Analysis of HPV-other Samples by Performing HPV DNA Sequencing.

Yoo Duk Choi; Chang Woo Han; Woon Jae Chung; Woon Won Jung; Ji Shin Lee; Jong Hee Nam; Min Cheol Lee; Sang Woo Juhng; Ho Sun Choi; Chang Soo Park

doi:10.4132/KoreanJPathol.2009.43.3.250

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Original Article Analysis of HPV-other Samples by Performing HPV DNA Sequencing.: Yoo Duk Choi, Chang Woo Han, Woon Jae Chung, Woon Won Jung, Ji Shin Lee, Jong Hee Nam, Min Cheol Lee, Sang Woo Juhng, Ho Sun Choi, Chang Soo Park; Journal of Pathology and Translational Medicine 2009;43(3):250-253
DOI: https://doi.org/10.4132/KoreanJPathol.2009.43.3.250

¹Department of Pathology, Chonnam National University Medical School, Gwangju, Korea. daniel5438@hanmail.net
²MyGene Bioscience institute, Mygene Company, Seoul, Korea.
³Department of Obstetric and Gynecology, Chonnam National University Medical School, Gwangju, Korea.
⁴Brain Korea 21 Project, Center for Biomedical Human Resources at Chonnam National University, Gwangju, Korea.

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Abstract

BACKGROUND
HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.
METHODS
For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high-risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV-other samples of 209 patients who subsequently underwent cervical biopsy.
RESULTS
For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.
Conclusion
HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.

Keywords: DNA chips;Papillomaviridae;Sequence analysis, Uterine cervix

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