Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication.
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Ginkgo biloba Extract Attenuates Methotrexate-Induced Testicular Injury in Rats: Cross-talk Between Oxidative Stress, Inflammation, Apoptosis, and miRNA-29a Expression Iman O. Sherif, Laila A. Al-Mutabagani, Osama M. Sarhan Integrative Cancer Therapies.2020;[Epub] CrossRef
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Tumor-Associated Protein Profiles in Kaposi Sarcoma and Mimicking Vascular Tumors, and Their Pathological Implications Kyoung Bun Lee, Kyu Sang Lee, Hye Seung Lee International Journal of Molecular Sciences.2019; 20(13): 3142. CrossRef
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Pathology Services in Nigeria: Cross-Sectional Survey Results From Three Cancer Consortia Peter Ntiamoah, Ngozi R. Monu, Fatimah B. Abdulkareem, Kayode A. Adeniji, John O. Obafunwa, Akinwumi O. Komolafe, Clayton Yates, Ernest Kaninjing, John D. Carpten, Bodour Salhia, Folake T. Odedina, Marcia Edelweiss, T. Peter Kingham, Olusegun I. Alatise Journal of Global Oncology.2019; (5): 1. CrossRef
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Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring Michelle A. Wood-Trageser, Andrew J. Lesniak, Anthony J. Demetris Transplantation.2019; 103(7): 1306. CrossRef
Anything You Can Do, I Can Do Better: Can Aptamers Replace Antibodies in Clinical Diagnostic Applications? Michelle Bauer, Mia Strom, David S Hammond, Sarah Shigdar Molecules.2019; 24(23): 4377. CrossRef
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Update on Immunohistochemistry for the Diagnosis of Lung Cancer Kentaro Inamura Cancers.2018; 10(3): 72. CrossRef
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