Department of Pathology, Korea University Anam Hospital, Seoul, Korea
© 2016 The Korean Society of Pathologists/The Korean Society for Cytopathology
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Positive | Negative | Total | |
---|---|---|---|
PCR | 101 (17.6) | 473 (82.4) | 574 (100) |
AFB stain | 8 (3.3) | 225 (96.7) | 233 (100) |
IGRA | 77 (55.4) | 62 (44.6) | 139 (100) |
Mycobacterial culture | 90 (22.2) | 315 (77.8) | 405 (100) |
Reference | Positive criteria | PCR methods | No. of cases | Type of specimens | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) |
---|---|---|---|---|---|---|---|---|
Park et al. [14] | Culture | Nested | 152 | FFPE, lung | 85 | 99 | 98 | 88 |
Frevel et al. [15] | Histology, culture, clinical finding | NA | 229 | FFPE, various organs | 90 | 92 | 81 | 96 |
Mishra et al. [16] | Culture | Real time | 28 | FFPE, gastrointestinal specimens | 96.4 | 100 | 100 | 96 |
Seo et al. [17] | Culture | Nested | 88 | FFPE, various organs | 87.5 | 98.2 | 96.6 | 93.2 |
Diaz et al. [18] | Clinical and microbiological | Nested | 43 | FFPE, liver | 58 | 96 | NA | NA |
Salian et al. [19] | Culture | NA | 60 | FFPE, various organs | 100 | 93 | 76.9 | 100 |
Lee et al. [20] | Culture | Nested | 129 | FFPE, various organ | 68.3 | 98.5 | NA | NA |
Present study | Culture | Nested | 405 | FFPE, various organ | 51.1 | 86.3 | 51.7 | 86.1 |
Clinicopathological characteristic | No. (%) |
---|---|
Sex | |
Male | 293 (51.0) |
Female | 281 (49.0) |
The way obtaining tissue | |
Surgical resection | 239 (41.6) |
Biopsy | 335 (58.4) |
Histology | |
Necrotizing granulomatous inflammation | 158 (27.5) |
Granulomatous inflammation without necrosis | 95 (16.6) |
Necrosis without granuloma | 45 (7.8) |
No granuloma or necrosis | 276 (48.1) |
Site | |
Gastrointestinaltract | 198 (34.5) |
Bone and soft tissue | 108 (18.8) |
Lymph node | 86 (15.0) |
Lung and pleura | 102 (17.8) |
Skin | 31 (5.4) |
Peritoneum | 18 (3.1) |
Head and neck | 14 (2.4) |
Brain | 3 (0.5) |
Adnexa, breast, pericardium, spleen, testis | Each 2 (0.3) |
Liver, kidney, bladder, thymus | Each 1 (0.17) |
Positive | Negative | Total | |
---|---|---|---|
PCR | 101 (17.6) | 473 (82.4) | 574 (100) |
AFB stain | 8 (3.3) | 225 (96.7) | 233 (100) |
IGRA | 77 (55.4) | 62 (44.6) | 139 (100) |
Mycobacterial culture | 90 (22.2) | 315 (77.8) | 405 (100) |
Method | Culture |
No. of cases | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | ||
---|---|---|---|---|---|---|---|---|
(+) | (–) | |||||||
PCR | (+) | 46 | 43 | 405 | 51.1 | 86.3 | 51.7 | 86.1 |
(–) | 44 | 272 | ||||||
AFB stain | (+) | 3 | 1 | 91 | 12.5 | 98.5 | 75.0 | 75.9 |
(–) | 21 | 66 | ||||||
IGRA | (+) | 21 | 30 | 91 | 80.8 | 53.8 | 41.2 | 87.5 |
(–) | 5 | 35 |
(+) | PCR | Culture |
Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |
---|---|---|---|---|---|---|---|
(+) | (–) | ||||||
Necrotizing granuloma (n = 131) | (+) | 36 | 32 | 65.5 | 57.9 | 52.9 | 69.8 |
(–) | 19 | 44 | |||||
Non-necrotizing granuloma (n = 61) | (+) | 5 | 8 | 26.3 | 81.0 | 38.5 | 70.8 |
(–) | 14 | 34 | |||||
Necrosis only (n = 38) | (+) | 2 | 3 | 66.7 | 91.4 | 40.0 | 97.0 |
(–) | 1 | 32 | |||||
No granuloma and necrosis (n = 175) | (+) | 3 | 2 | 23.1 | 98.8 | 60.0 | 94.2 |
(–) | 10 | 162 |
Method | к coefficient | p-value |
---|---|---|
PCR and culture | 0.376 | <.001 |
PCR and IGRA | 0.126 | .034 |
PCR and AFB stain | 0.235 | <.001 |
PCR and histopathologic features | 0.442 | <.001 |
Culture and positive result for both PCR and histopathologic features | 0.288 | <.001 |
Culture and positive result for PCR or histopathologic features | 0.388 | <.001 |
Reference | Positive criteria | PCR methods | No. of cases | Type of specimens | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) |
---|---|---|---|---|---|---|---|---|
Park et al. [14] | Culture | Nested | 152 | FFPE, lung | 85 | 99 | 98 | 88 |
Frevel et al. [15] | Histology, culture, clinical finding | NA | 229 | FFPE, various organs | 90 | 92 | 81 | 96 |
Mishra et al. [16] | Culture | Real time | 28 | FFPE, gastrointestinal specimens | 96.4 | 100 | 100 | 96 |
Seo et al. [17] | Culture | Nested | 88 | FFPE, various organs | 87.5 | 98.2 | 96.6 | 93.2 |
Diaz et al. [18] | Clinical and microbiological | Nested | 43 | FFPE, liver | 58 | 96 | NA | NA |
Salian et al. [19] | Culture | NA | 60 | FFPE, various organs | 100 | 93 | 76.9 | 100 |
Lee et al. [20] | Culture | Nested | 129 | FFPE, various organ | 68.3 | 98.5 | NA | NA |
Present study | Culture | Nested | 405 | FFPE, various organ | 51.1 | 86.3 | 51.7 | 86.1 |
Values are presented as number (%). PCR, polymerase chain reaction; AFB, acid-fast bacilli; IGRA, tuberculosis-specific antigen-induced interferon gamma release assay.
PPV, positive predictive value; NPV, negative predictive value; PCR, polymerase chain reaction; AFB, acid-fast bacilli; IGRA, tuberculosis-specific antigen-induced interferon gamma release assay.
PCR, polymerase chain reaction; PPV, positive predictive value; NPV, negative predictive value.
PCR, polymerase chain reaction; IGRA, tuberculosis-specific antigen-induced interferon gamma release assay; AFB, acid-fast bacilli.
PCR, polymerase chain reaction; PPV, positive predictive value; NPV, negative predictive value; FFPE, formalin fixed, paraffin embedded tissue; NA, not available.