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The Korean Journal of Pathology 1978;12(3): 219-224.
Platelet factor 3 assay를 이용한 혈소판 감소증 및 임부 혈청의 혈소판 항체의 검색
Detection of Platelet Antibody in the sera of Thrombocytopenia Subject and Gravida by Platelet factor 3 Assay.
ABSTRACT
The increasing use of platelet transfusion in the treatment of thrombocytopenia has stimulated significant advances in the understanding of platelet immunology. A number of antigens which are also shared by granulocytes and Iymphocytes have been found on platelets. These have been shown more recently to be histocompatibility antigens, which are clinically important in causing neonatal and posttransfusion thrombocytopenia and in decreasing the platelet survival of transfused platelets in individuals receiving multiple transfusions. In recent years, many attempts have been made to replace the cumbersome and insensitive complement fixation test with more sensitive methods. These tests have included inhibition of serotonine uptake, release of 51Cr from platelets and assay of platelet factor 3. In this study, the simplified platelet factor 3 assay (Hirschman & Gralnick, 1974) was evaluated in 50 sera from thrombocytopenia with various clinical disorders and in 20 sera from the pregnant women. The results are as follows; 1. About 40% of patients with thrombocytopenia had the malignant tumors and leukemia. Other clinical disorders with thrombocytopenia include hepatic disease, idiopathic thrombocytopenic purpura, disseminated intravascular coagulation, salmonellosis, aplastic anemia and etc. 2. Detection of platelet antibody by PF 3 assay were significantly frequent in the patients with past history of transfusions. 3. By PF 3 assay, platelet antibodies are detectable in 9 cases among 20 pregnant women and the positive frequency was increased with the number of pregnancy. 4. By PF 3 assay, platelet antibody was detectable in 3 out of 4 cases of idiopathic thrombocytopenic purpura. 5. The platelet factor 3 assay appear to be sensitive and applicable in most clinical laboratories. 6. The PF 3 assay may be used as a pretransfusion compatibility test for the patients refractory to platelet transfusions. 7. Studies on the false positive reactions and reproducibility need to be further investigated.
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