostte Formation of Human Peripheral Blood Lymphocyte
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HOME > J Pathol Transl Med > Volume 11(2); 1977 > Article
Etc Studies on Blastogenesis and T-Rostte Formation of Human Peripheral Blood Lymphocyte
Journal of Pathology and Translational Medicine 1977;11(2):97-111
DOI: https://doi.org/
Department of General Surgery, and Clinical Pathology*, College of Medicine, Seoul National University, Seoul, Korea
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Various techniques to detect the immundozical functions of the lymphocytes has been developed. The authors establishing a stable techniques, performed the quantitations of rosette forming T-Iymphocyte(RP-TL) and phytohemagglutinin(PHA) stimulated Iymphocyte transformation rate(LTR) with 3H-thymidine uptake in in-vitro culture to define the characteristics of cellular immunity of Korean children, adults, and aged persons together with patients in various diseases. To standardize the potency of PHA, the authors used self-made saline extract of phaseolus vulgaris(PV) standardized by commercially available PHA. The results are summarized as follows : 1) The proportion of RF-TL was variable depending on individuality of sheeps ranging from 32±8.1 to 70.1±5.6% in total RF-TL and from 9.8±2.8 to 36.9±6.0% in active RF-TL in normal adnet. The most stable result was obtained by the concentration of 1.0% SRBC. 2) The proportion of total RF-TL in normal Koreans were 68.4±14.6% in adults, 65.6± 12.4% in children and 59.2±18.3% in old ages while the active RF-TL were 30.4±8.9% in children, 25.3±7, 4% in adults and 23.1±10.4% in old ages. 3) By means of our own self-made extract of P.V. the 3H-thymidine uptake of the peripheral blood lymphocytes was about 3% lower than that of foreign-made commercially available PHA but its blastogenesis was almost same with the latter. The PV-LTR were 68.6+17.6% in adult, 61.0+7.5% in old ages and 58.4±12.3% in children. 4) The RF-TL of cancer patients lower than that of normals and it was especially so in patients with extensive metastasis. The acute lymphoid leukemia patients disclosed the lowest RF-TL valves among the stuffy groups. 5) The PV-LTR in cancer patients was lowest in chronic lymphoid leukemia following the stomach cancer, lung cancer, hematoma, lymptoma and acute lymphoid leukemia in decreasing order of transformation rates. The PV-LTR were higher than normal controls in infectious mononucleosis and congenital syphilis while it was similar to normals in aplastic anemias. 6) The PV-LTR and RF-TL performed at the same time disclosed the following features ; a) In normal subjects, the RF-TL was lowest in old ages while the PV-LTR was lowest in children. b) In cancer patients, the PV-LTR was more significantly decreased than RF-TL suggesting more pronounced alteration or impairment of in vitro viability and/or DNA synthetic activity than the natural membrane-bound immune characters in their lymphocytes.

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