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Original Article
- Does Polymerase Chain Reaction of Tissue Specimens Aid in the Diagnosis of Tuberculosis?
- Yoo Jin Lee, Seojin Kim, Youngjin Kang, Jiyoon Jung, Eunjung Lee, Joo-Young Kim, Jeong Hyeon Lee, Youngseok Lee, Yang-seok Chae, Chul Hwan Kim
- J Pathol Transl Med. 2016;50(6):451-458. Published online October 10, 2016
- DOI: https://doi.org/10.4132/jptm.2016.08.04
- 9,901 View
- 240 Download
- 6 Web of Science
- 9 Crossref
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Abstract
PDF - Background
Mycobacterial culture is the gold standard test for diagnosing tuberculosis (TB), but it is time-consuming. Polymerase chain reaction (PCR) is a highly sensitive and specific method that can reduce the time required for diagnosis. The diagnostic efficacy of PCR differs, so this study determined the actual sensitivity of TB-PCR in tissue specimens.
Methods
We retrospectively reviewed 574 cases. The results of the nested PCR of the IS6110 gene, mycobacterial culture, TB-specific antigen-induced interferon-γ release assay (IGRA), acid-fast bacilli (AFB) staining, and histological findings were evaluated.
Results
The positivity rates were 17.6% for PCR, 3.3% for the AFB stain, 22.2% for mycobacterial culture, and 55.4% for IGRA. PCR had a low sensitivity (51.1%) and a high specificity (86.3%) based on the culture results of other studies. The sensitivity was higher (65.5%) in cases with necrotizing granuloma but showed the highest sensitivity (66.7%) in those with necrosis only. The concordance rate between the methods indicated that PCR was the best method compared to mycobacterial culture, and the concordance rate increased for the methods using positive result for PCR or histologic features.
Conclusions
PCR of tissue specimens is a good alternative to detect tuberculosis, but it may not be as sensitive as previously suggested. Its reliability may also be influenced by some histological features. Our data showed a higher sensitivity when specimens contained necrosis, which indicated that only specimens with necrosis should be used for PCR to detect tuberculosis. -
Citations
Citations to this article as recorded by
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The Need for Persistence in the Diagnosis of Mycobacterium Tuberculosis Mono-arthritis: A Unique Case Presentation
T. Bekoulis, P. Christodoulou, K. Dogramatzis, E. Markopoulou, Emmanouel Antonogiannakis, E. Kokkinakis, Alexandros P. Apostolopoulos, A. Manimanaki
Journal of Long-Term Effects of Medical Implants.2024; 34(1): 35. CrossRef - A Case Report on Scrofuloderma: A Cutaneous Manifestation of Tuberculosis
Soham R Meghe, Adarshlata Singh, Drishti M Bhatt, Shreya N Gupta, Varun Hanumanthaiah, Shree Ramya Talasila
Cureus.2024;[Epub] CrossRef - An overview of infectious disease laboratory methods: an update for the histopathologist
Daniel R. Stevenson
Diagnostic Histopathology.2024; 30(10): 534. CrossRef - Diagnostic Utility of Biplex/Multiplex Polymerase Chain Reaction in Infectious Granulomatous Dermatitis in North Indian Population
Mayur Parkhi, Mukin Kumar S, Dipankar De, Rakesh Yadav, Sunil Sethi, Bishan Dass Radotra, Uma Nahar Saikia
The American Journal of Dermatopathology.2021; 43(8): 567. CrossRef - Reduction of turnaround time for non-tuberculous mycobacteria detection in heater–cooler units by propidium monoazide–real-time polymerase chain reaction
S. Ditommaso, M. Giacomuzzi, G. Memoli, R. Cavallo, A. Curtoni, M. Avolio, C. Silvestre, C.M. Zotti
Journal of Hospital Infection.2020; 104(3): 365. CrossRef - Ergonomic Diagnostic Tool based on Chip Mini RT-PCR for Diagnosis of Pulmonary and Extra Pulmonary Tuberculosis
V Mangayarkarasi, Sneka P, Sujith R, Jayaprakash Jayaprakash
Journal of Pure and Applied Microbiology.2019; 13(2): 1185. CrossRef - Cutaneous Tuberculosis: Clinicopathologic Arrays and Diagnostic Challenges
Priyatam Khadka, Soniya Koirala, Januka Thapaliya
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Gastroenterology & Hepatology: Open Access.2017;[Epub] CrossRef
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The Need for Persistence in the Diagnosis of Mycobacterium Tuberculosis Mono-arthritis: A Unique Case Presentation
Brief Case Report
- Intramuscular Tenosynovial Giant Cell Tumor, Diffuse-Type
- Yoo Jin Lee, Youngjin Kang, Jiyoon Jung, Seojin Kim, Chul Hwan Kim
- J Pathol Transl Med. 2016;50(4):306-308. Published online January 11, 2016
- DOI: https://doi.org/10.4132/jptm.2015.11.15
- 8,400 View
- 112 Download
- 2 Web of Science
- 4 Crossref
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PDF
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Citations
Citations to this article as recorded by- Intramuscular Tenosynovial Giant Cell Tumor Harboring a Novel CSF1-CD96 Fusion Transcript
Haider Mejbel, Gene P. Siegal, Shi Wei
International Journal of Surgical Pathology.2022; 30(3): 335. CrossRef - Diffuse-Type Tenosynovial Giant Cell Tumor of the Tendon Sheath in Both Wrists
Sunah Heo, Sun-Young Park, Jinwon Seo, Sung Hye Koh, In Jae Lee
Journal of the Korean Society of Radiology.2021; 82(1): 250. CrossRef - Limited usefulness of classic MR findings in the diagnosis of tenosynovial giant cell tumor
Julia Crim, Samantha L Dyroff, James Derek Stensby, Andrea Evenski, Lester J Layfield
Skeletal Radiology.2021; 50(8): 1585. CrossRef - Hot shoulder PET/CT lesion: Unusual presentation of tenosynovial giant cell tumor
Steven Lewis, Lance Edmonds, Ely Wolin
Radiology Case Reports.2018; 13(3): 559. CrossRef
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Original Article
EGFR Gene Amplification and Protein Expression in Invasive Ductal Carcinoma of the Breast- Won Hwangbo, Jeong Hyeon Lee, Sangjeong Ahn, Seojin Kim, Kyong Hwa Park, Chul Hwan Kim, Insun Kim
- Korean J Pathol. 2013;47(2):107-115. Published online April 24, 2013
- DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.2.107
- 11,667 View
- 81 Download
- 14 Crossref
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Abstract
PDF
Background The epidermal growth factor receptor (EGFR) is a surrogate marker for basal-like breast cancer. A recent study suggested that EGFR may be used as a target for breast cancer treatment.
Methods A total of 706 invasive ductal carcinomas (IDC) of the breast were immunophenotyped, and 82 cases with EGFR protein expression were studied for
EGFR gene amplification.Results EGFR protein was expressed in 121 of 706 IDCs (17.1%); 5.9% were of luminal type, 25.3% of epidermal growth factor receptor 2 (HER-2) type, and 79.3% of basal-like tumors.
EGFR gene amplification and high polysomy (fluorescentin situ hybridization [FISH]-positive) were found in 18 of 82 cases (22.0%); 41.2% of the HER-2+, EGFR+, cytokeratin 5/6- (CK5/6-) group, 11.2% of the HER-2-, EGFR+, CK5/6- group, and 19.1% of the HER-2-, EGFR+, CK5/6+ group. FISH-positive cases were detected in 8.3% of the EGFR protein 1+ expression cases, 15.9% of 2+ expression cases, and 38.5% of 3+ expression cases. In group 2, the tumors had a high Ki-67 labeling (>60%), but the patients showed better disease-free survival than those with tumors that co-expressed HER-2 or CK5/6.Conclusions EGFR-directed therapy can be considered in breast cancer patients with EGFR protein overexpression and gene amplification, and its therapeutic implication should be determined in HER-2 type breast cancer patients.
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Citations
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