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The Korean Journal of Pathology 1978;12(1): 31-38.
Paper disk 법에 의한 탄수화물 분해 실험의 비교연구
A Study on the Possibility for the Replacement of the Conventional Carbohydrate Fermentation by the Disk paper Method
The use of carbohydrate fermentation tests has teen widely employed in the identification and the classification of various species of micro-organisms. The characteristics of the bacterial metabolism differ even within the same species depending upon its energy requirement a digestive patterns. The majority types of carbohydrate fermentation tests have been based on the fact that a certain micro-organism uses carbohydrates as an energy supply source and yields some metabolites by action of type specific enzymes. The study had been concentrated on the possibility whether the conventional fermentation tests could be replaced by the disk paper method, and concluded as following: 1. Between the data obtained by the test tube and the disk parer methods of carbohydrate fermentation, there was practically little discrepancy as for the fermentation pattern. 2. The most acceptable concentration of carbohydrates preparing the disk paper was 5 percent while 10 and 20 percents showed the zone of inhibition to the high acidity produced around the disks. 3. Generally, the acidification of the media can be read between 6 and 8 hours of incubation followed by the inoculation, therefore preliminary or complete identification can be cut at least one day. Consequently the laboratory data can reach at the physician’s hand a day earlier. 4. The disk paper method is proved to be much economic in the aspects of reagent, media, man power, and most of all, the time of identification still exhibiting the accuracy and the reliability.
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