ATRX |
Nucleus |
Loss of function mutation |
Negative |
Diffuse and anaplastic astrocytoma |
β-Catenin |
Nucleus |
Gain of function mutation |
Focal positive |
WNT type medulloblastoma, adamantinomatous craniopharyngioma |
BRAF VE1 (BRAF V600E) |
Cytoplasm |
Gain of function mutation |
Positive |
Pleomorphic xanthoastrocytoma, ganglioglioma, pilocytic astrocytoma, epithelioid glioblastoma, papillary craniopharyngioma |
BRG1 |
Nucleus |
Gain of function mutation |
Negative |
Atypical teratoid rhabdoid tumor |
CIC |
Nucleus |
Loss of function mutation |
Negative |
Oligodendroglioma (47%) [15] |
c-MET |
Membrane |
Overexpression |
Positive |
Glioblastoma, anaplastic astrocytoma |
EGFR |
Membrane |
Overexpression |
Positive |
Glioblastoma, anaplastic astrocytoma |
EGFRvIII |
Membrane |
Overexpression |
Positive |
Glioblastoma, anaplastic astrocytoma |
H3 K27M |
Nucleus |
Gain of function mutation |
Positive |
Diffuse midline glioma |
FUBP1 |
Nucleus |
Loss of function mutation |
Negative |
Oligodendroglioma (16%) [15] |
IDH1 (H09) |
Nucleus and cytoplasm |
Gain of function mutation |
Positive |
Astrocytoma and oligodendroglioma |
INI1 |
Nucleus |
Loss of function mutation |
Negative |
Atypical teratoid rhabdoid tumor |
P16 |
Nucleus and cytoplasm |
Loss of function mutation |
Negative |
High grade glioma |
P53 |
Nucleus |
Overexpression |
Positive |
Astrocytic tumors |
PDGFRA |
Membrane |
Overexpression |
Positive |
Glioblastoma, anaplastic astrocytoma |
PTEN |
Cytoplasm |
Loss of function mutation |
Negative |
Glioblastoma |
RELA (NFKB3) |
Cytoplasm |
Gain of function mutation |
Positive |
Cerebral ependymoma |
STAT6 |
Nucleus |
Gain of function mutation |
Positive |
Solitary fibrous tumor/hemangiopericytoma |
MLH1 |
Nucleus |
Loss of function mutation |
Negative |
Gliomas |
MSH2 |
Nucleus |
Loss of function mutation |
Negative |
Gliomas |
PMS2 |
Nucleus |
Loss of function mutation |
Negative |
Gliomas |
Ki67 |
Nucleus |
Overexpression |
Positive |
Brain tumors (for an ancillary test for tumor grading) |
pHH3 |
Nucleus |
Overexpression |
Positive |
Brain tumors (for counting mitoses) |
GFAP |
Nucleus |
Expression |
Positive |
Astrocytic tumors |
Olig2 |
Nucleus |
Expression |
Positive |
Gliomas including astrocytomas and oligodendroglioma |
|
|
|
|
Neither neuronal nor ependymal tumors |