Gallbladder cancer |
174 |
FFPE tissue; anti–PD-L1 (clone SP263) |
1, 10, 50 |
Significant positive association with histologic type (squamous cell carcinoma, adenosquamous cell carcinoma, undifferentiated carcinoma), histologic grade (progressed from WD to PD), nuclear grade, stage 3 and 4, TIL (0 to 3+) |
OS was not associated with PD-L1 expression |
Neyaz et al. [11] |
Gallbladder adenocarcinoma |
66 |
FFPE tissue; anti–PD-L1 (E1L3N) |
5 |
PD-L1 positive alone was not correlated with any clinicopathological or pathological parameters except for CD8+ TIL density and worse median OS |
Combination of CD8 high with negative expression of PD-L1 serves as prognostic factor for improved OS and PFS |
Lin et al. [12] |
Gastric adenocarcinoma |
240 |
FFPE tissue; anti–PD-L1 (E1L3N) |
10 |
Patients with poor tumor differentiation had a higher positive rate of PD-L1 expression on tumor cells |
Positive PD-L1 expression on TILs had a shorter OS; However, PD-L1 expression on tumor cells was not associated with OS |
Fang et al. [23] |
Gastric cancer |
107 |
FFPE tissue; anti–PDL1 (polyclonal antihuman PD-L1/CD274 antibody) |
Not applicable |
Positive rate of PD-L1 expression is much higher in depth of invasion, high differentiation, lymph node metastasis, and higher T category |
PD-L1–positive gastric cancers were significantly associated with a poor prognosis |
Qing et al. [24] |
Esophageal cancer |
41 |
Frozen tissue; anti–PD-L1 (MIH1, mouse IgG1) |
10 |
Effect of PD-L1 status was more distinct in the advanced stage of tumor with lymph node metastasis and distinct metastasis |
Overall survival of patients with tumors positive for both PD-L1 and PD-L2 was significantly worse than that with tumors negative for both |
Ohigashi et al. [25] |
Colorectal cancer |
143 |
FFPE tissue; anti–PD-L1 (Abcam, ab58810) |
Strong and moderate immunostaining intensity |
PD-L1 was significantly associated with cell differentiation status and TNM stage |
Positive PD-L1 expression showed a trend shorter survival time; as an independent predictor of prognosis |
Shi et al. [26] |
Lung adenocarcinoma |
163 |
FFPE tissue; anti–PD-L1 (Proteintech Group Inc., Chicago, IL, USA) |
5 |
PD-L1 had higher positive results in tumors with higher grade differentiation and vascular invasion |
PD-L1 expression correlated with better RFS |
Yang et al. [27] |
Lung non-small cell carcinoma |
819 |
FFPE tissue; anti–PD-L1 (22C3) |
50 |
Lower PD-L1 positivity correlated with lower stage and squamous cell carcinoma than adenocarcinoma |
Not assessed |
Skov et al. [28] |
Extrahepatic cholangiocarcinoma |
69 |
FFPE tissue; anti–PD-L1 (E1L3N) |
Not applicable |
Significant correlations of PD-L1 expression with venous invasion and poor differentiation of the tumor were observed |
PD-L1 expression was not correlated with patient OS, but combined high PDL1 expression on tumor cells and low infiltration of CD3+ TILs showed poor OS |
Walter D et al. [29] |
Hepatocellular carcinoma |
240 + additional 125 |
FFPE tissue; anti–PD-L1 (eBioscience) |
High vs. low |
PD-L1 expression was an independent prognostic factor for tumor vascular invasion, encapsulation, and TNM stage |
PD-L1–positive (high expression) patients had significantly poorer DFS and OS |
Gao et al. [30] |
Hepatocellular carcinoma |
448 |
FFPE tissue; anti–PD-L1 (E1L3N) |
1, 5 |
No significant difference in PD-L1 expression was detected |
Survival analysis showed that 5% PD-L1 expression was significantly correlated with improved rates of OS and RFS |
Chen et al. [31] |
Uveal melanoma |
67 |
FFPE tissue; anti–PD-L1 (E1L3N) |
5 |
Significant association of PD-L1 expression to a decreased number of TIL |
PD-L1 expression is associated with metastasis-free survival |
Zoroquiain et al. [32] |