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- Evaluation of the VE1 Antibody in Thyroid Cytology Using Ex Vivo Papillary Thyroid Carcinoma Specimens
- Yon Hee Kim, Hyunee Yim, Yong-Hee Lee, Jae Ho Han, Kyi Beom Lee, Jeonghun Lee, Euy Young Soh, Seon-Yong Jeong, Jang-Hee Kim
- J Pathol Transl Med. 2016;50(1):58-66. Published online December 14, 2015
- DOI: https://doi.org/10.4132/jptm.2015.10.10
- 9,477 View
- 76 Download
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Abstract
PDF - Background
Recently, VE1, a monoclonal antibody against the BRAFV600E mutant protein, has been investigated in terms of its detection of the BRAFV600E mutation. Although VE1 immunostaining and molecular methods used to assess papillary thyroid carcinoma in surgical specimens are in good agreement, evaluation of VE1 in thyroid cytology samples is rarely performed, and its diagnostic value in cytology has not been well established. In present study, we explored VE1 immunoexpression in cytology samples from ex vivo papillary thyroid carcinoma specimens in order to minimize limitations of low cellularity and sampling/targeting errors originated from thyroid fineneedle aspiration and compared our results with those obtained using the corresponding papillary thyroid carcinoma tissues. Methods: The VE1 antibody was evaluated in 21 cases of thyroid cytology obtained directly from ex vivo thyroid specimens. VE1 immunostaining was performed using liquid-based cytology, and the results were compared with those obtained using the corresponding tissues. Results: Of 21 cases, 19 classic papillary thyroid carcinomas had BRAFV600E mutations, whereas two follicular variants expressed wild-type BRAF. VE1 immunoexpression varied according to specimen type. In detection of the BRAFV600E mutation, VE1 immunostaining of the surgical specimen exhibited 100% sensitivity and 100% specificity, whereas VE1 immunostaining of the cytology specimen exhibited only 94.7% sensitivity and 0% specificity. Conclusions: Our data suggest that VE1 immunostaining of a cytology specimen is less specific than that of a surgical specimen for detection of the BRAFV600E mutation, and that VE1 immunostaining of a cytology specimen should be further evaluated and optimized for clinical use. -
Citations
Citations to this article as recorded by
- Principles of Analytic Validation of Immunohistochemical Assays: Guideline Update
Jeffrey D. Goldsmith, Megan L. Troxell, Sinchita Roy-Chowdhuri, Carol F. Colasacco, Mary Elizabeth Edgerton, Patrick L. Fitzgibbons, Regan Fulton, Thomas Haas, Patricia L. Kandalaft, Tanja Kalicanin, Christina Lacchetti, Patti Loykasek, Nicole E. Thomas,
Archives of Pathology & Laboratory Medicine.2024; 148(6): e111. CrossRef - Inter- and Intra-observer Reproducibility of Thyroid Fine Needle Aspiration Cytology: An investigation of Bethesda 2023 Using Immunohistochemical BRAFV600E Antibody
Erdogan Bahattin, Dündar Emine, Çetin Kısmet Çivi, Yılmaz Fatih
Journal of Cytology.2024; 41(4): 221. CrossRef - VE1 immunohistochemistry is an adjunct tool for detection of BRAFV600E mutation: Validation in thyroid cancer patients
Faiza A. Rashid, Sobia Tabassum, Mosin S. Khan, Hifzur R. Ansari, Muhammad Asif, Ahmareen K. Sheikh, Syed Sameer Aga
Journal of Clinical Laboratory Analysis.2021;[Epub] CrossRef - Effective utilization of liquid-based cytology for thyroid lesions
Yukie YAMAYA
The Journal of the Japanese Society of Clinical Cytology.2021; 60(3): 164. CrossRef - Diagnostic Efficacy of BRAFV600E Immunocytochemistry in Thyroid Aspirates in Bethesda Category IV and Papillary Thyroid Carcinoma
Nidhi Anand, Tushar Agrawal, Anurag Gupta, Saumya Shukla, Roma Pradhan, Nuzhat Husain
Journal of Cytology.2021; 38(3): 113. CrossRef - The immunocytochemical expression of VE‐1 (BRAF V600E‐related) antibody identifies the aggressive variants of papillary thyroid carcinoma on liquid‐based cytology
Patrizia Straccia, Chiara Brunelli, Esther D. Rossi, Paola Lanza, Maurizio Martini, Teresa Musarra, Celestino Pio Lombardi, Alfredo Pontecorvi, Guido Fadda
Cytopathology.2019; 30(5): 460. CrossRef - Utility of the BRAF p.V600E immunoperoxidase stain in FNA direct smears and cell block preparations from patients with thyroid carcinoma
Amber L. Smith, Michelle D. Williams, John Stewart, Wei‐Lien Wang, Savitri Krishnamurthy, Maria E. Cabanillas, Sinchita Roy‐Chowdhuri
Cancer Cytopathology.2018; 126(6): 406. CrossRef - Refinement of the criteria for ultrastructural peritubular capillary basement membrane multilayering in the diagnosis of chronic active/acute antibody-mediated rejection
Heounjeong Go, Sung Shin, Young Hoon Kim, Duck Jong Han, Yong Mee Cho
Transplant International.2017; 30(4): 398. CrossRef - Thyroid Fine-Needle Aspiration Cytology Practice in Korea
Yoon Jin Cha, Ju Yeon Pyo, SoonWon Hong, Jae Yeon Seok, Kyung-Ju Kim, Jee-Young Han, Jeong Mo Bae, Hyeong Ju Kwon, Yeejeong Kim, Kyueng-Whan Min, Soonae Oak, Sunhee Chang
Journal of Pathology and Translational Medicine.2017; 51(6): 521. CrossRef - Use of monoclonal antibodies to detect specific mutations in formalin-fixed, paraffin-embedded tissue sections
Zhenying Guo, Ricardo V. Lloyd
Human Pathology.2016; 53: 168. CrossRef
- Principles of Analytic Validation of Immunohistochemical Assays: Guideline Update
- Renal Histologic Parameters Influencing Postoperative Renal Function in Renal Cell Carcinoma Patients
- Myoung Ju Koh, Beom Jin Lim, Kyu Hun Choi, Yon Hee Kim, Hyeon Joo Jeong
- Korean J Pathol. 2013;47(6):557-562. Published online December 24, 2013
- DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.6.557
- 5,855 View
- 44 Download
- 4 Crossref
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Abstract
PDF
Background Pre-existing non-neoplastic renal diseases or lesions may influence patient renal function after tumor removal. However, its description is often neglected or omitted in pathologic reports. To determine the incidence and clinical significance of non-neoplastic lesions, we retrospectively examined renal tissues obtained during 85 radical nephrectomies for renal cell carcinoma.
Methods One paraffin-embedded tissue block from each case containing a sufficient amount of non-tumorous renal parenchyma was cut and processed with hematoxylin and eosin and periodic acid-Schiff methods. Non-neoplastic lesions of each histological compartment were semi-quantitatively and quantitatively evaluated.
Results Among the various histologic lesions found, tubular atrophy, arterial intimal thickening, and glomerulosclerosis were the most common (94.1%, 91.8%, and 88.2%, respectively). Glomerulosclerosis correlated with estimated glomerular filtration rate at the time of surgery, as well as at 1- and 5-years post-surgery (p=.0071), but tubulointerstitial fibrosis or arterial fibrous intimal thickening did not.
Post-hoc analysis revealed that glomerulosclerosis of more than 20% predicted post-operative renal function. However, its significance disappeared when gender and age were considered.Conclusions In conclusion, non-neoplastic lesions, especially with regard to glomerulosclerosis percentage, should be described in pathology reports to provide additional information on renal function decline.
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Citations
Citations to this article as recorded by- Role of intravoxel incoherent motion diffusion-weighted MRI in differentiation of renal cell carcinoma subtypes
Amira R. Mahmoud, Nehad Fouda, Eman Mohamed Helmy, Ali Elsorougy
Egyptian Journal of Radiology and Nuclear Medicine.2024;[Epub] CrossRef - Chronic kidney damage pathology score for systematic assessment of the non-neoplastic kidney tissue and prediction of post-operative renal function outcomes
Yong Jia, Seyed M.M. Poor, Brenden Dufault, Vivian Lu, Jasmir G. Nayak, Deepak K. Pruthi, Ian W. Gibson
Human Pathology.2022; 124: 76. CrossRef - Value of intravoxel incoherent motion for differential diagnosis of renal tumors
Qingqiang Zhu, Wenrong Zhu, Jing Ye, Jingtao Wu, Wenxin Chen, Zhihua Hao
Acta Radiologica.2019; 60(3): 382. CrossRef - Conventional and Papillary Renal Cell Carcinomas and Focal Segmental Glomerulosclerosis in a Nephrectomy
Firas Al-Delfi, Guillermo A. Herrera
Pathology Case Reviews.2015; 20(6): 263. CrossRef
- Role of intravoxel incoherent motion diffusion-weighted MRI in differentiation of renal cell carcinoma subtypes
- Urinary Decoy Cell Grading and Its Clinical Implications
- Myoung Ju Koh, Beom Jin Lim, Songmi Noh, Yon Hee Kim, Hyeon Joo Jeong
- Korean J Pathol. 2012;46(3):233-236. Published online June 22, 2012
- DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.3.233
- 8,363 View
- 60 Download
- 6 Crossref
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Abstract
PDF
Background Examination of urine for decoy cells (DCs) is a useful screening test for polyomavirus (PV) activation. We explored the significance of the amount of DCs in persistent shedding, PV nephropathy and acute rejection.
Methods A case-controlled study was performed in 88 renal allograft patients who had DCs detected at least once in four or more urine samples.
Results Fifty one patients were classified into the high-grade shedding group (HG) and 37 patients into the low-grade shedding group (LG) according to DC shedding (≥10 or <10 DCs/10 high power field [HPF]). DC shedding of more than three consecutive months was significantly more prevalent in the HG as compared with their LG counterparts (p<0.0001). Urinary DCs were present for more than one year in 29.4% of the HG and 8.1% of the LG. Real-time polymerase chain reaction for PV was higher in both urine (51.4% vs. 11.1%) and plasma (9.1% vs. 0%) of the HG than the LG. The prevalence of PV nephropathy was higher in the HG than the LG (p=0.019). However, there was no significant difference in the prevalence of acute rejection.
Conclusions Shedding of ≥10 DCs/10 HPF is associated with sustained shedding, polymerase chain reaction positivity and PV nephropathy, but not a predictor of acute rejection.
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Citations
Citations to this article as recorded by- Polyomavirus nephropathy: diagnosis, histologic features, and differentiation from acute rejection
Cynthia C. Nast
Clinical Transplantation and Research.2024; 38(2): 71. CrossRef - Challenges and opportunities in research on BK virus infection after renal transplantation
Yukun Tang, Zipei Wang, Dunfeng Du
International Immunopharmacology.2024; 141: 112793. CrossRef - BK Virus-Associated Nephropathy after Renal Transplantation
Yasuhito Funahashi
Pathogens.2021; 10(2): 150. CrossRef - Diagnostic utility of urine cytology in detection of decoy cells in renal transplant patients: Report of five cases and review of literature
Santosh Tummidi, Kanchan Kothari, Mona Agnihotri, Leena Naik, Amey Rojekar
Diagnostic Cytopathology.2020; 48(3): 222. CrossRef - Association of Pretransplant BK Polyomavirus Antibody Status with BK Polyomavirus Infection After Kidney Transplantation: A Prospective Cohort Pilot Study of 47 Transplant Recipients
Yu Hisadome, Hiroshi Noguchi, Yuki Nakafusa, Kukiko Sakihama, Takanori Mei, Keizo Kaku, Yasuhiro Okabe, Kosuke Masutani, Yuki Ohara, Kazuyuki Ikeda, Yoshinao Oda, Masafumi Nakamura
Transplantation Proceedings.2020; 52(6): 1762. CrossRef - Association Between the Polyomaviruses Titers and Decoy Cell Positivity Rates After Renal Transplantation
Y. Funahashi, M. Kato, T. Fujita, S. Ishida, A. Mori, M. Gotoh
Transplantation Proceedings.2016; 48(3): 921. CrossRef
- Polyomavirus nephropathy: diagnosis, histologic features, and differentiation from acute rejection
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