Many studies have been reported by various observers on the bacteriology of acute and chronic cholecystitis. But only a few have reported in Korea. The purpose of the present investigation was to analyse the incidence of bacteria in acute and chronic cholecystitis and to define the predisposing factors of acalculous cholecystitis. Bacterial studies of the bile were made in 47 patients with acute and chronic cholecystitis who were operated upon on the Han Yang University Hospital during the 6 year period from 1975 to 1980. The following results were obtained. 1) In 95 cases of bile culture, total 77 strains of bacteria were isolated. The bacterial flora consisted of E. Coli 38 strains, Proteus 7 strains, Enterobacter 5 strains, Pseudomonas 4 strains, Klebsiella pneumoniae 4 strains, Enterococci 4 strains, Gram negactive nonfermentative bacilli 3 strains, Coliform bacilli 3 strains, Serratia 2 strains, and Providencia, Aerotacter, Alkaligenes faecalis, Staphylococcus epidermidis each one strain respectively. 2) In total 47 patients of cholecystitis, 76.6% were culture-pcstive. In 30 cases of chronic cholecystitis, 76.7% were culture-positive and in 17cases of acute cholecystitis, 76.5% were culture-positive, 3) Acalculous cholecystitis was 23.4% (11 cases), and hypochorhydria, cholangitis, liver abscess, pancreatic abscess, Ascaris lumbricoides, Clonerchis sinensis were regarded as predisposing factors.