Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Comparision of Biotin-Avidin System Method with Peroxidase-Antiperoxidase Method in Immunohistochemistry
Skip Navigation
Skip to contents

J Pathol Transl Med : Journal of Pathology and Translational Medicine

OPEN ACCESS
SEARCH
Search

Articles

Page Path
HOME > J Pathol Transl Med > Volume 17(4); 1983 > Article
Etc Comparision of Biotin-Avidin System Method with Peroxidase-Antiperoxidase Method in Immunohistochemistry
Journal of Pathology and Translational Medicine 1983;17(4):412-416
DOI: https://doi.org/
Department of Clinical Pathology, Chonnam University Medical School
  • 1,298 Views
  • 10 Download
  • 0 Crossref
  • 0 Scopus

Newly introduced BAS method in immunoperoxidase staining was compared with PAP method and the advantages and disadvantages of BAS method were discussed. Various dilutions of primary antisera of IgG, aFP, lysozyme, HSV II and GFA were applied to the surgical biopsy specimens of inflammatory reaction, hepatocellular carcinoma, gastric adenocarcinoma, cervical dysplasia and astrocytoma, respectively, and each specimen was stained with PAP or BAS method. The ratio of specific staining-background staining was higher in BAS method, where dilutions of the primary antisera were 4~16 times those applied in PAP method. The high dilution titers in BAS method was thought to be due to strong binding activity between biotin and avidin, and also due to lattice-like structure of ABC containing many peroxidase molecules. The main disadvantage of BAS method is endogenous avidin binding activity which can be overcome by pretreatment with avidin and biotin.

Related articles

J Pathol Transl Med : Journal of Pathology and Translational Medicine