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The Korean Journal of Pathology 2002;36(6): 400-405.
Expression of Major Gangliosides in Normal and Alzheimer Disease Brain.
Min Cheol Lee, Young Jong Woo, Seung U Kim, Tadashi Tai
1Department of Pathology and Pediatrics, Chonnam National University Medical School, Gwangju, Korea. mclee@chonnam.ac.kr
2Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju, Korea.
3Brain Disease Research Center, Ajou University, Suwon, Korea.
4Department of Tumor Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
ABSTRACT
BACKGROUND: GM1 ganglioside-bound amyloid beta-protein (GM1/A) has been reported to be involved with senile plaque formation in Alzheimer disease. METHODS: To investigate the binding of major gangliosides on senile plaques and neurofibrillary tangles of Alzheimer disease-specific pathology, we developed four monoclonal antibodies -- GM1, GD1a, GD1b, and GT1b -- employing the hydridoma technique, and applied them for immunohistochemical staining at the frontotemporal neocortex and hippocampus of Alzheimer disease brains and age-matched control brains. RESULTS: Moderate immunopositivity for GM1 and GD1a was noted on the senile plaques and neurofibrillary tangles. Mild immunopositivity for GD1b and GT1b on neurofibrillary tangles was noted. Strong GD1b immunopositivity was observed on a few neurons and neurites. Strong immunopositivity for GT1b, and moderate immunopositivity for GM1 and GD1a were noted on reactive astrocytes. CONCLUSIONS: These observations suggest that GM1 and GD1a may be involved in the formation of senile plaques as well as neurofibrillary tangles in Alzheimer disease brains.
Key Words: Alzheimer Disease; Gangliosides; Immunohistochemistry