One drop method of micro-TPHA(MHA-TP) using one drop of TP sensitized sheep red blood cells instead of three drops was evaluated for screening and the specific serological test for syphilis. Macro-tray method revealed almost same patterns of reaction in both two drops and 1 drop. In the later the ring of red cell pack is more dense and sharp than 2 drops method, in negative. The micro-tray method revealed also clear cut patterns of reaction in positive and negative results. The test cost may be reduced from 1/2 to 1/3 of original test cost by one drop method. The micro-tray method is prefered to recommend on account of the conveniency. One drop method appeared to be both the economical screening and specific confirmation for syphilis when one drop method is applied together with VDRL and RPR test.