Surveys for laboratory methods of 20 chemical test items were performed in 1978 and 1979. 57 and 34 laboratories of general hospitals participated in 1978 and 1979 respectively. The most popular methods were as follows; Items Method 1978 1979 Total Bilirubin Evelyn-Malloy meth. 74.5(%) 75.8(%) Cholesterol Lieberman-Burchard meth 87.2 72.7 Triglyceride Enzyme-Colorimetry 56.3 78.9 Glucose Orthotoluidine meth 52.0 47.1 Calcium Cresolphthlein complexone meth 71.5 83.4 Phosphorus Fiske-Subbarow meth. 75.7 51.7 Sodium Flame photometry 100.0 100.0 Potassium Flame photometry 100.0 100.0 Chloride Schales-Schales meth. 93.0 81.3 Protein Biuret meth. 94.1 91.2 Albumin Bromcresol green meth. 78.3 58.8 Protein Electrophoresis Cellulose Acetate meth. 89.5 Urea Nitrogen Urease Indophenol 56.9 64.7 Uric acid Phosphotungstate meth. 89.5 96.8 Creatinine Jaffe reaction meth. 100.0 100.0 SGOT Reitman-Frankel meth. 100.0 100.0 SGPT Reitman-Frankel meth. 100.0 100.0 LDH (P-L) NADH Colorimetry 96.8 82.6 AlK. Phosphatase Bessey-Lowry Brock meth. 40.4 52.9 Kind-King meth. 48.1 29.4 Amylase Amyloclastic meth. 100.0 88.2