Fig. 1Necroinflammatory activity and fibrosis of adjacent peritumoral non-neoplastic liver parenchyma (PNLP) showing (A) division of PNLP with distance from tumor border from the left to right side (scanning view), (B-E) representative pictures of spotty necrosis in each 5-mm column (B, 5-mm; C, 10-mm; D, 15-mm; E, 20-mm), (F) fibrosis of PNLP and segmentation blocks for image analysis of collagen deposition (Masson's trichrome [M-T] stain, scanning view).
Fig. 2Necroinflammatory activity of adjacent peritumoral non-neoplastic liver parenchymas howing (A, B) portal inflammation, (C, D) periportal inflammation, (E, F) spotty necrosis (all based on semiquantitative four grade system), and (G, H) the modified histologic activity index (HAI) grading. The center of the circle represents the tumor border, and each concentric circle line indicates an increment of 5 mm from the tumor. C1-C20 indicate each case (C1=case 1). ap-value of Wilcoxon signed-rank test between two adjacent columns <0.05.
Fig. 3Bile ductular proliferation, peliosis-like lesions, and heptic venulitis in (A) 5-mm column, (B) 15-mm column, (C) 5-mm column, (D) 5-mm column.
Fig. 4Peliosis and hepatic venulitis. (A, B) The number of peliosis-like lesions per 4× objective field. (C, D) Hepatic venulitis. ap-value of Wilcoxon signed-rank test between two adjacent columns <0.05.
Fig. 5Architectural changes including fibrosis and bile ductular proliferation. (A) Ishak fibrosis staging system. (B) Collagen deposition by image analysis. (C, D) Bile ductular proliferation. ap-value of Wilcoxon signed-rank test between two adjacent columns <0.05.
Table 1The mean values of the pathologic parameters for each column and the p-values for the differences between columns