A comparative evaluation of automated and manual platelet counting methods, and the influence on sample standing and continuous agitation on the platelet counts have been performed. The material used in this study consist of 60 cases of EDTA anticoagulated blood showing hematologically normal findings. The results was follows, 1) Generally, manual counting showed lower than automated counting values in normal ranges. 2) The differences of count between methodes using whole blood(Coulter Model S-Plus) and method using platelet rich plasma(Coulter Thrombocounter-C) were not significant, but the former was more time saving and reproducible. 3) The differences of count between standing and continuous agitation of blood samples for 20 hours in room temperature revealed no statistically significant changes, and they showed lower values than initial counts.