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Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips
So-Woon Kim, Jin Roh, Chan-Sik Park
J Pathol Transl Med. 2016;50(6):411-418.   Published online October 13, 2016
DOI: https://doi.org/10.4132/jptm.2016.08.08
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AbstractAbstract PDF
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication.

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Original Article
CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin
Gawon Choi, Jin Roh, Chan-Sik Park
J Pathol Transl Med. 2016;50(5):361-368.   Published online August 7, 2016
DOI: https://doi.org/10.4132/jptm.2016.06.19
  • 8,388 View
  • 118 Download
  • 5 Web of Science
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AbstractAbstract PDF
Background
CD99 is a cell surface transmembrane glycoprotein expressed in various tissues. CD99 is differentially expressed between subpopulations of each tissue and is highly expressed in certain hematopoietic and precursor cells. However, there has been no comprehensive study of CD99 expression in normal skin. We evaluated CD99 expression in normal human skin and developing fetal skin.
Methods
Seventy-five adult skin samples containing normal skin and eight fetal skin samples of different gestational ages were collected. CD99 immunohistochemical staining was performed to evaluate expression pattern in adult and fetal skin samples. CD99 and CD34 expression were compared by double immunofluorescence.
Results
In normal adult skin, CD99 was strongly expressed in the membrane of epidermal basal keratinocytes, hair follicle bulges and outer root sheaths, and inner secretory cells of eccrine sweat glands. In fetal skin, CD99 was not expressed on the periderm at 16 weeks of gestation but was expressed in basal cells of fetal skin at around 19 weeks of gestation. CD99 expression became comparable to that of the adult skin after 20 weeks of gestation. CD99 and CD34 were co-expressed in hair follicle outer root sheaths, as seen by double immunofluorescence study.
Conclusions
This is the first study examining CD99 expression pattern in normal adult and fetal skin. CD99 tends to be expressed in the basal/precursor cells of epidermis and in hair follicles. These results provide a basis for future investigation on functions of CD99 in the skin and provide a novel potential target for the treatment of dermatologic lesions.

Citations

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  • Childhood pilomatrixoma mimicking malignant small round blue cell tumor with positivity for CD99: Potential pitfall in cytology
    Brijdeep Singh, Radhika Srinivasan, Deepak Bansal, Manish Rohilla, Pranab Dey, Uma Nahar Saikia, Ritambhra Nada
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  • Role of CD99 in regulating homeostasis and differentiation in normal human epidermal keratinocytes
    Yi Li Wong, Toru Okubo, Eiko Uno, Kazuma Suda, Tsuyoshi Ishii
    Biochemical and Biophysical Research Communications.2022; 606: 108.     CrossRef
  • Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma
    Nuchjira Takheaw, Gunya Sittithumcharee, Ryusho Kariya, Watchara Kasinrerk, Seiji Okada
    Cancer Immunology, Immunotherapy.2021; 70(6): 1557.     CrossRef
  • “Neuroectodermal influence of CD 99 immunoexpression correlates with the clinical behavior of odontogenic cysts and tumors”
    Harshi Mishra, Nikita Gulati, Anshi Jain, Saurabh Juneja, Devi Charan Shetty
    Journal of Oral and Maxillofacial Pathology.2021; 25(3): 423.     CrossRef
  • CD99 at the crossroads of physiology and pathology
    Michela Pasello, Maria Cristina Manara, Katia Scotlandi
    Journal of Cell Communication and Signaling.2018; 12(1): 55.     CrossRef
  • CD99: A Cell Surface Protein with an Oncojanus Role in Tumors
    Maria Manara, Michela Pasello, Katia Scotlandi
    Genes.2018; 9(3): 159.     CrossRef
Brief Case Reports
Clear Cell Papulosis: A Case Report
So-Woon Kim, Jin Roh, Chan-Sik Park
J Pathol Transl Med. 2016;50(5):401-403.   Published online May 29, 2016
DOI: https://doi.org/10.4132/jptm.2016.02.16
  • 8,555 View
  • 126 Download
  • 4 Web of Science
  • 6 Crossref
PDF

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J Pathol Transl Med. 2016;50(6):479-481.   Published online May 9, 2016
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J Pathol Transl Med. 2016;50(1):78-81.   Published online August 4, 2015
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Solid Form of Epithelioid Hemangioma: A Case Report
Jin Roh, Min Jeong Song, Mi Woo Lee, Chan-Sik Park
Korean J Pathol. 2014;48(5):394-397.   Published online October 27, 2014
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Review
Current Concepts in Primary Effusion Lymphoma and Other Effusion-Based Lymphomas
Yoonjung Kim, Chan Jeong Park, Jin Roh, Jooryung Huh
Korean J Pathol. 2014;48(2):81-90.   Published online April 28, 2014
DOI: https://doi.org/10.4132/KoreanJPathol.2014.48.2.81
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AbstractAbstract PDF

Primary effusion lymphoma (PEL) is a human herpes virus 8 (HHV8)-positive large B-cell neoplasm that presents as an effusion with no detectable tumor in individuals with human immunodeficiency virus infection or other immune deficiencies. PEL is an aggressive neoplasm with a poor prognosis. PEL cells show diverse morphologies, ranging from immunoblastic or plasmablastic to anaplastic. The immunophenotype of PEL is distinct, but its lineage can be misdiagnosed if not assessed thoroughly. PEL cells usually express CD45, lack B- and T-cell-associated antigens, and characteristically express lymphocyte activation antigens and plasma cell-associated antigens. Diagnosis of PEL often requires the demonstration of a B-cell genotype. HHV8 must be detected in cells to diagnose PEL. In most cases, PEL cells also harbor the Epstein-Barr virus (EBV) genome. Similar conditions associated with HHV8 but not effusion-based are called "extracavitary PELs." PELs should be differentiated from HHV8-negative, EBV-positive, body cavity-based lymphomas in patients with long-standing chronic inflammation; the latter can occur in tuberculous pleuritis, artificial pneumothorax, chronic liver disease and various other conditions. Despite their morphological similarity, these various lymphomas require different therapeutic strategies and have different prognostic implications. Correct diagnosis is essential to manage and predict the outcome of patients with PEL and related disorders.

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Original Article
Prognostic Significance of Absolute Lymphocyte Count/Absolute Monocyte Count Ratio at Diagnosis in Patients with Multiple Myeloma
Su-Jin Shin, Jin Roh, Misung Kim, Min Jung Jung, Young Wha Koh, Chan-Sik Park, Dok Hyun Yoon, Cheolwon Suh, Chan-Jeong Park, Hyun Sook Chi, Jooryung Huh
Korean J Pathol. 2013;47(6):526-533.   Published online December 24, 2013
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AbstractAbstract PDF
Background

Absolute lymphocyte count (ALC) in peripheral blood has recently been reported to be an independent prognostic factor in multiple myeloma (MM). Previous studies indicated that the absolute monocyte count (AMC) in peripheral blood reflects the state of the tumor microenvironment in lymphomas. Neither the utility of the AMC nor its relationship with ALC has been studied in MM.

Methods

The prognostic value of ALC, AMC, and the ALC/AMC ratio at the time of diagnosis was retrospectively examined in 189 patients with MM.

Results

On univariate analysis, low ALC (<1,400 cells/µL), high AMC (≥490 cells/µL), and low ALC/AMC ratio (<2.9) were correlated with worse overall survival (OS) (p=.002, p=.038, and p=.001, respectively). On multivariate analysis, the ALC/AMC ratio was an independent prognostic factor (p=.047), whereas ALC and AMC were no longer statistical significant. Low ALC, high AMC, and low ALC/AMC ratio were associated with poor prognostic factors such as high International Staging System stage, plasmablastic morphology, hypoalbuminemia, and high β2-microglobulin.

Conclusions

Univariate analysis demonstrated that changes in ALC, AMC, and the ALC/AMC ratio are associated with patient survival in MM. Multivariate analysis showed that, of these factors, the ALC/AMC ratio was an independent prognostic factor for OS.

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