CASE REPORT

A 36-year-old woman was admitted to our hospital for further evaluation and treatment of a mediastinal mass. The patient had no notable medical history. On chest radiography and computed tomography (CT) scans, the patient had a round, 5.6×4.4 cm-sized lobulated cystic mass in the anterior mediastium (Fig. 1A). To exclude a metastatic neoplasm to the mediastinum, we performed a comprehensive examination, including a detailed history taking; physical examinations including breast and pelvic examinations; upper and lower gastrointestinal endoscopic examinations; a CT examination of the chest, abdomen and pelvis; and a positron emission tomography and bone scans (Fig. 1B). But this disclosed no evidence of primary tumor elsewhere. The patient received a surgical excision of mediastinal mass, showing that the resected mediastinal mass consisted of a 9×6×5 cm-sized, round, tan-to-white, fairly well-circumscribed, lobulated mass (Fig. 1C). The cut surface revealed multiple, variable sized, multiloculated cysts separated by solid and semi-solid areas. The cysts had smooth walls and were filled with mucinous fluid (Fig. 1D). Microscopically, there was a residual presence of the normal thymic tissue with fatty replacement in the periphery of the lesion (Fig. 2A). The tumor showed solid and cystic areas that are lined by cuboidal to pseudostratified columnar epithelial cells. Some cystic walls underwent malignant transformation from the benign thymic epithelium (Fig. 2B). The solid area showed dilated glands, cords and small nests that infiltrated the fibrotic parenchyma (Fig. 2C). The neoplastic cells were abundant in mucinous cytoplasm with basally-located, mild-to-moderately pleomorphic, slightly hyperchromatic nuclei, which was accompanied by the presence of small nucleoli. In addition, there were also occasional mitoses with atypical forms. There were floating tumor cells in extracellular mucin pools (Fig. 2D). We performed an immunohistochemical staining using antibodies to cytokeratin (CK) 7, CK20, CD5, CDX-2 and thyroid transcription factor-1 (TTF-1) by serving appropriate negative and positive controls. This showed that the tumor cells were diffuse cytoplasmic and membranous positive for CK7, CK20 and focal nuclear positive for CDX-2 (Fig. 3A-C). Some tumor cells and normal thymic tissue were positive for CD5 (Fig. 3D). The primary pulmonary marker, TTF-1, was negative in both tumor cells and normal thymic tissue.

Postoperatively, the patient was followed up after receiving radical-aimed radiotherapy and chemotherapy (six cycles of Taxol/CDDP regimen). On chest CT scans at a 1-year follow-up, the patient had multiple metastatic nodules in both lungs with malignant pleural effusion. On postoperative month 15, the patient died of disease.

DISCUSSION

Primary thymic carcinoma is a rare entity that poses a diagnostic challenge for both clinicians and pathologists because of their infrequent occurrence and variable histological features.2 The majority of reported cases of primary thymic carcinomas are squamous cell carcinoma or its variants.6 Of the primary adenocarcinomas, mucinous adenocarcinoma is the rarest histologic subtype. Only nine cases have been documented since it was first reported by Choi et al.4 in 2003.2,3,5-7

In making a proper diagnosis of primary thymic mucinous adenocarcinoma, the clinicopathologic and radiological findings are needed. Clinically, the tumor should be confined to the anterior mediastinum with no evidence of tumor in other organs.3 Histopathologically, the malignant transformation of benign epithelial cells of the thymic cyst is essential for making a correct diagnosis of the primary thymic adenocarcinoma. These lining epithelial cells, whether they are benign or malignant, are positive for CD5, a marker of thymic epithelial cells. In our case, there was a transitional zone where the CD5-positive, benign epithelial cells lining the cyst underwent malignant transformation.

Primary mucinous adenocarcinoma of the thymus is a diagnosis of exclusion. On routine microscopic examination alone, the tumor is suggestive of mucinous adenocarcinoma occurring in the gastrointestinal tract, pancreaticobiliary tract, lung, breast and ovary.7 In addition, adenocarcinoma arising in a mediastinal teratoma, thymic carcinoid tumor with prominent mucinous stroma or mucoepidermoid carcinoma should be considered in the differential diagnosis.6 The carcinoid tumor with prominent mucinous stroma is composed of a conventional neuroendocrine morphology such as an organoid pattern and a delicate fibrovascular septum. In establishing a diagnosis as mucoepidermoid carcinoma, the histological findings should include both the squamous and the mucin-producing components. In addition, all the three germ cell layers should also be present for making a diagnosis of mediastinal teratoma. But these findings were not found in our case.

Presumably, the respiratory tract might be one of the most common primary sites of the occurrence of metastatic mediastinal adenocarcinoma.2 It is known that TTF-1 is a very specific marker for the primary pulmonary adenocarcinoma.6 It is not expressed in cases of thymic mucinous adenocarcinoma. But it is expressed in 77-100% of total cases of pulmonary mucinous adenocarcinoma.2 We think that TTF-1 is a very useful marker for differentiating thymic and primary pulmonary mucinous adenocarcinomas.

There are no specific immunohistochemical markers for the tumors of thymic origin. CD5, a lymphocytic marker, is expressed in approximately 70% of all cases of thymic adenocarcinoma and it is relatively uncommon in non-thymic carcinomas.6

Immunostaining for CK7, CK20, and CDX-2 is helpful for the differential diagnosis of mucnious adenocarcinoma from other thymic carcinomas, such as adenocarcinoma with mucinous differentiation arising in a mediastinal teratoma, thymic carcinoid tumor with prominent mucinous stroma or mucoepidermoid carcinoma with mucinous differentiation. However, these immunostains are not helpful for differentiation of primary thymic mucinous adenocarcinomas from metastatic mucinous one. This is due to the positive staining of those markers in pulmonary, gastrointestinal and ovarian mucinous adenocarcinomas. In our case, immunohistochemical stains of tumor cells were diffuse positive for CK7 and CK20; focal positive for CD5 and CDX-2; and negative for TTF-1. These findings are close to those seen in a case reported by Choi et al.4 In our case, the cysts met all the diagnostic criteria for an acquired multilocular thymic cyst.7 It has been postulated that the thymic epithelium would potentially undergo glandular differentiation, as evidenced by the presence of ciliated and mucinous cells in the epithelium of the cysts.4 A rare instance of malignant transformation of thymic cyst had also been described,2-4,7 and it was focally present in our case. It is difficult to describe the prognosis of this rare entity because of a smaller number of cases. It appears, however, that the thymic mucinous adenocarcinoma may behave aggressively than the non-mucinous one.7 With a review of literatures and based on our case, it can be concluded that thymic mucinous adenocarcinoma occurs in relatively young age with an average age of 47 years (range, 15 to 82 years) and with a male-to-female ratio of 1:1. The tumor size is large with an average size of 10.27 cm (range, 5.2 to 14.5 cm) and 50% (5/10) of cases are associated with cystic change. All of the patients with mucinous thymic adenocarcinoma died of disease or had metastatic lesions few months after the diagnosis (Table 1).

In conclusion, we believe that this aggressive subtype of thymic adenocarcinoma should be regarded as a distinct variant of thymic carcinoma. It is an extremely rare tumor for which a comprehensive, clinical, radiological and pathological work-up should be done to make a correct diagnosis of and to promptly manage it.

REFERENCES

• 1. Suster S. Thymic carcinoma: update of current diagnostic criteria and histologic types. Semin Diagn Pathol 2005; 22: 198-212. ArticlePubMed
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Fig. 1

Radiologic and gross findings. (A) The chest computed tomography (CT) shows a 5.6×4.4 cm sized, multiloculated cystic mass (arrow) with heterogenous enhancing septa (37→57 hounsfield unit) in the right anterior mediastinum. (B) On positron emission tomography-CT scans, the solid part of the mass shows nodular F-18 fluorodeoxyglucose (FDG) uptake. There is no remarkable FDG uptake in the entire body. (C) A round mass with a smooth, lobulated external surface. (D) The cut surface reveals yellow-white solid mass with multiple cysts of variable sizes, filled with mucinous fluid.

Fig. 2

Microscopic findings. (A) The low microscopic field shows the residual thymic tissue in lymphocyte-rich cortex (right lower) and multifocal cyst formation. (B) The cyst is lined by malignant mucious epithelium undergoing the transition from the benign flattened thymic epithelium of the cyst wall. (C) The malignant mucinous glands infiltrates in the fibrotic parenchyma of tumor. (D) Island of atypical cells floats in pools of extracellular mucin.

Fig. 3

Immunohistochemical stains. (A) The immunhistochemical stains reveal strong positivity for cytokeratin (CK) 7 and (B) CK20 and focal positivity for (C) CDX-2. (D) The thymic tissue and epithelium lining the thymic (benign) cyst shows a positive reaction for CD5.

Table 1

Summary of the reported cases of thymic mucinous adenocarcinoma

F, female; S, resection; RTx, radiotherapy; CTx, chemotherapy; CT, computed tomography; DOD, died of disease; N/A, no information available; MTS, metastasis; AWD, alive with disease; M, male; Diss, dissemination; NED, no evidence of disease.

Figure & Data

References

Citations

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