Fig. 1Microscopic images of immunohistochemical staining. Heat shock protein 70 (A-C), Ki-67 (D-F), and p53 (G-I). Left panels are from non-neoplastic gastric mucosa (A, D, G), middle panels are negative expression samples from gastric adenocarcinoma (B, E, H), and right panels are positive expression samples from gastric adenocarcinoma (C, F, I).
Fig. 2Analysis of gastric adenocarcinoma patient survival. Kaplan-Meier plots with heat shock protein 70 (HSP70) expression data in all cases of gastric adenocarcinoma including the early and advanced gastric carcinoma subgroups (A), early gastric carcinoma (B), and advanced gastric carcinoma (C). Lower graphs show Kaplan-Meier plots of Ki-67 expression in all gastric adenocarcinoma cases (D), early gastric carcinoma (E), and advanced gastric carcinoma (F). p-values obtained from log-rank tests are shown in each graph.
Table 1.Correlations between HSP70 expression and clinicopathologic characteristics
Clinicopathologic characteristic |
All gastric carcinoma (EGC and AGC)
|
EGC
|
AGC
|
No. of patients |
HSP70
|
No. of patients |
HSP70
|
No. of patients |
HSP70
|
Positive |
p-value |
Positive |
p-value |
Positive |
p-value |
Total |
|
458 |
155 (34) |
|
184 |
85 (46) |
|
274 |
70 (26) |
|
Sex |
Male |
311 |
113 (36) |
0.101 |
123 |
56 (46) |
0.797 |
188 |
57 (30) |
0.007 |
Female |
147 |
42 (29) |
|
61 |
29 (48) |
|
86 |
13 (15) |
|
Age (yr) |
< 60 |
214 |
79 (37) |
0.193 |
86 |
44 (51) |
0.206 |
128 |
35 (27) |
0.523 |
≥ 60 |
244 |
76 (31) |
|
98 |
41 (42) |
|
146 |
35 (24) |
|
Histologic grade |
WD |
74 |
26 (35) |
0.007 |
56 |
24 (43) |
0.538 |
18 |
2 (11) |
0.046 |
MD |
165 |
74 (45) |
|
83 |
39 (47) |
|
82 |
35 (43) |
|
PD |
219 |
55 (25) |
|
45 |
22 (49) |
|
174 |
33 (19) |
|
Lauren classification |
Intestinal |
239 |
100 (42) |
< 0.001 |
139 |
63 (45) |
0.677 |
100 |
37 (37) |
0.001 |
Diffuse |
219 |
55 (25) |
|
45 |
22 (49) |
|
174 |
33 (19) |
|
pT categorya
|
pT1 |
184 |
85 (46) |
< 0.001 |
184 |
85 (46) |
NA |
NA |
NA |
NA |
pT2 |
62 |
19 (31) |
|
NA |
NA |
|
62 |
19 (31) |
|
pT3 |
63 |
11 (17) |
|
NA |
NA |
|
63 |
11 (17) |
|
pT4 |
149 |
40 (27) |
|
NA |
NA |
|
149 |
40 (27) |
|
Mucosal or submucosal invasion depth (EGC only)b
|
LP |
NA |
NA |
NA |
62 |
29 (47) |
0.540 |
NA |
NA |
NA |
MM |
NA |
NA |
|
23 |
6 (26) |
|
NA |
NA |
|
SM1 |
NA |
NA |
|
31 |
14 (45) |
|
NA |
NA |
|
SM2 |
NA |
NA |
|
29 |
20 (69) |
|
NA |
NA |
|
SM3 |
NA |
NA |
|
39 |
16 (41) |
|
NA |
NA |
|
pN categorya
|
pN0 |
242 |
98 (40) |
< 0.001 |
165 |
77 (47) |
0.534 |
77 |
21 (27) |
0.270 |
pN1 |
52 |
20 (38) |
|
10 |
5 (50) |
|
42 |
15 (36) |
|
pN2 |
51 |
11 (22) |
|
6 |
2 (33) |
|
45 |
9 (20) |
|
pN3 |
113 |
26 (23) |
|
3 |
1 (33) |
|
110 |
25 (23) |
|
TNM stagea
|
I |
208 |
91 (44) |
< 0.001 |
175 |
82 (47) |
0.429 |
33 |
9 (27) |
0.343 |
II |
89 |
28 (31) |
|
9 |
3 (33) |
|
80 |
25 (31) |
|
III |
147 |
32 (22) |
|
0 |
0 (0) |
|
147 |
32 (22) |
|
IV |
14 |
4 (29) |
|
0 |
0 (0) |
|
14 |
4 (29) |
|
Lymphatic invasion |
Present |
150 |
45 (30) |
0.225 |
23 |
11 (48) |
0.867 |
127 |
34 (27) |
0.666 |
Absent |
308 |
110 (36) |
|
161 |
74 (46) |
|
147 |
36 (24) |
|
p53 |
Positive |
163 |
59 (36) |
0.429 |
59 |
30 (51) |
0.385 |
104 |
29 (28) |
0.488 |
Negative |
295 |
96 (33) |
|
125 |
55 (44) |
|
170 |
64 (24) |
|
Ki-67 |
Positive |
165 |
63 (38) |
0.141 |
100 |
37 (37) |
0.006 |
65 |
26 (40) |
0.002 |
Negative |
293 |
92 (31) |
|
84 |
48 (58) |
|
209 |
44 (21) |
|
Table 2.Multivariate analysis of early gastric cancer
Variable |
Parameter |
p-value |
Hazard ratio |
95% CI |
Lymph node metastasis |
Present vs absent |
0.003 |
10.162 |
2.185-47.265 |
Invasion depth |
Submucosa vs mucosa |
0.015 |
6.051 |
1.413-25.918 |
Histologic grade |
PD vs WD and MD |
0.179 |
0.313 |
0.058-1.702 |
HSP70 expresssion |
Present vs absent |
0.024 |
11.497 |
1.388-95.233 |
Ki-67 expression |
Present vs absent |
0.039 |
0.178 |
0.034-0.917 |