Journal of Pathology and Translational Medicine

Case Study

Hepatocellular Carcinoma Arising in a Huge Hepatocellular Adenoma with Bone Marrow Metaplasia: Hyo Jeong Kang, Hui Jeong Jeong, So-Woon Kim, Eunsil Yu, Young-Joo Lee, So Yeon Kim, Jihun Kim; J Pathol Transl Med. 2018;52(4):226-231. Published online December 27, 2017; DOI: https://doi.org/10.4132/jptm.2017.11.12

6,387 View
149 Download
5 Web of Science
6 Crossref

Hepatocellular adenoma (HCA) is the most common type of benign liver tumor, and its major complication is malignant transformation to hepatocellular carcinoma (HCC). Here, we report a case of HCC arising in HCA with bone marrow metaplasia in a 24-year-old Korean woman who presented with abdominal discomfort. A huge liver mass was found on abdominal ultrasonography. She underwent surgical hepatic resection, and the resected specimen was entirely involved by a 20-cm-sized tumor. Histological review revealed a well differentiated HCC arising from inflammatory HCA with β-catenin nuclear positivity and bone marrow metaplasia that contained hematopoietic cells. This case was unique because malignant transformation, inflammatory type HCA, β-catenin nuclear staining, and bone marrow metaplasia were simultaneously observed. Additionally, it should be noted that a large HCA with β-catenin activation can undergo malignant transformation and should be surgically resected in a timely manner.

Citations

Citations to this article as recorded by

Adult Hepatocellular Carcinoma Coexisting with Extramedullary Hematopoiesis
Hirotsugu Noguchi, Michiyo Higashi, Ryo Desaki, Takashi Tasaki, Mari Kirishima, Ikumi Kitazono, Kazuhiro Tabata, Akihide Tanimoto
International Journal of Surgical Pathology.2022; 30(3): 339. CrossRef
Spontaneous Occurrence of Various Types of Hepatocellular Adenoma in the Livers of Metabolic Syndrome-Associated Steatohepatitis Model TSOD Mice
Wenhua Shao, Orgil Jargalsaikhan, Mayuko Ichimura-Shimizu, Qinyi Cai, Hirohisa Ogawa, Yuko Miyakami, Kengo Atsumi, Mitsuru Tomita, Mitsuko Sutoh, Shunji Toyohara, Ryoji Hokao, Yasusei Kudo, Takeshi Oya, Koichi Tsuneyama
International Journal of Molecular Sciences.2022; 23(19): 11923. CrossRef
Bilateral Diffuse Nodular Pulmonary Ossification Mimicking Metastatic Disease in a Patient with Fibrolamellar Hepatocellular Carcinoma
Pattamon Sutthatarn, Cara E. Morin, Jessica Gartrell, Wayne L. Furman, Max R. Langham, Teresa Santiago, Andrew J. Murphy
Children.2021; 8(3): 226. CrossRef
Malignant transformation of liver fatty acid binding protein-deficient hepatocellular adenomas: histopathologic spectrum of a rare phenomenon
Juan Putra, Linda D. Ferrell, Annette S.H. Gouw, Valerie Paradis, Arvind Rishi, Christine Sempoux, Charles Balabaud, Swan N. Thung, Paulette Bioulac-Sage
Modern Pathology.2020; 33(4): 665. CrossRef
Hepatocellular carcinoma arising from hepatic adenoma in a young woman
Haythem Yacoub, Hela Kchir, Dhouha Cherif, Hajer Hassine, Slim Haouet, Asma Ayari, Habiba Mizouni, Saber Mannai, Mohamed Tahar Khalfallah, Nadia Maamouri
Clinical Case Reports.2020; 8(9): 1659. CrossRef
Metanephric adenoma with osseous metaplasia and bone marrow elements
Alessandro Pietro Aldera, Jeff John, Dharshnee Chetty, Dhirendra Govender
Human Pathology: Case Reports.2019; 17: 200316. CrossRef

Original Articles

Proposal of an Appropriate Decalcification Method of Bone Marrow Biopsy Specimens in the Era of Expanding Genetic Molecular Study: Sung-Eun Choi, Soon Won Hong, Sun Och Yoon; J Pathol Transl Med. 2015;49(3):236-242. Published online May 15, 2015; DOI: https://doi.org/10.4132/jptm.2015.03.16

13,617 View
291 Download
45 Web of Science
47 Crossref

Abstract PDF

Background
The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material is necessary. Methods: Pairs of bilateral bone marrow biopsies sampled from 53 patients were decalcified according to protocols of two comparison groups: EDTA versus HCl and RDO GOLD (RDO) versus HCl. Pairs of right and left bone marrow biopsy samples harvested from 28 cases were allocated into the EDTA versus HCl comparison group, and 25 cases to the RDO versus HCl comparison group. The decalcification protocols were compared with regards to histomorphology, immunohistochemistry, and molecular analysis. For molecular analysis, we randomly selected 5 cases from the EDTA versus HCl and RDO versus HCl groups. Results: The decalcification time for appropriate histomorphologic analysis was the longest in the EDTA method and the shortest in the RDO method. EDTA was superior to RDO or HCl in DNA yield and integrity, assessed via DNA extraction, polymerase chain reaction, and silver in situ hybridization using DNA probes. The EDTA method maintained intact nuclear protein staining on immunohistochemistry, while the HCl method produced poor quality images. Staining after the RDO method had equivocal results. RNA in situ hybridization using kappa and lambda RNA probes measured RNA integrity; the EDTA and RDO method had the best quality, followed by HCl. Conclusions: The EDTA protocol would be the best in preserving genetic material. RDO may be an acceptable alternative when rapid decalcification is necessary.

Citations

Citations to this article as recorded by

Optimization of Formic Acid-Formalin-Based Decalcification Protocol for Rat Calvarial Bone Histology
S. Amitha Banu, Khan Sharun, Merlin Mamachan, Athira Subash, Vadapalli Deekshita, Kirtika Sharma, Karikalan Mathesh, Obli Rajendran Vinodh kumar, Swapan Kumar Maiti, Abhijit M. Pawde, Laith Abualigah, Kuldeep Dhama, Amarpal
Journal of Experimental Biology and Agricultural Sciences.2024; 12(2): 218. CrossRef
Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow
Gabriella M. L. Diamantino, Janet Beeler-Marfisi, Robert A. Foster, William Sears, Alice Defarges, William Vernau, Dorothee Bienzle
Veterinary Pathology.2024; 61(6): 943. CrossRef
In situ metabolomic analysis of osteonecrosis of the femoral head (ONFH) using MALDI MSI
Chen Li, Jikun Liu, Yiqi Sheng, Yinghao Wang, Lan Jia, Yinguang Zhang, Jiantao Li, Shuangshuang Di, Honggang Nie, Yehua Han
Analytical and Bioanalytical Chemistry.2024; 416(23): 5155. CrossRef
A set of pretreatment reagents including improved formula fixation and decalcification facilitating immunohistochemistry and DNA analyses of formalin-fixed paraffin-embedded bone marrow trephine biopsy
Ting Sun, Liming Xu, Hongtian Yao, Jing Zhao, Zhen Chen, Zexin Chen, Bo Wang, Wei Ding
Acta Histochemica.2024; 126(8): 152188. CrossRef
Germline and somatic testing for homologous repair deficiency in patients with prostate cancer (part 1 of 2)
Andrew J. Armstrong, Amy Taylor, Michael C. Haffner, Wassim Abida, Alan H. Bryce, Lawrence I. Karsh, Scott T. Tagawa, Przemyslaw Twardowski, Anthony V. Serritella, Joshua M. Lang
Prostate Cancer and Prostatic Diseases.2024;[Epub] CrossRef
To Freeze or Not to Freeze? Recommendations for Intraoperative Examination and Gross Prosection of Thyroid Glands
Fouad R. Zakka, Nicole A. Cipriani
Surgical Pathology Clinics.2023; 16(1): 15. CrossRef
Effect of Surface Decalcification With Hydrochloric Acid on the Determination of Estrogen Receptor, Progesterone Receptor, Ki67, and Human Epidermal Growth Factor Receptor 2 Expressions in Invasive Breast Carcinoma Based on Immunohistochemistry and Fluore
Wu Ping, Rao Xin, Zhang Li, Chen Yupeng, Song Fangling, Ren Caihong, Hu Shun, Zhang Sheng
Applied Immunohistochemistry & Molecular Morphology.2023; 31(4): 232. CrossRef
Diagnostic value of MDM2 RNA in situ hybridization for low-grade osteosarcoma: Consistency comparison of RNA in situ hybridization, fluorescence in situ hybridization, and immunohistochemistry
Chen Chen, Xin He, Min Chen, Tianhai Du, Weiji Qin, Wenyi Jing, Hongying Zhang
Virchows Archiv.2023; 482(6): 999. CrossRef
Bone marrow fibrosis is associated with non‐response to CD19 CAR T‐cell therapy in B‐acute lymphoblastic leukemia
Joshua Anil, Ahab Alnemri, Andrew Lytle, Brian Lockhart, Ashley E. Anil, Michael Baumgartner, Kirubel Gebre, Jared McFerran, Stephan A. Grupp, Susan R. Rheingold, Vinodh Pillai
American Journal of Hematology.2023; 98(12): 1888. CrossRef
Epithelioid haemangioendothelioma of the mandible – A case report and review of the literature
Ali Rizvi, Tim K. Blackburn, Guy N. J. Betts
Oral Surgery.2022; 15(3): 387. CrossRef
Evaluation of EDTA and nitric acid solutions for decalcification of joints in AG/WT, BALB/c, C57, DBA1/J mice, and in Wistar rats
Eduarda Correa Freitas, Suelen Pizzolatto Dalmolin, Mateus Müller da Silva, Francine Hehn de Oliveira, Emily Ferreira Salles Pilar
Biotechnic & Histochemistry.2022; 97(5): 372. CrossRef
Coupling Lipid Labeling and Click Chemistry Enables Isolation of Extracellular Vesicles for Noninvasive Detection of Oncogenic Gene Alterations
Na Sun, Benjamin V. Tran, Zishan Peng, Jing Wang, Ceng Zhang, Peng Yang, Tiffany X. Zhang, Josephine Widjaja, Ryan Y. Zhang, Wenxi Xia, Alexandra Keir, Jia‐Wei She, Hsiao‐hua Yu, Jing‐Jong Shyue, Hongguang Zhu, Vatche G. Agopian, Renjun Pei, James S. Toml
Advanced Science.2022;[Epub] CrossRef
The Expressions of CD30 and CD123 of Mastocytosis in Taiwan
Ching-Fen Yang, Chih-Yi Hsu
Applied Immunohistochemistry & Molecular Morphology.2022; 30(4): 278. CrossRef
Unusual Patterns of HER2 Expression in Breast Cancer: Insights and Perspectives
Dora Grassini, Eliano Cascardi, Ivana Sarotto, Laura Annaratone, Anna Sapino, Enrico Berrino, Caterina Marchiò
Pathobiology.2022; 89(5): 278. CrossRef
Expert opinion on NSCLC small specimen biomarker testing — Part 1: Tissue collection and management
Frédérique Penault-Llorca, Keith M. Kerr, Pilar Garrido, Erik Thunnissen, Elisabeth Dequeker, Nicola Normanno, Simon J. Patton, Jenni Fairley, Joshua Kapp, Daniëlle de Ridder, Aleš Ryška, Holger Moch
Virchows Archiv.2022; 481(3): 335. CrossRef
Comparison of bone demineralisation procedures for DNA recovery from burned remains
Meghan Mckinnon, Denice Higgins
Forensic Science International: Genetics.2021; 51: 102448. CrossRef
A review of the current understanding of burned bone as a source of DNA for human identification
Meghan Mckinnon, Maciej Henneberg, Denice Higgins
Science & Justice.2021; 61(4): 332. CrossRef
Time is bone — Quantitative comparison of decalcification solvents in human femur samples using dual-X-ray-absorptiometry and computed tomography
Joshua Gawlitza, Jakob Steinhäuser, Arno Bücker, Gabriela Krasteva-Christ, Thomas Tschernig
Annals of Anatomy - Anatomischer Anzeiger.2021; 235: 151696. CrossRef
Molecular biomarker testing for non–small cell lung cancer: consensus statement of the Korean Cardiopulmonary Pathology Study Group
Sunhee Chang, Hyo Sup Shim, Tae Jung Kim, Yoon-La Choi, Wan Seop Kim, Dong Hoon Shin, Lucia Kim, Heae Surng Park, Geon Kook Lee, Chang Hun Lee
Journal of Pathology and Translational Medicine.2021; 55(3): 181. CrossRef
Effect of EDTA decalcification on estrogen receptor and progesterone receptor immunohistochemistry and HER2/neu fluorescence in situ hybridization in breast carcinoma
Erik Washburn, Xiaoyu Tang, Carla Caruso, Michelle Walls, Bing Han
Human Pathology.2021; 117: 108. CrossRef
Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis
Emna Romdhane, Soumaya Rammeh, Chelli Mouna Bouaziz, Hend Riahi, Meriam Rekaya Ben, Meriam Ksentini, Yosra Chebbi, Wafa Achour, Asma Ferjani, Ben Boubaker Ilhem Boutiba, Leila Slim-Saidi, Mohamed Fethi Ladeb
Clinical Rheumatology.2021; 40(10): 4317. CrossRef
Molecular Characterization of Prostate Cancers in the Precision Medicine Era
Emilio Francesco Giunta, Laura Annaratone, Enrico Bollito, Francesco Porpiglia, Matteo Cereda, Giuseppe Luigi Banna, Alessandra Mosca, Caterina Marchiò, Pasquale Rescigno
Cancers.2021; 13(19): 4771. CrossRef
Increased NF-κB Activity in Osteoprogenitor-Lineage Cells Impairs the Balance of Bone Versus Fat in the Marrow of Skeletally Mature Mice
Tzuhua Lin, Jukka Pajarinen, Yusuke Kohno, Akira Nabeshima, Laura Lu, Karthik Nathan, Zhenyu Yao, Joy Y. Wu, Stuart Goodman
Regenerative Engineering and Translational Medicine.2020; 6(1): 69. CrossRef
Percutaneous CT-guided biopsy of lytic bone lesions in patients clinically suspected of lung cancer: Diagnostic performances for pathological diagnosis and molecular testing
Anne-Claire Toffart, Stéphane Asfari, Anne Mc Leer, Emilie Reymond, Adrien Jankowski, Denis Moro-Sibilot, Olivier Stephanov, Julien Ghelfi, Sylvie Lantuejoul, Gilbert R. Ferretti
Lung Cancer.2020; 140: 93. CrossRef
Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples
Elodie Miquelestorena-Standley, Marie-Lise Jourdan, Christine Collin, Corinne Bouvier, Frédérique Larousserie, Sébastien Aubert, Anne Gomez-Brouchet, Jean-Marc Guinebretière, Matthias Tallegas, Bénédicte Brulin, Louis-Romée Le Nail, Anne Tallet, François
Modern Pathology.2020; 33(8): 1505. CrossRef
Identifying Opportunities and Challenges for Patients With Sarcoma as a Result of Comprehensive Genomic Profiling of Sarcoma Specimens
Margaret A. Hay, Eric A. Severson, Vincent A. Miller, David A. Liebner, Jo-Anne Vergilio, Sherri Z. Millis, James L. Chen
JCO Precision Oncology.2020; (4): 176. CrossRef
Comparison of Methods for the Histological Evaluation of Odontocete Spiral Ganglion Cells
Tania Ramírez, Simona Sacchini, Yania Paz, Rubén S. Rosales, Nakita Câmara, Marisa Andrada, Manuel Arbelo, Antonio Fernández
Animals.2020; 10(4): 683. CrossRef
Molecular Pathology of Primary Non-small Cell Lung Cancer
David Ilan Suster, Mari Mino-Kenudson
Archives of Medical Research.2020; 51(8): 784. CrossRef
Comparison of ethylenediaminetetraacetic acid and rapid decalcificier solution for studying human temporal bones by immunofluorescence
Sumana Ghosh, Mark B. Lewis, Bradley J. Walters
Laryngoscope Investigative Otolaryngology.2020; 5(5): 919. CrossRef
A novel cryo-embedding method for in-depth analysis of craniofacial mini pig bone specimens
Pavla Ticha, Igor Pilawski, Xue Yuan, Jie Pan, Ustun S. Tulu, Benjamin R. Coyac, Waldemar Hoffmann, Jill A. Helms
Scientific Reports.2020;[Epub] CrossRef
Accelerating precision medicine in metastatic prostate cancer
Joaquin Mateo, Rana McKay, Wassim Abida, Rahul Aggarwal, Joshi Alumkal, Ajjai Alva, Felix Feng, Xin Gao, Julie Graff, Maha Hussain, Fatima Karzai, Bruce Montgomery, William Oh, Vaibhav Patel, Dana Rathkopf, Matthew Rettig, Nikolaus Schultz, Matthew Smith,
Nature Cancer.2020; 1(11): 1041. CrossRef
Tissue Morphology and Antigenicity in Mouse and Rat Tibia: Comparing 12 Different Decalcification Conditions
Kristofor Bogoevski, Anna Woloszyk, Keith Blackwood, Maria A. Woodruff, Vaida Glatt
Journal of Histochemistry & Cytochemistry.2019; 67(8): 545. CrossRef
Cellular and collagen reference values of gingival and periodontal ligament tissues in rats: a pilot study
Antoine Alves, Nina Attik, Carine Wirth, Yves Bayon, Alexis Piat, Brigitte Grosgogeat, Kerstin Gritsch
Histochemistry and Cell Biology.2019; 152(2): 145. CrossRef
Implementing Precision Medicine Programs and Clinical Trials in the Community-Based Oncology Practice: Barriers and Best Practices
Jennifer L. Ersek, Lora J. Black, Michael A. Thompson, Edward S. Kim
American Society of Clinical Oncology Educational Book.2018; (38): 188. CrossRef
Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL
Zandra C Deans, Jose Luis Costa, Ian Cree, Els Dequeker, Anders Edsjö, Shirley Henderson, Michael Hummel, Marjolijn JL Ligtenberg, Marco Loddo, Jose Carlos Machado, Antonio Marchetti, Katherine Marquis, Joanne Mason, Nicola Normanno, Etienne Rouleau, Ed S
Virchows Archiv.2017; 470(1): 5. CrossRef
Protocolo para el estudio de muestras y estandarización del informe patológico de tumores óseos
Isidro Machado, José Juan Pozo, David Marcilla, Julia Cruz, Juan C. Tardío, Aurora Astudillo, Sílvia Bagué
Revista Española de Patología.2017; 50(1): 34. CrossRef
Extremely Well-Differentiated Papillary Thyroid Carcinoma Resembling Adenomatous Hyperplasia Can Metastasize to the Skull: A Case Report
Ju Yeon Pyo, Jisup Kim, Sung-eun Choi, Eunah Shin, Seok-Woo Yang, Cheong Soo Park, Seok-Mo Kim, SoonWon Hong
Yonsei Medical Journal.2017; 58(1): 255. CrossRef
Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species
Guoying Deng, Kerun Niu, Feng Zhou, Buxiao Li, Yingjie Kang, Xijian Liu, Junqing Hu, Bo Li, Qiugen Wang, Chengqing Yi, Qian Wang
Scientific Reports.2017;[Epub] CrossRef
Precision Medicine Starts With Preanalytics: Real-Time Assessment of Tissue Fixation Quality by Ultrasound Time-of-Flight Analysis
Melissa L. Lerch, Daniel R. Bauer, David Chafin, Abbey Theiss, Michael Otter, Geoffrey S. Baird
Applied Immunohistochemistry & Molecular Morphology.2017; 25(3): 160. CrossRef
Good Laboratory Standards for Clinical Next-Generation Sequencing Cancer Panel Tests
Jihun Kim, Woong-Yang Park, Nayoung K. D. Kim, Se Jin Jang, Sung-Min Chun, Chang-Ohk Sung, Jene Choi, Young-Hyeh Ko, Yoon-La Choi, Hyo Sup Shim, Jae-Kyung Won
Journal of Pathology and Translational Medicine.2017; 51(3): 191. CrossRef
An international survey about nail histology processing techniques
Christina Wlodek, Pauline Lecerf, Josette Andre, Beth S. Ruben, David de Berker
Journal of Cutaneous Pathology.2017; 44(9): 749. CrossRef
pSTAT5 and ERK exhibit different expression in myeloproliferative neoplasms
Ewa Wiśniewska-Chudy, Łukasz Szylberg, Grzegorz Dworacki, Ewa Mizera-Nyczak, Andrzej Marszałek
Oncology Reports.2017; 37(4): 2295. CrossRef
How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days
I. de Laak–de Vries, A. G. Siebers, L. Burgers, C. Diepenbroek, M. Link, P. Groenen, J. H. J. M. van Krieken, K. M. Hebeda
Journal of Hematopathology.2016; 9(2): 67. CrossRef
Do More With Less: Tips and Techniques for Maximizing Small Biopsy and Cytology Specimens for Molecular and Ancillary Testing: The University of Colorado Experience
Dara L. Aisner, Mathew D. Rumery, Daniel T. Merrick, Kimi L. Kondo, Hala Nijmeh, Derek J. Linderman, Robert C. Doebele, Natalie Thomas, Patrick C. Chesnut, Marileila Varella-Garcia, Wilbur A. Franklin, D. Ross Camidge
Archives of Pathology & Laboratory Medicine.2016; 140(11): 1206. CrossRef
Analysis of the Effects of Bone Marrow Biopsy Decalcification Methods on Histopathological Examination
Ji Young Park, Kyung Hee Han
The Korean Journal of Clinical Laboratory Science.2016; 48(4): 371. CrossRef
Distinguishing between Microbial Habitats Unravels Ecological Complexity in Coral Microbiomes
Amy Apprill, Laura G. Weber, Alyson E. Santoro, Nicole S. Webster
mSystems.2016;[Epub] CrossRef
Optimal Fixation and Decalcification Methods for Bone Marrow Biopsy
Myung-Sub Choi, Hyunsup Lee, Hyuk-Chul Kwon, Moon-Hwan Bae, Young-Hye Ko, Hee-Jin Kim, Beom-Se Lee, Bon-Kyung Koo
Korean Journal of Clinical Laboratory Science.2015; 47(4): 243. CrossRef

Morphologic Alteration of Metastatic Neuroblastic Tumor in Bone Marrow after Chemotherapy: Go Eun Bae, Yeon-Lim Suh, Ki Woong Sung, Jung-Sun Kim; Korean J Pathol. 2013;47(5):433-442. Published online October 25, 2013; DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.5.433

6,634 View
42 Download
1 Crossref

Abstract PDF

Background

The aim of this study is to evaluate the histologic features of metastatic neuroblastic tumors (NTs) in bone marrow (BM) before and after chemotherapy in comparison with those of primary NTs.

Methods

A total of 294 biopsies from 48 children diagnosed with NTs with BM metastasis were examined. There were 48 primary neoplasm biopsies, 48 BM biopsies before chemotherapy, 36 primary neoplasm excisional biopsies after chemotherapy, and 162 BM biopsies after chemotherapy.

Results

Metastatic NTs in BM before chemotherapy were composed of undifferentiated and/or differentiating neuroblasts, but had neither ganglion cells nor Schwannian stroma. Metastatic foci of BM after chemotherapy were found to have differentiated into ganglion cells or Schwannian stroma, which became more prominent after further cycles of chemotherapy. Persistence of NTs or tumor cell types in BM after treatment did not show statistically significant correlation to patients' outcome. However, three out of five patients who newly developed poorly differentiated neuroblasts in BM after treatment expired due to disease progression.

Conclusions

Metastatic NTs in BM initially consist of undifferentiated or differentiating neuroblasts regardless of the primary tumor subtype, and become differentiated after chemotherapy. Newly appearing poorly differentiated neuroblasts after treatment might be an indicator for poor prognosis.

Citations

Citations to this article as recorded by

Postchemotherapy gross residual tumor in non‐high‐risk neuroblastoma: Clinical significance and the role of adjuvant therapy
Eun Seop Seo, Hana Lim, Hee Won Cho, Hee Young Ju, Ji Won Lee, Keon Hee Yoo, Sanghoon Lee, Do Hoon Lim, Ki Woong Sung, Hong Hoe Koo
Pediatric Blood & Cancer.2022;[Epub] CrossRef

Case Report

Cytologic Findings of Polyomavirus Infection in the Urine: A Case Report.: Mi Seon Kwon, Young Shin Kim, Kyo Young Lee, Yeong Jin Choi, Chang Suk Kang, Sang In Shim; Korean J Cytopathol. 1996;7(2):192-196.

1,897 View
24 Download

Abstract PDF: The principal significance of the urothelial changes caused by polyomavirus activation is in an erroneous diagnosis of urothelial cancer; however, the clue to their benign nature is the smooth structureless nuclear configuration and the relative paucity of affected cells. Though virologic studies and electron microscopy are usually needed to firmly establish the diagnosis, cytology is the most readily available and rapid means of establishing a presumptive diagnosis of human polyomavirus infection. A urine specimen of a 24-year-old man with hemorrhagic cystitis beginning two months after bone marrow transplantation for acute myeloblastic leukemia(M2) was submitted for cytologic evaluation. Cytologic findings revealed a few inclusion-bearing epithelial cells intermingled with erythrocytes, neutrophils, lymphocytes, and macrophages. Most of the inclusion-bearing -cells had large, round to ovoid nuclei almost completely filed with homogeneous dark, basophilic inclusion. The chromatin was clumped along the periphery and the cytoplasm was mostly degenerated. The other cells exhibited irregular inclusions attached to the nuclear membrane surrounded by an indistinct halo. These findings were consistent with polyomavirus infection.

First
Prev
Page of 1
Next
Last

Search