Skip Navigation
Skip to contents

J Pathol Transl Med : Journal of Pathology and Translational Medicine

OPEN ACCESS
SEARCH
Search

Search

Page Path
HOME > Search
1 "RDO GOLD"
Filter
Filter
Article category
Keywords
Publication year
Authors
Original Article
Proposal of an Appropriate Decalcification Method of Bone Marrow Biopsy Specimens in the Era of Expanding Genetic Molecular Study
Sung-Eun Choi, Soon Won Hong, Sun Och Yoon
J Pathol Transl Med. 2015;49(3):236-242.   Published online May 15, 2015
DOI: https://doi.org/10.4132/jptm.2015.03.16
  • 13,616 View
  • 291 Download
  • 45 Web of Science
  • 47 Crossref
AbstractAbstract PDF
Background
The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material is necessary. Methods: Pairs of bilateral bone marrow biopsies sampled from 53 patients were decalcified according to protocols of two comparison groups: EDTA versus HCl and RDO GOLD (RDO) versus HCl. Pairs of right and left bone marrow biopsy samples harvested from 28 cases were allocated into the EDTA versus HCl comparison group, and 25 cases to the RDO versus HCl comparison group. The decalcification protocols were compared with regards to histomorphology, immunohistochemistry, and molecular analysis. For molecular analysis, we randomly selected 5 cases from the EDTA versus HCl and RDO versus HCl groups. Results: The decalcification time for appropriate histomorphologic analysis was the longest in the EDTA method and the shortest in the RDO method. EDTA was superior to RDO or HCl in DNA yield and integrity, assessed via DNA extraction, polymerase chain reaction, and silver in situ hybridization using DNA probes. The EDTA method maintained intact nuclear protein staining on immunohistochemistry, while the HCl method produced poor quality images. Staining after the RDO method had equivocal results. RNA in situ hybridization using kappa and lambda RNA probes measured RNA integrity; the EDTA and RDO method had the best quality, followed by HCl. Conclusions: The EDTA protocol would be the best in preserving genetic material. RDO may be an acceptable alternative when rapid decalcification is necessary.

Citations

Citations to this article as recorded by  
  • Optimization of Formic Acid-Formalin-Based Decalcification Protocol for Rat Calvarial Bone Histology
    S. Amitha Banu, Khan Sharun, Merlin Mamachan, Athira Subash, Vadapalli Deekshita, Kirtika Sharma, Karikalan Mathesh, Obli Rajendran Vinodh kumar, Swapan Kumar Maiti, Abhijit M. Pawde, Laith Abualigah, Kuldeep Dhama, Amarpal
    Journal of Experimental Biology and Agricultural Sciences.2024; 12(2): 218.     CrossRef
  • Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow
    Gabriella M. L. Diamantino, Janet Beeler-Marfisi, Robert A. Foster, William Sears, Alice Defarges, William Vernau, Dorothee Bienzle
    Veterinary Pathology.2024; 61(6): 943.     CrossRef
  • In situ metabolomic analysis of osteonecrosis of the femoral head (ONFH) using MALDI MSI
    Chen Li, Jikun Liu, Yiqi Sheng, Yinghao Wang, Lan Jia, Yinguang Zhang, Jiantao Li, Shuangshuang Di, Honggang Nie, Yehua Han
    Analytical and Bioanalytical Chemistry.2024; 416(23): 5155.     CrossRef
  • A set of pretreatment reagents including improved formula fixation and decalcification facilitating immunohistochemistry and DNA analyses of formalin-fixed paraffin-embedded bone marrow trephine biopsy
    Ting Sun, Liming Xu, Hongtian Yao, Jing Zhao, Zhen Chen, Zexin Chen, Bo Wang, Wei Ding
    Acta Histochemica.2024; 126(8): 152188.     CrossRef
  • Germline and somatic testing for homologous repair deficiency in patients with prostate cancer (part 1 of 2)
    Andrew J. Armstrong, Amy Taylor, Michael C. Haffner, Wassim Abida, Alan H. Bryce, Lawrence I. Karsh, Scott T. Tagawa, Przemyslaw Twardowski, Anthony V. Serritella, Joshua M. Lang
    Prostate Cancer and Prostatic Diseases.2024;[Epub]     CrossRef
  • To Freeze or Not to Freeze? Recommendations for Intraoperative Examination and Gross Prosection of Thyroid Glands
    Fouad R. Zakka, Nicole A. Cipriani
    Surgical Pathology Clinics.2023; 16(1): 15.     CrossRef
  • Effect of Surface Decalcification With Hydrochloric Acid on the Determination of Estrogen Receptor, Progesterone Receptor, Ki67, and Human Epidermal Growth Factor Receptor 2 Expressions in Invasive Breast Carcinoma Based on Immunohistochemistry and Fluore
    Wu Ping, Rao Xin, Zhang Li, Chen Yupeng, Song Fangling, Ren Caihong, Hu Shun, Zhang Sheng
    Applied Immunohistochemistry & Molecular Morphology.2023; 31(4): 232.     CrossRef
  • Diagnostic value of MDM2 RNA in situ hybridization for low-grade osteosarcoma: Consistency comparison of RNA in situ hybridization, fluorescence in situ hybridization, and immunohistochemistry
    Chen Chen, Xin He, Min Chen, Tianhai Du, Weiji Qin, Wenyi Jing, Hongying Zhang
    Virchows Archiv.2023; 482(6): 999.     CrossRef
  • Bone marrow fibrosis is associated with non‐response to CD19 CAR T‐cell therapy in B‐acute lymphoblastic leukemia
    Joshua Anil, Ahab Alnemri, Andrew Lytle, Brian Lockhart, Ashley E. Anil, Michael Baumgartner, Kirubel Gebre, Jared McFerran, Stephan A. Grupp, Susan R. Rheingold, Vinodh Pillai
    American Journal of Hematology.2023; 98(12): 1888.     CrossRef
  • Epithelioid haemangioendothelioma of the mandible – A case report and review of the literature
    Ali Rizvi, Tim K. Blackburn, Guy N. J. Betts
    Oral Surgery.2022; 15(3): 387.     CrossRef
  • Evaluation of EDTA and nitric acid solutions for decalcification of joints in AG/WT, BALB/c, C57, DBA1/J mice, and in Wistar rats
    Eduarda Correa Freitas, Suelen Pizzolatto Dalmolin, Mateus Müller da Silva, Francine Hehn de Oliveira, Emily Ferreira Salles Pilar
    Biotechnic & Histochemistry.2022; 97(5): 372.     CrossRef
  • Coupling Lipid Labeling and Click Chemistry Enables Isolation of Extracellular Vesicles for Noninvasive Detection of Oncogenic Gene Alterations
    Na Sun, Benjamin V. Tran, Zishan Peng, Jing Wang, Ceng Zhang, Peng Yang, Tiffany X. Zhang, Josephine Widjaja, Ryan Y. Zhang, Wenxi Xia, Alexandra Keir, Jia‐Wei She, Hsiao‐hua Yu, Jing‐Jong Shyue, Hongguang Zhu, Vatche G. Agopian, Renjun Pei, James S. Toml
    Advanced Science.2022;[Epub]     CrossRef
  • The Expressions of CD30 and CD123 of Mastocytosis in Taiwan
    Ching-Fen Yang, Chih-Yi Hsu
    Applied Immunohistochemistry & Molecular Morphology.2022; 30(4): 278.     CrossRef
  • Unusual Patterns of HER2 Expression in Breast Cancer: Insights and Perspectives
    Dora Grassini, Eliano Cascardi, Ivana Sarotto, Laura Annaratone, Anna Sapino, Enrico Berrino, Caterina Marchiò
    Pathobiology.2022; 89(5): 278.     CrossRef
  • Expert opinion on NSCLC small specimen biomarker testing — Part 1: Tissue collection and management
    Frédérique Penault-Llorca, Keith M. Kerr, Pilar Garrido, Erik Thunnissen, Elisabeth Dequeker, Nicola Normanno, Simon J. Patton, Jenni Fairley, Joshua Kapp, Daniëlle de Ridder, Aleš Ryška, Holger Moch
    Virchows Archiv.2022; 481(3): 335.     CrossRef
  • Comparison of bone demineralisation procedures for DNA recovery from burned remains
    Meghan Mckinnon, Denice Higgins
    Forensic Science International: Genetics.2021; 51: 102448.     CrossRef
  • A review of the current understanding of burned bone as a source of DNA for human identification
    Meghan Mckinnon, Maciej Henneberg, Denice Higgins
    Science & Justice.2021; 61(4): 332.     CrossRef
  • Time is bone — Quantitative comparison of decalcification solvents in human femur samples using dual-X-ray-absorptiometry and computed tomography
    Joshua Gawlitza, Jakob Steinhäuser, Arno Bücker, Gabriela Krasteva-Christ, Thomas Tschernig
    Annals of Anatomy - Anatomischer Anzeiger.2021; 235: 151696.     CrossRef
  • Molecular biomarker testing for non–small cell lung cancer: consensus statement of the Korean Cardiopulmonary Pathology Study Group
    Sunhee Chang, Hyo Sup Shim, Tae Jung Kim, Yoon-La Choi, Wan Seop Kim, Dong Hoon Shin, Lucia Kim, Heae Surng Park, Geon Kook Lee, Chang Hun Lee
    Journal of Pathology and Translational Medicine.2021; 55(3): 181.     CrossRef
  • Effect of EDTA decalcification on estrogen receptor and progesterone receptor immunohistochemistry and HER2/neu fluorescence in situ hybridization in breast carcinoma
    Erik Washburn, Xiaoyu Tang, Carla Caruso, Michelle Walls, Bing Han
    Human Pathology.2021; 117: 108.     CrossRef
  • Performances of single tube nested polymerase chain reaction and GeneXpert ultra on Formalin fixed paraffin embedded tissues in the diagnosis of tuberculous spondylodiscitis
    Emna Romdhane, Soumaya Rammeh, Chelli Mouna Bouaziz, Hend Riahi, Meriam Rekaya Ben, Meriam Ksentini, Yosra Chebbi, Wafa Achour, Asma Ferjani, Ben Boubaker Ilhem Boutiba, Leila Slim-Saidi, Mohamed Fethi Ladeb
    Clinical Rheumatology.2021; 40(10): 4317.     CrossRef
  • Molecular Characterization of Prostate Cancers in the Precision Medicine Era
    Emilio Francesco Giunta, Laura Annaratone, Enrico Bollito, Francesco Porpiglia, Matteo Cereda, Giuseppe Luigi Banna, Alessandra Mosca, Caterina Marchiò, Pasquale Rescigno
    Cancers.2021; 13(19): 4771.     CrossRef
  • Increased NF-κB Activity in Osteoprogenitor-Lineage Cells Impairs the Balance of Bone Versus Fat in the Marrow of Skeletally Mature Mice
    Tzuhua Lin, Jukka Pajarinen, Yusuke Kohno, Akira Nabeshima, Laura Lu, Karthik Nathan, Zhenyu Yao, Joy Y. Wu, Stuart Goodman
    Regenerative Engineering and Translational Medicine.2020; 6(1): 69.     CrossRef
  • Percutaneous CT-guided biopsy of lytic bone lesions in patients clinically suspected of lung cancer: Diagnostic performances for pathological diagnosis and molecular testing
    Anne-Claire Toffart, Stéphane Asfari, Anne Mc Leer, Emilie Reymond, Adrien Jankowski, Denis Moro-Sibilot, Olivier Stephanov, Julien Ghelfi, Sylvie Lantuejoul, Gilbert R. Ferretti
    Lung Cancer.2020; 140: 93.     CrossRef
  • Effect of decalcification protocols on immunohistochemistry and molecular analyses of bone samples
    Elodie Miquelestorena-Standley, Marie-Lise Jourdan, Christine Collin, Corinne Bouvier, Frédérique Larousserie, Sébastien Aubert, Anne Gomez-Brouchet, Jean-Marc Guinebretière, Matthias Tallegas, Bénédicte Brulin, Louis-Romée Le Nail, Anne Tallet, François
    Modern Pathology.2020; 33(8): 1505.     CrossRef
  • Identifying Opportunities and Challenges for Patients With Sarcoma as a Result of Comprehensive Genomic Profiling of Sarcoma Specimens
    Margaret A. Hay, Eric A. Severson, Vincent A. Miller, David A. Liebner, Jo-Anne Vergilio, Sherri Z. Millis, James L. Chen
    JCO Precision Oncology.2020; (4): 176.     CrossRef
  • Comparison of Methods for the Histological Evaluation of Odontocete Spiral Ganglion Cells
    Tania Ramírez, Simona Sacchini, Yania Paz, Rubén S. Rosales, Nakita Câmara, Marisa Andrada, Manuel Arbelo, Antonio Fernández
    Animals.2020; 10(4): 683.     CrossRef
  • Molecular Pathology of Primary Non-small Cell Lung Cancer
    David Ilan Suster, Mari Mino-Kenudson
    Archives of Medical Research.2020; 51(8): 784.     CrossRef
  • Comparison of ethylenediaminetetraacetic acid and rapid decalcificier solution for studying human temporal bones by immunofluorescence
    Sumana Ghosh, Mark B. Lewis, Bradley J. Walters
    Laryngoscope Investigative Otolaryngology.2020; 5(5): 919.     CrossRef
  • A novel cryo-embedding method for in-depth analysis of craniofacial mini pig bone specimens
    Pavla Ticha, Igor Pilawski, Xue Yuan, Jie Pan, Ustun S. Tulu, Benjamin R. Coyac, Waldemar Hoffmann, Jill A. Helms
    Scientific Reports.2020;[Epub]     CrossRef
  • Accelerating precision medicine in metastatic prostate cancer
    Joaquin Mateo, Rana McKay, Wassim Abida, Rahul Aggarwal, Joshi Alumkal, Ajjai Alva, Felix Feng, Xin Gao, Julie Graff, Maha Hussain, Fatima Karzai, Bruce Montgomery, William Oh, Vaibhav Patel, Dana Rathkopf, Matthew Rettig, Nikolaus Schultz, Matthew Smith,
    Nature Cancer.2020; 1(11): 1041.     CrossRef
  • Tissue Morphology and Antigenicity in Mouse and Rat Tibia: Comparing 12 Different Decalcification Conditions
    Kristofor Bogoevski, Anna Woloszyk, Keith Blackwood, Maria A. Woodruff, Vaida Glatt
    Journal of Histochemistry & Cytochemistry.2019; 67(8): 545.     CrossRef
  • Cellular and collagen reference values of gingival and periodontal ligament tissues in rats: a pilot study
    Antoine Alves, Nina Attik, Carine Wirth, Yves Bayon, Alexis Piat, Brigitte Grosgogeat, Kerstin Gritsch
    Histochemistry and Cell Biology.2019; 152(2): 145.     CrossRef
  • Implementing Precision Medicine Programs and Clinical Trials in the Community-Based Oncology Practice: Barriers and Best Practices
    Jennifer L. Ersek, Lora J. Black, Michael A. Thompson, Edward S. Kim
    American Society of Clinical Oncology Educational Book.2018; (38): 188.     CrossRef
  • Integration of next-generation sequencing in clinical diagnostic molecular pathology laboratories for analysis of solid tumours; an expert opinion on behalf of IQN Path ASBL
    Zandra C Deans, Jose Luis Costa, Ian Cree, Els Dequeker, Anders Edsjö, Shirley Henderson, Michael Hummel, Marjolijn JL Ligtenberg, Marco Loddo, Jose Carlos Machado, Antonio Marchetti, Katherine Marquis, Joanne Mason, Nicola Normanno, Etienne Rouleau, Ed S
    Virchows Archiv.2017; 470(1): 5.     CrossRef
  • Protocolo para el estudio de muestras y estandarización del informe patológico de tumores óseos
    Isidro Machado, José Juan Pozo, David Marcilla, Julia Cruz, Juan C. Tardío, Aurora Astudillo, Sílvia Bagué
    Revista Española de Patología.2017; 50(1): 34.     CrossRef
  • Extremely Well-Differentiated Papillary Thyroid Carcinoma Resembling Adenomatous Hyperplasia Can Metastasize to the Skull: A Case Report
    Ju Yeon Pyo, Jisup Kim, Sung-eun Choi, Eunah Shin, Seok-Woo Yang, Cheong Soo Park, Seok-Mo Kim, SoonWon Hong
    Yonsei Medical Journal.2017; 58(1): 255.     CrossRef
  • Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species
    Guoying Deng, Kerun Niu, Feng Zhou, Buxiao Li, Yingjie Kang, Xijian Liu, Junqing Hu, Bo Li, Qiugen Wang, Chengqing Yi, Qian Wang
    Scientific Reports.2017;[Epub]     CrossRef
  • Precision Medicine Starts With Preanalytics: Real-Time Assessment of Tissue Fixation Quality by Ultrasound Time-of-Flight Analysis
    Melissa L. Lerch, Daniel R. Bauer, David Chafin, Abbey Theiss, Michael Otter, Geoffrey S. Baird
    Applied Immunohistochemistry & Molecular Morphology.2017; 25(3): 160.     CrossRef
  • Good Laboratory Standards for Clinical Next-Generation Sequencing Cancer Panel Tests
    Jihun Kim, Woong-Yang Park, Nayoung K. D. Kim, Se Jin Jang, Sung-Min Chun, Chang-Ohk Sung, Jene Choi, Young-Hyeh Ko, Yoon-La Choi, Hyo Sup Shim, Jae-Kyung Won
    Journal of Pathology and Translational Medicine.2017; 51(3): 191.     CrossRef
  • An international survey about nail histology processing techniques
    Christina Wlodek, Pauline Lecerf, Josette Andre, Beth S. Ruben, David de Berker
    Journal of Cutaneous Pathology.2017; 44(9): 749.     CrossRef
  • pSTAT5 and ERK exhibit different expression in myeloproliferative neoplasms
    Ewa Wiśniewska-Chudy, Łukasz Szylberg, Grzegorz Dworacki, Ewa Mizera-Nyczak, Andrzej Marszałek
    Oncology Reports.2017; 37(4): 2295.     CrossRef
  • How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days
    I. de Laak–de Vries, A. G. Siebers, L. Burgers, C. Diepenbroek, M. Link, P. Groenen, J. H. J. M. van Krieken, K. M. Hebeda
    Journal of Hematopathology.2016; 9(2): 67.     CrossRef
  • Do More With Less: Tips and Techniques for Maximizing Small Biopsy and Cytology Specimens for Molecular and Ancillary Testing: The University of Colorado Experience
    Dara L. Aisner, Mathew D. Rumery, Daniel T. Merrick, Kimi L. Kondo, Hala Nijmeh, Derek J. Linderman, Robert C. Doebele, Natalie Thomas, Patrick C. Chesnut, Marileila Varella-Garcia, Wilbur A. Franklin, D. Ross Camidge
    Archives of Pathology & Laboratory Medicine.2016; 140(11): 1206.     CrossRef
  • Analysis of the Effects of Bone Marrow Biopsy Decalcification Methods on Histopathological Examination
    Ji Young Park, Kyung Hee Han
    The Korean Journal of Clinical Laboratory Science.2016; 48(4): 371.     CrossRef
  • Distinguishing between Microbial Habitats Unravels Ecological Complexity in Coral Microbiomes
    Amy Apprill, Laura G. Weber, Alyson E. Santoro, Nicole S. Webster
    mSystems.2016;[Epub]     CrossRef
  • Optimal Fixation and Decalcification Methods for Bone Marrow Biopsy
    Myung-Sub Choi, Hyunsup Lee, Hyuk-Chul Kwon, Moon-Hwan Bae, Young-Hye Ko, Hee-Jin Kim, Beom-Se Lee, Bon-Kyung Koo
    Korean Journal of Clinical Laboratory Science.2015; 47(4): 243.     CrossRef

J Pathol Transl Med : Journal of Pathology and Translational Medicine
TOP