Journal of Pathology and Translational Medicine

Phenotypic and Genotypic Frequencies in Rh negative Koreans -An analysis of 200 cases-: Young Chul Oh, Che Kyoung Moon, Dong Wha Lee, Duk Yong Kang; J Pathol Transl Med. 1982;16(1):1-3.

1,748 View
10 Download

Abstract: The author investigated frequencies of phenotype and genotype in 200 Rh negative Koreans during 7 years from January, 1975 to September, 1981, with brief review of Japanese and Europian literatures. In 200 Rh negative Koreans, the distribution of Rh phenotypes and most likely genotypes are as follows: ccdee (cde/cde) 60%, Ccdee (Cde/cde) 23.5%, ccdEe (cdE/cde) 10%, CcdEe (Cde/cdE or CdE/cde) 40%, CCdee (Cde/Cde) 2.0% and ccdEE (cdE/cdE) 0.5%

A Study on the Serum Lysozyme Value in Patients with Leukemia: Hyon-Sok Kim, Kyung Soon Song, Samuel Y. Lee, Yun Woong Ko*; J Pathol Transl Med. 1982;16(1):4-12.

2,630 View
10 Download

Abstract: Since the Fleming's observation of bacteriolytic element in nasal mucus in 1922, lysozyme has been found to be present in various biologic fluids such as serum, urine, tear, colostrum etc. It is known to be a hydrolytic enzyme acting specifically on the structural unit of cell wall of various bacteria. In the formed elements of blood, lysozyme activity has been identified in monocytes, monoblasts and mature neutrophils and their precursors as far back as progranulocytes. Increased concentrations of lysozyme were reported in the serum and urine of patients suffering from monocytic and myelomoncytic leukemias. The activities of lysozyme are normal or decreased in lymphocytic leukemia. Attempts have been made to correlate the serum lysozyme value with diagnostic and prognostic indices of acute leukemia. However, there has been no report on the serum lysozyme study in Korea. The author measured serum lysozyme level by means of turbidimetric assay on 48 specimens from 42 patients with leukemia and 30 from normal subjects. 1) In normal subjects, the mean value of lysozyme was 7.46㎍/㎖ and the range was 3.25～11.25㎍/㎖. 2) The highest mean serum lysozyme level was found in AMMoL patients(22.7㎍/㎖). Increased level was also observed in AMoL group(16.31㎍/㎖). 3) There was some correlation between serum lysozyme level and leukocyte count but it was not significant statistically (r=0.24). 4) The mean of the ratio of serum lysozyme/WBC count per ㎕×10-3 was above 4 in AMoL. The mean of the ratio in other types of leukemia was significantly different (p<0.005). 5) When lymphadenopathy accompanied by gum hypertrophy was present in AMMoL and AMoL, an increased serum lysozyme value was also observed. 6) When initial serum lysozyme level was high, the remission rate was low in AMMoL and AMoL. From the above results, it is concluded that the measurement of serum lysozyme value is an additional guide for the diagnosis and prognosis of acute leukemia.

Effects of Storage on Immunoglobulin Concentration in Stored ACD Blood: In Sun Jun, Man Ha Huh; J Pathol Transl Med. 1982;16(1):13-18.

1,849 View
10 Download

Abstract: The present study was undertaken to assess the effects of storage on the stability of plasma immunoglobulins(IgG, IgA, IgM and IgD) in ACD stored blood after periods of storage up to 12 weeks at 4℃. 1) Blood was drawn from 5 healthy volunteer donors into acid-citrate-dextrose(ACD) anticoagulant containers and placed in the blood bank refrigerator. Plasma immunoglobulins were measured on all samples initially and every week up to 12 weeks of storage at 4℃. Plasma immunoglobulins were measured by single radial immunodiffusion method. 2) Results of analyses are presented and discussed. The coefficient of variation of test samples is compared with that of standard quality control data (IgG: 8.8%, IgA: 7.9%, IgM: 9.5% and IgD: 9.3%) 3) No significant differences were found in the concentrations of immunoglobulins compared with the initial concentrations at the 3rd week (expiration date) and the 12th week.

Clinicopathological Study of Primary Mucinous Ovarian Tumor According to the Number of Mitosis: Chan Il Park, Dong Whan Shin, In Joon Choi, Myung Sook Yoo*, In Seo Park*, Hyo Sook Park**; J Pathol Transl Med. 1982;16(1):19-26.

2,176 View
11 Download

Abstract: After Scully divided the primary ovarian epithelial tumors into benign, borderline and malignant varieties, it has been repeatedly emphasized that there are appreciable differences in terms of prognosis in each group. However there may be individual difference among examiners when the pathological diagnosis is made on the basis of complex interpretation of the nuclear pleomorphism, mode of proliferation, frequency of mitosis and the presence of stromal invasion. In this study histological grading was made according to the frequency of mitosis, the most reliable index for the rate of cellular proliferation, in 98 mucinous ovarian tumors. Of the 98 subjects 80 cases belonged to the grade I (0～1 mitosis/HPF), and the grade II(2～3/HPF), III (4～5/HPF) and IV (> 5/HPF) tumors were seen in 11, 5 and 2 cases respectively. The degree of nuclear pleomorphism and the mode of cellular proliferation, such as the frequency and degree of papillary growth and secondary gland formation, corresponded well to the grades of tumors. Among the 98 mucinous tumors, seven cases (7.1%) showing stromal invasion were classified as malignant, irrespective of histologic grades, and all the grade I tumors but those with stromal invasion was grouped into benign, which comprised 80.6%. The remaining tumors were categorized into borderline malinancy which comprised 12.3% of all. The grade IV tumors showed smaller size and less multilocularity than the lower grade ones, suggesting that the higher-grade tumors tend to grow as solid mass rather than cystic locules. The short symptom-duration as well as the bilateral involvement were more likely of higher grade tumors. It was emphasized that one must pay regard to the frequency of mitosis when primary mucinous ovarian tumors were dealt with.

Ultrastructural changes of alveolar capilary endothelial cells induced by acute oxygen poisoning: Jong Min Chae, Jyung Sik Kwak, Tae Joong Sohn; J Pathol Transl Med. 1982;16(1):27-32.

1,671 View
10 Download

Abstract: The author carried out light microscopic and electron microscopic studies of alveolar capillary endothelial cells of rabbits after inhalation of pure oxygen in order to investigate oxygen toxicity on the endothelial cells and periodically observed after inhalation for 24,48,72,96, and 120 hours. The results were as follows. Light microscopically, congestion, edema, focal hemorrhage, and focal inflammatory infiltrates occurred within 24 hours of oxygen inhalation. Capillary proliferation and thickening of alveolar septa occurred at 72 hours of inhalation. Over 96 hours of inhalation thrombosis and focal necrosis appeared. Electron microscopically, pseudopod formation at 24 hours; mild swelling of Golgi apparatus and vacuolization of cytoplasm at 72 hours; loss of pseudopods, destruction and bleb formation of cell membrane, mitochondrial swelling, vacuolization of cytoplasm, and necrosis over 96 hours after oxygen inhalation appeared. Severity of degradation was more pronounced in the plasma membrane than in the cell organelles. From the above results, it is conceived that the endothelial cells fall in irritable state within 24 hours of pure oxygen inhalation and have irreversible injury over 96 hours of inhalation and that primary site of injury induced by oxygen toxicity is plasma membrane.

Reclassification of Malignant Lymphomas in Korean Patients According to Lukes and Collins Classification: Woo Hee Jung, Chan Il Park, Yoo Bock Lee; J Pathol Transl Med. 1982;16(1):33-42.

2,221 View
10 Download

Abstract: Since Thomas Hodgkin described tumors of the lymph nodes in 1832, morphologic classification of malignant lymphoma has been studied by various approached, and among them, the classification of Rappaport has been most widely accepted. But his approach entirely depended on morphology which failed to properly reflect the functional aspect of the component cells. With the recent development in the immunologic knwoledge of the lymphoreticular system and more acurate definition of various cells by histochemical methods, Lukes and Collins initiated a new approach to the classification of the malignant lymphomas taking the functional and histochemical aspects into consideration in 1974. For its proven usefulness in the clinical prognostication of the malignant lymphomas, this new concept has been widely disseminated. However, in Korea the old classification of Rappaport is still being used for largely technical reasons. In view of the recent trends of development in this important field, the author has made attempts at reclassification of the malignant lymphomas in Korea. The material consists of 142 cases of malignant lymphomas examined at the Department of Pathology, Yonsei University College of Medicine from January, 1975 through August, 1980. All cases were subjected to histopathological analysis and review of the clinical records. The paraffin blocks were sectioned serially at 5 micron thickness and sections were stained with hematoxylin and eosin and methyl green pyronin(MGP), and were examined by light microscopy up to the magnification of 1000 times. 1) Among 142 cases of malignant lymphomas, 111 cases (78%) were follicular enter cell type, and of the latter, 40 cases (28.2%) were "large noncleaved" type and 33 cases (23.2%) were "large cleaved" type. 2) Of the seventy-seven cases formerly diagnosed as "histiocytic" type according to Rappaport classification, 23 cases turned out to be "large cleaved" type and 22 cases, "large noncleaved" type. Thus 58.4% of the 77 cases proved to be of "large cell" line by the new approach. 3) There were no cases belonging to S?zary syndrome related to mycosis fungoides and small lymphocytic lymphomas in this study. 4) Of 130 cases of the malignant lymphomas in which the pattern of cell arrangement was clearly discernible, 97 cases (74.6%) demonstrated diffuse pattern of lymphomatous proliferation, and it was noted that all of the cases of plasmacytoid lymphocytic lymphomas and "small noncleaved" type, and also most of the cases of convoluted lymphocytic lymphoma, "large noncleaved" type and immunoblastic sarcoma showed this diffuse pattern. 5) Convoluted lymphocytic lymphoma predominantly occurred in adolescent and young adult male characteristically showing mediastinal mass, and the prognosis was poor because of generalized manifestation at the time of clinical diagnosis and the tendency of conversion to acute lymphoblastic leukemia. 6) While three quarters of the cases of "small cleaved", "large cleaved" and "large noncleaved" type showed partial or complete remission, most of the cases belonging to convoluted lymphocytic lymphoma and immunoblastic sarcoma resulted in poor prognosis clinically.

An Experimental Study on the Changes of Serum Lipids in Rabbits with the Posthemorrhagic Anemia: Jin Q Kim; J Pathol Transl Med. 1982;16(1):43-51.

1,712 View
10 Download

Abstract: Serum lipids were estimated in 17 healthy rabbits as acontrol group and 9 rabbits with the experimentally induced posthemorrhagic anemia. Anemia was induced in rabbits by serial phlebotomy with the three to four days interval. At each time of the phlebotomy, about ten percent of the total blood volume was drawn out from the rabbits and the phlebotomy was performed six times during the study. The anemic group consisted of low hemoglobin group and low hematocrit group compared with the control group. Both the anemic groups of the rabbits had low serum lipids levels with a mean of 57 to 87 percent of the controls. A positive correlation was established in male anemic rabbits for hemoglobin and hematocrit with the HDL-Cholesterol, cholesterol, Total lipid and phospholipid, but not with Triglycerides. Female anemic rabbits remarked the positive correlation only with the Cholesterol. The correlation of reticulocytes production with the decrease of the serum lipids was not significant in statistics. This study shows the correlation of hematocrit decrease and hypocholesterolemia was more significant than that of the hemoglobin and hypocholesterolemia. In conclusion, plasma dilutional effect was considered to be an of the most plausible explanations as the mechanism of the hypolipidemia accompanying anemia, however, there may be several other contributory factors with complexities that should be studied.

Malignant Schwannoma -16 cases analysis-: Jung Ran Kim, Je G Chi, Eui Keun Ham; J Pathol Transl Med. 1982;16(1):52-59.

1,333 View
10 Download

Abstract: A histopathological study was done on 16 cases of malignant Schwannoma that were diagnosed at the Department of Pathology, Seoul National University during a period of 13 years, from January 1968 to December 1980. The 16 malignant Schwannomas were distributed in the mediastinum(5), extremities(4), abdominal cavity(2), retroperitoneum(1), etc. The peripheral nerve of origin was identified in four cases, and these were sciatic(2), femoral(1) and musculocutaneous(1). In two cases, electron microscopy was done and the Schwann cell origin was strongly supported by the ultrastructual findings of the tumors showing basement membranes, interdigitated intercellular junction and desmosomes. Histopathological features on malignant Schwannoma were characterized by mesodermal elements that were intermingling with Schwannian elements, such as cartilage, osteoid and rhabdomyoblasts. Two cases of malignant Schwannoma revealed eosinophilic granular cells in the midst of the tumor tissue. These cells demonstrated secondary lysosome in the cytoplasm electronmicroscopically.

Primary Choriocarcinoma of the Ovary -a case report and literature review-: Giu Ran Kim, In Sun Kim, Seung Yong Paik; J Pathol Transl Med. 1982;16(1):60-63.

2,227 View
10 Download

Abstract: Ovarian choriocarcinoma may either be primary or metastatic from the uterus and oviduct. Gestational choriocarcinoma following an ectopic pregnancy of the ovary are usually pure in composition, however, the tumors which are nongestational and teratomatous in origin, frequently combine with other germ cell tumors such as dysgerminoma, immature teratoma, embryonal carcinoma, endodermal sinus tumor, or teratocarcinoma. Primary choriocarcinoma of the ovary is extremely rare and only occasional case reports are found in the literature. The authors reported a case of primary nongestational choriocarcinoma combined with immature teratoma which arose in an ovary of 17-year old female.

Extraskeletal Ewing's Sarcoma: Young Chun Moon, Jung Il Suh, Moon Hyang Park, Hyo Sook Park; J Pathol Transl Med. 1982;16(1):64-67.

1,482 View
10 Download

Abstract: A case of extraosseous soft tissue tumor with characteristics of Ewing's sarcoma of bone in 14 year old girl is reported. This patient was admitted with a painful mass on the left calf area without limitation of motion. A brief review of literature is added.

Adenocarcinoma of the Jejunum -A case report-: Sang Sook Lee, Chai Hong Chung; J Pathol Transl Med. 1982;16(1):68-71.

1,781 View
10 Download

Abstract: Malignant lesions of the small intestine are rare, averaging only 1% of GI carcinoma. The most common primary malignancy of the small intestine is adenocarcinoma, comprising approximately 40～50% of the total malignant neoplasms of the small intestine. The most frequently affected sites are the duodenum(40%), jejunum(38%) and ileum in that order. Mortality from the malignant lesions of the small intestine is higher than that from tumors involving any other portion of the gastrointestinal tract. We have recently experienced a case of adenocarcinoma of the jejunum in a 70-y-o male patient, which herein reported and the literatures are reviewed.

Congenital Dermal Sinus of the Posterior Cranial Fossa: Eun Deuk Chang, Sang In Shim, Chong Moo Lee; J Pathol Transl Med. 1982;16(1):72-75.

1,660 View
10 Download

Abstract: Congenital dermal sinus is sinus is any depression or tract lined with stratified squamous epithelium which apparently result from defective separation of the neuroectoderm from the epithelial ectoderm along the mid-dorsal aspect of the embryo between the third and fifth week of intrauterine life. It occurs most frequently in the lumbosacral region and the next in the occiput. This dermal sinus may extends all the way into the spinal canal or cranial cavity. Because of its persistent communication with the skin surface, such a sinus tract serves as a constant potential port of entry for infection into the central nervous system. We have experienced a case of occipital dermal sinus of 5 months old male baby, who was admitted to Catholic Medical CЦ픔?Ч퉘?or high fever, vomiting and a small palpale mass at the occipital scalp. Computed tomography showed a round low density lesion with ring enhancement in the posterior fossa. An operation was given to reveal sinus tract started at the skin and extended into the posterior fossa forming a cyst, 4㎝ in diameter, which was located between cerebellar hemispheres. The cyst was filled with a yellowish white cheesy material containing hairs. Microscopically, the cyst was lined by stratified squamous epithelium and appendageal structures such as hair follicles and sweat glands. The sinus tract was also lined with stratified squamous epithelium. Total excision of the cyst and dermal sinus tract was performed, and the postoperative course was uneventful.

A Case of Malignant Lymphoma Terminating in Acute Nonlymphocytic Leukemia -A case report-: Ho Jong Chun, Myung Hee Lee, Young Jin Park; J Pathol Transl Med. 1982;16(1):76-85.

1,433 View
10 Download

Abstract: A patient with diffuse, well-differentiated malignant lymphoma later developed acute nonlymphocytic leukemia(ANLL) 9 months following the diagnosis. The patient received radiotherapy and long term courses of alkylating agent therapy for the malignant lymphoma. Malignant lymphoma at the diagnosis of leukemia relapsed and developed in generalized lymph nodes, liver and spleen. The ANLL was signalled by increasing anemia and thrombocytopenia. With an intensive chemotherapy for the ANLL, however, the survival from the leukemia was one month. A case of ANLL developing after radiotherapy and chemotherapy for a well differentiated malignant lymphoma were discussed with brief current literature review.

Extraskeletal Ewing's Sarcoma: Jung Ran Kim, Geung Hwan Ahn; J Pathol Transl Med. 1982;16(1):86-89.

1,519 View
10 Download

Abstract: Thirty nine cases of rhabdomyosarcoma and unclassified sarcoma as of January, 1971 to December, 1980 were reviewed. On critical analysis three cases of soft tissue tumor resembling Ewing's sarcoma of bone were noted. Primary sites were right orbit, right popliteal fossa and right buttock. The patients ranged from 5 months to 36 years of age. These tumors consisted of monotonous tumor cells with round-to-oval nuclei and scant pale-staining indistinct cytoplasm. The nuclei had fine dispersed powdery chromatin with indistinct nucleoli. Glycogen was demonstrated in all case. According to this analysis extraskeletal Ewing's sarcoma seemed to be a distinct clinicopathological entity. Differential diagnoses and histogenesis of extraskeletal Ewing's sarcoma were presented.

Two Cases Cis-A2B Blood Group Family -unusual inheritance of ABO blood groups-: Young Chul Oh, Won Bae Kim, Che Kyong Moon, Dong Wha Lee,g Duk Yong Kang; J Pathol Transl Med. 1982;16(1):90-94.

1,679 View
10 Download

Abstract: Generally, a group AB person cannot be the parent of a group O child, nor can a oup O person be the parent of a group AB child. But, rarely cases of an A2B child born to parents of group A2B and O or of group A2B and A1 were observed, and the most entertaining explanation for those cases were inheritance of A and B on one chromosome. For this unusal inheritance, the notation "cis-AB" was proposed. Recently, thors experienced two family cases of cis-A2B and were reported with a brief review of literatures.

A Case of Microglioma: An Hi Lee, Seok Jin Gang, Chong Moo Lee; J Pathol Transl Med. 1982;16(1):95-97.

1,781 View
10 Download

Abstract: A case of microglioma of the brain in a 48-year-old man, diagnosed by the histopathological examination of the tumor mass removed by surgical operation was presented. The tumor with somewhat ill defined outlines was located at the left frontal inferior portion of the right cerebellum. Microscopically, there was diffuse infiltration of the brain with tumor cells forming collars around the blood vessels. Various tumor cell elements are mostly mixed with lymphocytes. Gomori's silver stain reveal an increased perivascular reticulin fibers and splitting of the reticulin fibers by the tumor cells.

Granular Cell Tumor of Bronchus: Eun Yup Lee, Soo Kun Kim, Han Chul Son, Soon Ho Kim; J Pathol Transl Med. 1982;16(1):98-102.

1,955 View
10 Download

Abstract: Granular cell tumor is relatively uncommon lesion appearantly peculiar to man with uncertain histogenesis. It arises most commonly in the tongue but the bronchus is rarely the site of this tumor. The present paper is a report on a case of granular cell tumor arising from the left lower lobe bronchus. 29-year old Korean women was admitted to BNUH on November, 1978, because of productive cough, hemophtysis and chest pain for 15 years. She was well until 15 year of age, when she had been treated with drugs, under the diagnosis of pulmonary tuberculosis for 2 years with no effect at local clinic. The physical examination on admission revealed moist coarse rales on the left lower lung field. An X-ray films of the chest showed radioopaque density with peumonic infiltration on the left lower lobe. Bronchogram revealed bronchiectasis at this lesion. Under the diagnosis of bronchiectasis, the left pneumonectomy was performed. Grossly the left lung was not remarkable except for a palbable small firm mass on the left lower lobe. On section, there was a small peduncleated polypoid gray white mass within the left bronchus with partial obstruction of its lumen. The neoplasm extended through the wall of the bronchus to peribronchial tissue and adjacent lung parenchyma. It measured about 2㎝ in the long diameter. Histologically the neoplasm was consisted of diffuse sheets of large oval to polygonal cells that had abundant fine granular cytoplasm and a small central to eccentric nuclei. The cells were disposed in small groups, separately form each other by delicated fibrous stroma. The full thichness of the underlying wall of the bronchus, peribronchieal tissue and even adjacent pulmonary tissue were diffusely infiltrated by tumor cells.

H?rthle Cell Adenoma of the Thyroid -Ultrastructural study-: Jong Min Chae, Jung Sik Kwak, Tae Jung Sohn; J Pathol Transl Med. 1982;16(1):103-106.

1,568 View
10 Download

Abstract: H?rthle cell adenoma of the thyroid gland is a morphologically peculiar tumor. The tumor cells have numberous mitochondria and scanty cell organelles and show strong eosinophilicity on hematoxylin and eosin stain. A case of H?rthle cell adenoma of the thyroid gland was studied by light and electron microscopy. The most distinctive ultrastructural feature was also mitochondrial abundancy with occasional admixture of cystically dilated rough endoplasmic reticulums. The authors discussed the ultrastructure of H?rthle cell adenoma with a review of literatures.

Gastric Hyperplastic Adenomatous Polyposis: Myoung Hee Park, Woon Sup Han, Ok Kyoung Kim, Seung Haeng Joo*, Kyun Il Yoon*; J Pathol Transl Med. 1982;16(1):107-110.

1,254 View
10 Download

Abstract: Gastric polyps could be subdivided microscopically into hyperplastic adenomatous and papillary adenoma type. Hyperplastic adenomatous polyp is relatively more common and rarely associated with carcinoma. A case of multiple gastric polyps which associated with severe atypism in 45-years-old man is presented. The case was the hyperplastic adenomatous polyp. A brief review of the literature was made.

Chylangioma of the Mesentery -A Case Report-: Yong Wook Park*, Jae Hyung Yoo, Kye Yong Song; J Pathol Transl Med. 1982;16(1):111-116.

1,934 View
10 Download

Abstract: Lymphangioma (chylangioma) of the mesentery is one of the extremely rare tumor and only a few cases were reported in the literature as chylous cyst, mesenteric cyst and cystic lymphangioma. Recently, authors experienced a huge cavernous lymphangioma of the mesentery of the jejunum and herein reported with literature review. The patient was a 27 year old Korean female who admitted to the Hospital on Aug. 4, 1981 because of colicky abdominal pain at postpartum 3rd day. She had been experienced several epidoses of intermittent colicky abdominal pain since Feb. '81. Routine laboratory findings were within normal limits except simple abdomen X-ray revealed step ladder pattern suggesting mechanical intestinal obstruction. At operation a huge partly cystic and grayish white soft mass along the mesenteric border was noted. So segmental resection of jejunum with its mesenteric mass was performed and end to end anastomosis was done. Hospital course was good and uneventful. Submitted specimen consisted of a 55㎝. long small bowel (jejunum) with attached mesenteric mass, measuring 25×10.5×6㎝. in maximum dimension and its broad attachment to the mesenteric border of jejunum was 43㎝. Cut surface was smooth and grayish white with doughy consistency. Cut surface exhibited both multicystic spaces with milkish chyle and yellowish honeyomb portions. Multiple polypoid elevations and hypertrophy of mucosa of jejunum were also noted. Microscopic findings were those of typical cavernous lymphangioma involving both jejunum and its mesentery.

Previous issues