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The Korean Journal of Pathology 1983;17(1): 46-52.
흰쥐 췌장외 분비세포의 주사전자 현미경적 관찰
Scanning Electron Microscopy of the Ultrastructure of Pancreatic Acinar Cells
Several methods of tissue preparation and different modes of operation of the scanning electron microscope were used to study the ultrastructure of cells. The author examined ultrastructure of rat pancreatic acinar cells prepared by Osmium-DMSO-Osmium and subsequent cracked with razor blade, and discussed the scanning electron microscope images in comparison with light microscopic and transmission electron microscopic findings. The results were as follow. The ultrastructure of pancreatic acinar cells prepared by Osmium-DMSO-Osmium method was well exposed. The transmission electron microscopy easily revealed the cellular organelles which were poorly visualized by light microscope. The ultrastructures of pancreatic acinar cells studied by SEM were well correlated with results obtained by TEM. The surface of nuclear membrane was smooth. Mitochondria were round or elongated and had many cristae. RER arranged in lamellar pattern with occasional dilatation. Golgi complex was composed of vesicles, lamellae and vacuoles. The inner surfaces of above mentioned organelles revealed relatively granular textures. One important point revealed by cracked samples concerns the findings of spherical secretory granules within thin walled cavities, which suggestive of Golgi vesicle origin. These vesicles appear connected by small openings. It is suggested that they may represent a system of intercommunicating chambers(vacuoles).