Journal of Pathology and Translational Medicine

Original Articles

Characteristic Changes in Decidual Gene Expression Signature in Spontaneous Term Parturition: Haidy El-Azzamy, Andrea Balogh, Roberto Romero, Yi Xu, Christopher LaJeunesse, Olesya Plazyo, Zhonghui Xu, Theodore G. Price, Zhong Dong, Adi L. Tarca, Zoltan Papp, Sonia S. Hassan, Tinnakorn Chaiworapongsa, Chong Jai Kim, Nardhy Gomez-Lopez, Nandor Gabor Than; J Pathol Transl Med. 2017;51(3):264-283. Published online February 22, 2017; DOI: https://doi.org/10.4132/jptm.2016.12.20

13,507 View
274 Download
29 Web of Science
27 Crossref

Background
The decidua has been implicated in the “terminal pathway” of human term parturition, which is characterized by the activation of pro-inflammatory pathways in gestational tissues. However, the transcriptomic changes in the decidua leading to terminal pathway activation have not been systematically explored. This study aimed to compare the decidual expression of developmental signaling and inflammation-related genes before and after spontaneous term labor in order to reveal their involvement in this process.
Methods
Chorioamniotic membranes were obtained from normal pregnant women who delivered at term with spontaneous labor (TIL, n = 14) or without labor (TNL, n = 15). Decidual cells were isolated from snap-frozen chorioamniotic membranes with laser microdissection. The expression of 46 genes involved in decidual development, sex steroid and prostaglandin signaling, as well as pro- and anti-inflammatory pathways, was analyzed using high-throughput quantitative real-time polymerase chain reaction (qRT-PCR). Chorioamniotic membrane sections were immunostained and then semi-quantified for five proteins, and immunoassays for three chemokines were performed on maternal plasma samples.
Results
The genes with the highest expression in the decidua at term gestation included insulin-like growth factor-binding protein 1 (IGFBP1), galectin-1 (LGALS1), and progestogen-associated endometrial protein (PAEP); the expression of estrogen receptor 1 (ESR1), homeobox A11 (HOXA11), interleukin 1β (IL1B), IL8, progesterone receptor membrane component 2 (PGRMC2), and prostaglandin E synthase (PTGES) was higher in TIL than in TNL cases; the expression of chemokine C-C motif ligand 2 (CCL2), CCL5, LGALS1, LGALS3, and PAEP was lower in TIL than in TNL cases; immunostaining confirmed qRT-PCR data for IL-8, CCL2, galectin-1, galectin-3, and PAEP; and no correlations between the decidual gene expression and the maternal plasma protein concentrations of CCL2, CCL5, and IL-8 were found.
Conclusions
Our data suggests that with the initiation of parturition, the decidual expression of anti-inflammatory mediators decreases, while the expression of pro-inflammatory mediators and steroid receptors increases. This shift may affect downstream signaling pathways that can lead to parturition.

Citations

Citations to this article as recorded by

Progesterone inactivation in decidual stromal cells: A mechanism for inflammation-induced parturition
Angela DeTomaso, Hyeyon Kim, Jacqueline Shauh, Anika Adulla, Sarah Zigo, Maya Ghoul, Pietro Presicce, Suhas G. Kallapur, Wendy Goodman, Tamara Tilburgs, Sing-Sing Way, David Hackney, John Moore, Sam Mesiano
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef
The endoplasmic reticulum protein HSPA5/BiP is essential for decidual transformation of human endometrial stromal cells
Laura Fernández, Chow-Seng Kong, Majd Alkhoury, Maria Tryfonos, Paul J. Brighton, Thomas M. Rawlings, Joanne Muter, Maria Soledad Gori, Claudia Pérez Leirós, Emma S. Lucas, Jan J. Brosens, Rosanna Ramhorst
Scientific Reports.2024;[Epub] CrossRef
Is human labor at term an inflammatory condition?
Chandrashekara Kyathanahalli, Madeline Snedden, Emmet Hirsch
Biology of Reproduction.2023; 108(1): 23. CrossRef
CCL2: An important cytokine in normal and pathological pregnancies: A review
Zhi Lin, Jia-Lu Shi, Min Chen, Zi-Meng Zheng, Ming-Qing Li, Jun Shao
Frontiers in Immunology.2023;[Epub] CrossRef
Proteomic profile of extracellular vesicles in maternal plasma of women with fetal death
Dahiana M. Gallo, Wendy Fitzgerald, Roberto Romero, Nardhy Gomez-Lopez, Dereje W. Gudicha, Nándor Gábor Than, Mariachiara Bosco, Tinnakorn Chaiworapongsa, Eunjung Jung, Arun Meyyazhagan, Manaphat Suksai, Francesca Gotsch, Offer Erez, Adi L. Tarca, Leonid
The Journal of Maternal-Fetal & Neonatal Medicine.2023;[Epub] CrossRef
Downregulated INHBB in endometrial tissue of recurrent implantation failure patients impeded decidualization through the ADCY1/cAMP signalling pathway
Hui Zhang, Zhilong Wang, Quan Zhou, Zhiwen Cao, Yue Jiang, Manlin Xu, Jingyu Liu, Jidong Zhou, Guijun Yan, Haixiang Sun
Journal of Assisted Reproduction and Genetics.2023; 40(5): 1135. CrossRef
Regulation of inflammatory genes in decidual cells: Involvement of the bromodomain and extra-terminal family proteins
Sandeep Ajgaonkar, Jonathan J. Hirst, Mary Norris, Tamas Zakar, Giovanni Camussi
PLOS ONE.2023; 18(3): e0280645. CrossRef
Decidual cells and decidualization in the carnivoran endotheliochorial placenta
Mónica Elizabeth Diessler, Rocío Hernández, Gimena Gomez Castro, Claudio Gustavo Barbeito
Frontiers in Cell and Developmental Biology.2023;[Epub] CrossRef
Further Evidence that an Episode of Premature Labor Is a Pathologic State: Involvement of the Insulin-Like Growth Factor System
Priya Prasad, Roberto Romero, Tinnakorn Chaiworapongsa, Nardhy Gomez-Lopez, Anderson Lo, Jose Galaz, Andreea B. Taran, Eunjung Jung, Francesca Gotsch, Nandor Gabor Than, Adi L. Tarca
Fetal Diagnosis and Therapy.2023; 50(4): 236. CrossRef
Galectins-1, -3 and -9 Are Present in Breast Milk and Have a Role in Early Life Development
Karla Rio-Aige, Marina Girbal, Marta Selma-Royo, Anna Parra-Llorca, Sonia González, Cecilia Martínez-Costa, Margarida Castell, María Carmen Collado, Francisco J. Pérez-Cano, María J. Rodríguez-Lagunas
Nutrients.2022; 14(20): 4338. CrossRef
The Expression of IL-1β Correlates with the Expression of Galectin-3 in the Tissue at the Maternal–Fetal Interface during the Term and Preterm Labor
Nikola Jovic, Marija Milovanovic, Jovana Joksimovic Jovic, Marija Bicanin Ilic, Dejana Rakic, Vladimir Milenkovic, Bojana Stojanovic, Jelena Milovanovic, Aleksandar Arsenijevic, Nebojsa Arsenijevic, Mirjana Varjacic
Journal of Clinical Medicine.2022; 11(21): 6521. CrossRef
Galectin-1 and Galectin-9 Concentration in Maternal Serum: Implications in Pregnancies Complicated with Preterm Prelabor Rupture of Membranes
Dorota Grażyna Boroń, Aleksy Świetlicki, Michał Potograbski, Grażyna Kurzawińska, Przemysław Wirstlein, Daniel Boroń, Krzysztof Drews, Agnieszka Seremak-Mrozikiewicz
Journal of Clinical Medicine.2022; 11(21): 6330. CrossRef
Placental galectins regulate innate and adaptive immune responses in pregnancy
Orsolya Oravecz, Roberto Romero, Eszter Tóth, Judit Kapitány, Máté Posta, Dahiana M. Gallo, Simona W. Rossi, Adi L. Tarca, Offer Erez, Zoltán Papp, János Matkó, Nándor Gábor Than, Andrea Balogh
Frontiers in Immunology.2022;[Epub] CrossRef
Galectin-Levels Are Elevated in Infants Born Preterm Due to Amniotic Infection and Rapidly Decline in the Neonatal Period
Kirstin Faust, Nancy Freitag, Gabriela Barrientos, Christoph Hartel, Sandra M. Blois
Frontiers in Immunology.2021;[Epub] CrossRef
Inflammatory Amplification: A Central Tenet of Uterine Transition for Labor
Kelycia B. Leimert, Wendy Xu, Magdalena M. Princ, Sylvain Chemtob, David M. Olson
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Medawar’s PostEra: Galectins Emerged as Key Players During Fetal-Maternal Glycoimmune Adaptation
Ellen Menkhorst, Nandor Gabor Than, Udo Jeschke, Gabriela Barrientos, Laszlo Szereday, Gabriela Dveksler, Sandra M. Blois
Frontiers in Immunology.2021;[Epub] CrossRef
The Role of Decidual Subpopulations in Implantation, Menstruation and Miscarriage
Joanne Muter, Chow-Seng Kong, Jan J. Brosens
Frontiers in Reproductive Health.2021;[Epub] CrossRef
Multiomic immune clockworks of pregnancy
Laura S. Peterson, Ina A. Stelzer, Amy S. Tsai, Mohammad S. Ghaemi, Xiaoyuan Han, Kazuo Ando, Virginia D. Winn, Nadine R. Martinez, Kevin Contrepois, Mira N. Moufarrej, Stephen Quake, David A. Relman, Michael P. Snyder, Gary M. Shaw, David K. Stevenson, R
Seminars in Immunopathology.2020; 42(4): 397. CrossRef
Role of galectin-glycan circuits in reproduction: from healthy pregnancy to preterm birth (PTB)
Sandra M. Blois, Stefan Verlohren, Gang Wu, Gary Clark, Anne Dell, Stuart M. Haslam, Gabriela Barrientos
Seminars in Immunopathology.2020; 42(4): 469. CrossRef
Functional changes in decidual mesenchymal stem/stromal cells are associated with spontaneous onset of labour
Joan C Wijaya, Ramin Khanabdali, Harry M Georgiou, Maria I Kokkinos, Patrick F James, Shaun P Brennecke, Bill Kalionis
Molecular Human Reproduction.2020; 26(8): 636. CrossRef
Ageing in human parturition: impetus of the gestation clock in the decidua†
Joan C Wijaya, Ramin Khanabdali, Harry M Georgiou, Bill Kalionis
Biology of Reproduction.2020; 103(4): 695. CrossRef
Cytotrophoblast extracellular vesicles enhance decidual cell secretion of immune modulators via TNF-alpha
Sara K. Taylor, Sahar Houshdaran, Joshua F. Robinson, Matthew J. Gormley, Elaine Y. Kwan, Mirhan Kapidzic, Birgit Schilling, Linda C. Giudice, Susan J. Fisher
Development.2020;[Epub] CrossRef
A trypsin-based method for isolating leukocytes from human choriodecidua suitable for immunophenotyping and transcriptome studies
Karla MacDonald-Ramos, Marcia Arenas-Hernandez, Ismael Mancilla-Herrera, Claudia Rangel-Escareño, Rodrigo Vega-Sanchez
Immunobiology.2019; 224(1): 177. CrossRef
Transcriptomic analysis of fetal membranes reveals pathways involved in preterm birth
Silvana Pereyra, Claudio Sosa, Bernardo Bertoni, Rossana Sapiro
BMC Medical Genomics.2019;[Epub] CrossRef
Placental Galectins Are Key Players in Regulating the Maternal Adaptive Immune Response
Andrea Balogh, Eszter Toth, Roberto Romero, Katalin Parej, Diana Csala, Nikolett L. Szenasi, Istvan Hajdu, Kata Juhasz, Arpad F. Kovacs, Hamutal Meiri, Petronella Hupuczi, Adi L. Tarca, Sonia S. Hassan, Offer Erez, Peter Zavodszky, Janos Matko, Zoltan Pap
Frontiers in Immunology.2019;[Epub] CrossRef
Amnion epithelial cell–derived exosomes induce inflammatory changes in uterine cells
Emily E. Hadley, Samantha Sheller-Miller, George Saade, Carlos Salomon, Sam Mesiano, Robert N. Taylor, Brandie D. Taylor, Ramkumar Menon
American Journal of Obstetrics and Gynecology.2018; 219(5): 478.e1. CrossRef
Selective immuno-modulatory effect of prolactin upon pro-inflammatory response in human fetal membranes
Pilar Flores-Espinosa, Eduardo Preciado-Martínez, Araceli Mejía-Salvador, Gabriela Sedano-González, Luisa Bermejo-Martínez, Adalberto Parra-Covarruvias, Guadalupe Estrada-Gutiérrez, Rodrigo Vega-Sánchez, Isabel Méndez, Braulio Quesada-Reyna, Andrea Olmos-
Journal of Reproductive Immunology.2017; 123: 58. CrossRef

Do Helper T Cell Subtypes in Lymphocytic Thyroiditis Play a Role in the Antitumor Effect?: Seok Woo Yang, Seong-Ho Kang, Kyung Rae Kim, In Hong Choi, Hang Seok Chang, Young Lyun Oh, Soon Won Hong; J Pathol Transl Med. 2016;50(5):377-384. Published online September 15, 2016; DOI: https://doi.org/10.4132/jptm.2016.07.25

7,959 View
108 Download
2 Web of Science
3 Crossref

Abstract PDF

Background
Papillary thyroid carcinoma (PTC) is frequently accompanied by lymphocytic thyroiditis (LT). Some reports claim that Hashimoto’s thyroiditis (the clinical form of LT) enhances the likelihood of PTC; however, others suggest that LT has antitumor activity. This study was aimed to find out the relationship between the patterns of helper T cell (Th) cytokines in thyroid tissue of PTC with or without LT and the clinicopathological manifestation of PTC.
Methods
Fresh surgical samples of PTC with (13 cases) or without (10 cases) LT were used. The prognostic parameters (tumor size, extra-thyroidal extension of PTC, and lymph node metastasis) were analyzed. The mRNA levels of two subtypes of Th cytokines, Th1 (tumor necrosis factor α [TNF-α], interferon γ [IFN-γ ], and interleukin [IL] 2) and Th2 (IL-4 and IL-10), were analyzed. Because most PTC cases were microcarcinomas and recent cases without clinical follow-up, negative or faint p27 immunoreactivity was used as a surrogate marker for lymph node metastasis.
Results
PTC with LT cases showed significantly higher expression of TNF-α (p = .043), IFN-γ (p < .010), IL-4 (p = .015) than those without LT cases. Although the data were not statistically significant, all analyzed cytokines (except for IL-4) were highly expressed in the cases with higher expression of p27 surrogate marker.
Conclusions
These results indicate that mixed Th1 (TNF-α, IFN-γ , and IL-2) and Th2 (IL-10) immunity might play a role in the antitumor effect in terms of lymph node metastasis.

Citations

Citations to this article as recorded by

Obesity and Thyroid Cancer Risk: An Update
Fabiana Franchini, Giuseppe Palatucci, Annamaria Colao, Paola Ungaro, Paolo Emidio Macchia, Immacolata Cristina Nettore
International Journal of Environmental Research and Public Health.2022; 19(3): 1116. CrossRef
Association between Hashimoto thyroiditis and clinical outcomes of papillary thyroid carcinoma: A meta-analysis
Qizhi Tang, Weiyu Pan, Liangyue Peng, Francis Moore
PLOS ONE.2022; 17(6): e0269995. CrossRef
The Heat Shock Protein Story—From Taking mTORC1,2 and Heat Shock Protein Inhibitors as Therapeutic Measures for Treating Cancers to Development of Cancer Vaccines
Peter Chin Wan Fung, Regina Kit Chee Kong
Journal of Cancer Therapy.2017; 08(11): 962. CrossRef

A Study on the Tumor Angiogenesis and Expression of Cytokine and Growth Factors in the Prostatic Carcinoma.: Sung Chul Lim, Ho Jong Jeon; Korean J Pathol. 1996;30(8):671-679.

1,470 View
16 Download

Abstract PDF: There is considerable experimental evidence to indicate that tumor growth is dependent on angiogenesis. However, we do not understand how the angiogenic activity is initiated by a given tumor. There is a clear distinction between a stage without neovascularization, which correlates with a paucity of metastases, and a stage in which increasing neovascularization correlates with a rising rate of metastasis. The authors therefore asked whether the extent of angiogenesis in human prostatic carcinoma is correlated with the tumor grades or some growth factors. To investigate how tumor angiogenesis correlates with tumor aggressiveness, the authors counted microvessels within the various grades of invasive prostatic carcinomas of 44 patients and the nodular hyperplasias of 10 patients. Highlighting of the vessels by immunohistochemical staining for factor VIII-related antigen and assessment of the tumor aggressiveness by the degree of expression of some growth factors(transforming growth factor-alpha, and beta, epidermal growth factor), tumor necrosis factor-alpha and tumor grading(Gleason's score) were done. As a result, both microvessel counts and the expression of growth factors and tumor necrosis factor correlated with tumor grades. In conclusion, the number of microvessels per 200 X fields in the areas of most intense neovascularization in a prostatic carcinoma may be a predictor of the patient's prognosis. Therefore, assessment of tumor angiogenesis may prove valuable in selecting patients with prostatic carcinoma, especially small needle biopsy, for aggressive therapy.

Cytokine Expression on Microglial Proliferation and Apoptosis in Rat Lumbar Spinal Cord Following Unilateral Sciatic Nerve Transection.: Sang Pyo Kim, Seung Il Suh, Young Rok Cho, Seung Che Cho, Seung Pil Kim, Jong Wook Park, Jyung Sik Kwak; Korean J Pathol. 1998;32(2):94-103.

1,612 View
16 Download

Abstract PDF: This study was carried out to elucidate the cytokine mRNAs expression and morphological features according to a microglial proliferation and apoptosis in a rat lumbar spinal cord, after a right sciatic nerve transection. The control group was composed of 5 rats (Spraque-Dawley) and the experimental group was composed of 70 rats. On post operation day (pod) 1, 2, 3, 5, and 7 eight rats were sacrificed on those days. On pod 10 five rats were sacrificed as well as five rats sacrificed on post operation weeks 2, 3, 4, 5, and 6. On light microscopy, activated microglia were often found in a perineuronal position around motoneurons in the ventral gray matter and more randomly distributed throughout the neuropil in the dorsal gray matter of lumbar spinal cord. GSA I-B4-positive microglia began to increase from 1 day after transection, and reached peak at 2~3 days and it persisted at 5~7 days and decreased thereafter. TUNEL-positive microglia was not observed in control group and began to increase from 5 days after transection and increased gradually until 3 weeks and decreased thereafter. On in situ RT-PCR, the positive signal for IL-1alpha and IL-6 mRNA was found mainly in the cytoplasm of the activated microglia and astrocytes at 1 day after transection and showed stronger signal at 3 days and decreased gradually until 10 days. TNF-alpha mRNA was detected 1 day after transection and remained for 7 days and localized to activated microglia as well as probably some astrocytes. The signal intensity of IL-1alpha and IL-6 mRNA was generally stronger than TNF-alpha mRNA. On transmission electron microscopy, there were chromatin condensation with margination toward nuclear membrane and condensation of cytoplasm at 3 days after transection. Apoptotic bodies were found after 5 days and increased gradually until 3 weeks. According to the above findings, it is concluded that apoptosis appears to be one mechanism by which activated microglia are gradually eliminated and cytokine expression seems to played an active role in the microglial turnover.

Pathologic Analysis of Endomyocardial Biopsies in Heart Transplantation.: Mee Hye Oh, Jeong Wook Seo, Kook Yang Park, Young Tak Lee, Yoon Seop Jeong, Suk Keun Hong, Joon Ryang Rho, Byung Hee Oh, Sung Sook Kim; Korean J Pathol. 1998;32(2):104-114.

1,667 View
14 Download

Abstract PDF: Endomyocardial biopsy (EMB) is a valuable diagnostic procedure for the surveillance of cardiac allograft rejection. Interpretation of individual cases is still problematic due to variations of findings for grading of rejection and other associated lesions. We reevaluated an experience on endomyocardial biopsies to develop better diagnostic criteria for rejection and other complications. Immunohistochemical studies against cytokines were performed to assess the usefulness of the method for the diagnosis or researches. A total of 249 EMBs taken from 33 cardiac allograft recipients were reviewed. There were 25 males and 8 females. Dilated cardiomyopathy was present (24 cases) and valvular heart disease (4 cases), restrictive cardiomyopathy (3 cases) were also common conditions. We applied the grading system of the International Society for Heart Transplantation (ISHT) for the assessment of acute cellular rejection. Grades of 0, 1A, 1B, 2, 3A and 3B were 39.0%, 28.1%, 11.2%, 11.5%, 12.4% and 1.6% respectively, but 3.2% were inadequate. Thirty five episodes of grade 3A or 3B were present in 17 patients. The response to therapy was assessed using a next follow up biopsy, which revealed resolving or resolved rejection in 85% of patients. The intensity of immunohistochemical stains for IL-6 and TNF-alpha was increased in proportion to the histologic grade but Quilty lesion and cardiomyopathy also showed a positive reaction. The other pathologic findings were ischemic change, previous biopsy site, interstitial edema and fibrosis, and Quilty lesion. These findings showed usefulness of endomyocardial biopsy not only for the evaluation of cardiac allograft rejection but also for the diagnosis of associated cardiac lesions. Immunohistochemical study of the cytokines was related to the degree of inflammation rather than degree of rejection.

Intrauterine Infection as a Cause of the Neonatal Pulmonary Injury and Bronchopulmonary Dysplasia.: Jin Haeng Chung, Jeong Wook Seo; Korean J Pathol. 2000;34(6):431-436.

1,439 View
12 Download

Abstract PDF: The pathogenetic role of intrauterine infection to the neonatal pulmonary injury and bronchopulmonary dysplasia was assessed by studying the interleukin-6 (IL-6) level in the umbilical cord blood and the early morphologic changes of the neonatal lung. Patients were grouped into bronchopulmonary dysplasia (4 cases), chorioamnionitis without chronic lung injury (4 cases), and 6 cases without morphologic evidence of chronic lung injury or placental inflammation. IL-6 level of umbilical cord blood was higher in babies with bronchopulmonary dysplasia (17.7 pg/ml) compared to those with chorioamnionitis (4.7 pg/ml) or those with morphologically normal lung and placenta (6.2 pg/ml). Morphologic parameters of neonatal pulmonary injury were hyaline membrane, terminal bronchiole inflammation, terminal bronchiole regeneration, alveolar collapse and fibroblastic proliferation. Bronchiolar regeneration was the most peculiar feature seen in the lung with bronchopulmonary dysplasia. Alveolar collapse and interstitial fibroblastic reaction were commonly seen in bronchopulmonary dysplasia. The postnatal age at death was higher in those with bronchopulmonary dysplasia, although the occurrence of the morphologic changes was related with the chronicity of those lesions. These findings suggest that intrauterine infection is an aggravating factor for the neonatal pulmonary injury and bronchopulmonary dysplasia, although the early stage of the lung injury is not a definitive indicator for the progressive pulmonary damage leading to the bronchopulmonary dysplasia.

First
Prev
Page of 1
Next
Last

Search