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Original Articles
- The proteomic landscape shows oncologic relevance in cystitis glandularis
- Jun Yong Kim, Dohyun Han, Hyeyoon Kim, Minsun Jung, Han Suk Ryu
- J Pathol Transl Med. 2023;57(1):67-74. Published online December 22, 2022
- DOI: https://doi.org/10.4132/jptm.2022.10.24
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Abstract
PDF - Background
The relationship between cystitis glandularis (CG) and bladder malignancy remains unclear.
Methods
We identified the oncologic significance of CG at the molecular level using liquid chromatography-tandem mass spectrometry-based proteomic analysis of 10 CG, 12 urothelial carcinoma (UC), and nine normal urothelium (NU) specimens. Differentially expressed proteins (DEPs) were identified based on an analysis of variance false discovery rate < 0.05, and their functional enrichment was analyzed using a network model, Gene Set Enrichment Analysis, and Gene Ontology annotation.
Results
We identified 9,890 proteins across all samples and 1,139 DEPs among the three entities. A substantial number of DEPs overlapped in CG/NU, distinct from UC. Interestingly, we found that a subset of DEP clusters (n = 53, 5%) was differentially expressed in NU but similarly between CG and UC. This “UC-like signature” was enriched for reactive oxygen species (ROS) and energy metabolism, growth and DNA repair, transport, motility, epithelial-mesenchymal transition, and cell survival. Using the top 10 shortlisted DEPs, including SOD2, PRKCD, CYCS, and HCLS1, we identified functional elements related to ROS metabolism, development, and transport using network analysis. The abundance of these four molecules in UC/CG than in NU was consistent with the oncologic functions in CG.
Conclusions
Using a proteomic approach, we identified a predominantly non-neoplastic landscape of CG, which was closer to NU than to UC. We also confirmed a small subset of common DEPs in UC and CG, suggesting that altered ROS metabolism might imply potential cancerous risks in CG.
- Evaluation of Urine Cytology in Urothelial Carcinoma Patients: A Comparison of CellprepPlus® Liquid-Based Cytology and Conventional Smear
- Seung-Myoung Son, Ji Hae Koo, Song-Yi Choi, Ho-Chang Lee, Yong-Moon Lee, Hyung Geun Song, Hae-Kyung Hwang, Hye-Suk Han, Seok-Joong Yun, Wun-Jae Kim, Eun-Joong Kim, Ok-Jun Lee
- Korean J Pathol. 2012;46(1):68-74. Published online February 23, 2012
- DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.1.68
- 9,455 View
- 79 Download
- 10 Crossref
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Abstract
PDF
Background Urine cytology is an important test in the screening of urothlelial neoplasms. The conventional smear (CS) method of testing urine samples has a low sensitivity, approximately 50% result accuracy for detecting urothelial carcinomas, while liquid-based cytology (LBC) has much improved diagnostic accuracy, sensitivity, and specificity. The aim of this study was to compare the morphologic features and diagnostic efficacy of CellprepPlus® LBC with those of CS for urine cytology.
Methods A total of 713 cases of urine specimens collected from November 2009 to September 2010 were included. All specimens were divided equally for the preparation of CellprepPlus® LBC and CS for each case.
Results CellprepPlus® revealed more cellularity, a cleaner background and better cytomorphologic features, but it showed a less intact architectural pattern compared to that of CS. Of the 88 histologically confirmed cases, the diagnostic sensitivity for CellprepPlus® was 50% and higher than the 37.5% for CS. The specificity of both preparations was 100%.
Conclusions The CellprepPlus® showed an improved quality of slides and provided better diagnostic accuracy, thus CellprepPlus® could be a first-line screening tool in urinary tract cytology.
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Citations
Citations to this article as recorded by
- Deep Learning-Based Screening of Urothelial Carcinoma in Whole Slide Images of Liquid-Based Cytology Urine Specimens
Masayuki Tsuneki, Makoto Abe, Fahdi Kanavati
Cancers.2022; 15(1): 226. CrossRef - Diagnostic efficacy of smear plus liquid-based cytology for EUS-FNA of solid pancreatic lesions
Masahiro Itonaga, Shin-Ichi Murata, Keiichi Hatamaru, Takashi Tamura, Junya Nuta, Yuki Kawaji, Takao Maekita, Mikitaka Iguchi, Jun Kato, Fumiyoshi Kojima, Hiroki Yamaue, Manabu Kawai, Ken-Ichi Okada, Seiko Hirono, Toshio Shimokawa, Kensuke Tanioka, Masayu
Medicine.2019; 98(19): e15575. CrossRef - Evaluation and application of Cellprep for cervical cytology
Rinko OZEKI, Keiichi IWAYA, Yuko UMAYAHARA, Yuka MORITA, Mie ARAI, Yukari TAKASUGI, Ryoko KIKUCHI, Kiyohiko MIYAKE, Atsuhiko SAKAMOTO, Masaru SAKAMOTO
The Journal of the Japanese Society of Clinical Cytology.2018; 57(3): 159. CrossRef - Quantitative Proteomic Analysis Identifies AHNAK (Neuroblast Differentiation-associated Protein AHNAK) as a Novel Candidate Biomarker for Bladder Urothelial Carcinoma Diagnosis by Liquid-based Cytology
Hyebin Lee, Kwangsoo Kim, Jongmin Woo, Joonho Park, Hyeyoon Kim, Kyung Eun Lee, Hyeyeon Kim, Youngsoo Kim, Kyung Chul Moon, Ji Young Kim, In Ae Park, Bo Bae Shim, Ji Hye Moon, Dohyun Han, Han Suk Ryu
Molecular & Cellular Proteomics.2018; 17(9): 1788. CrossRef - Reliability of Estrogen Receptor and Human Epidermal Growth Factor Receptor 2 Expression on Breast Cancer Cells Stored in Cellprep® Vials
Ayumi Ryu, Jyun-ichi Ashimura, Takahiro Nakayama, Yasuhiro Tamaki, Shin-ichi Nakatsuka, Yasuhiko Tomita
Acta Cytologica.2018; 62(5-6): 360. CrossRef - Morphologic Analysis of Cytomegalovirus Infected Cells in Bronchial Washing Cytology: Comparison of Liquid-Based Preparation and Conventional Smear
Jae Yeon Seok, Jungsuk An, Seung Yeon Ha, Dong Hae Chung, Sangho Lee, Hyunchul Kim
Journal of Pathology and Translational Medicine.2016; 50(2): 147. CrossRef - Romanowsky staining using liquid‐based cytology: A pilot study using Cytolyt®/HESPANDER® processing solution for ThinPrep® preparations
Yuichi Kinoshita, Takashi Yuri, Katsuhiko Yoshizawa, Kosho Takasu, Yuko Emoto, Airo Tsubura, Nobuaki Shikata
Diagnostic Cytopathology.2015; 43(12): 960. CrossRef - Diagnostic Value of Liquid-Based Cytology in Urothelial Carcinoma Diagnosis: A Systematic Review and Meta-Analysis
You Luo, Dong-Li She, Hu Xiong, Li Yang, Sheng-Jun Fu, Francisco X. Real
PLOS ONE.2015; 10(8): e0134940. CrossRef - Comparison of diagnostic accuracy between CellprepPlus® and ThinPrep® liquid‐based preparations in effusion cytology
Yong‐Moon Lee, Ji‐Yong Hwang, Seung‐Myoung Son, Song‐Yi Choi, Ho‐Chang Lee, Eun‐Joong Kim, Hye‐Suk Han, Jin young An, Joung‐Ho Han, Ok‐Jun Lee
Diagnostic Cytopathology.2014; 42(5): 384. CrossRef - Diagnostic Efficacy of Cell Block Immunohistochemistry, Smear Cytology, and Liquid-Based Cytology in Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Lesions: A Single-Institution Experience
Shan-yu Qin, You Zhou, Ping Li, Hai-xing Jiang, Robert L. Schmidt
PLoS ONE.2014; 9(9): e108762. CrossRef
- Deep Learning-Based Screening of Urothelial Carcinoma in Whole Slide Images of Liquid-Based Cytology Urine Specimens
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