Fig. 1(A-C) Boxplots of raw data in D5S346, D17S250, and D2S123, respectively. (D-F) Scatter plot and regression line in each marker. (G-I) Boxplots of the calibrated data in each marker (The X-axis and Y-axis represent the estimated nucleotide length (ENL) and difference between observed nucleotide length and ENL, respectively).
Fig. 2Results of the GeneScan analysis in case 1, 2, and 3. (A) Case 1. (B) Case 2. (C) Case 3.
Fig. 3Example of cases of a specimen mix-up. (A) Among the several fragmented specimens, one fragment (arrow) is composed of benign spindle cells and the remaining portions are carcinoma. (B) A high magnification view of the tissue in Fig. 3A (arrow portion). (C) A high magnification view of the carcinoma portion. (D) The result of microsatellite instability test in two different tissues. Upper, GeneScan of benign spindle cell lesion; lower, GeneScan of carcinoma portion. There is a difference between the allele patterns.
Table 1PCR primers for the microsatellite instability test
Table 2Regression coefficients and statistical parameters in three loci: D5S346, D17S250, and D2S123
Table 3Size of alleles and allele frequencies in D5S349, D17S250, and D2S123
Table 4Statistical parameters in D5S349, D17S250, and D2S123
Table 5Examples of random match probability and likelihood ratio in each allele